The Synaptonemal Complex Protein Zip1 Promotes Bi-Orientation of Centromeres at Meiosis I
Figure 2
Zip1p localizes to both paired and unpaired non-exchange chromosomes.
A strain (DD728) carrying a GFP-tagged non-exchange chromosome V pair was induced to enter meiosis at 23°C. Thirteen hours following meiotic induction, chromosome spreads were prepared. Zip1p morphology and the pairing of GFP-tagged centromeres of non-exchange chromosomes were evaluated using indirect immunofluorescence microscopy. Shown are representative chromosome spreads with continuous Zip1 staining. Both paired and unpaired non-exchange chromosomes were typically decorated with Zip1p. Size bar equals 5 µm.