A Phenotypic Profile of the Candida albicans Regulatory Network
(A) 166 transcriptional regulators were knocked out using homologous recombination. Signature-tagged markers (stars) were included for use in future analyses. (B) The phenotyping screen consisted of a large primary screen and several smaller supplemental screens. (C) Photographs of phenotyping media were processed, archived, and scored using custom Java software. Phenotyping scores were then compiled for each combination of knockout and phenotyping medium and exported to a spreadsheet for further analysis.