The Euchromatic and Heterochromatic Landscapes Are Shaped by Antagonizing Effects of Transcription on H2A.Z Deposition
Figure 1
Human H2A.Z associates with promoters early during the transcription process.
(A) A snapshot showing H2A.Z enrichment (red) along a 1 Mb region from chromosome 19. Chromosomal coordinates (in kb) are shown on top and genes are shown at the bottom. Double-headed dashed arrows are pointing to H2A.Z peaks that map to gene promoters (blue) and other elements (green). (B) Mapping of RNAPII enrichment on groups of genes defined by their occupancy of RNAPII at their promoter (Figure S3A). (C) Mapping of H2A.Z on the same groups of genes as panel B. (D) Mapping of P-Ser2 RNAPII enrichment on groups of genes defined as follow: genes with high RNAPII occupancy at their promoter (the union of the red, orange and yellow genes from panel B were ranked based on their P-Ser2 RNAPII level and then separated into four groups. The red genes correspond to genes with high level of RNAPII and high level of P-Ser2 RNAPII. The blue genes have high level of RNAPII but low level of P-Ser2 RNAPII. (E) H2A.Z mapping on the gene groups defined in panel D. (F) RNAPII and H2A.Z enrichment over time after dauno treatment in serum-deprived (G1/G0-arrested) cells on the promoter of IL8 (left) and CCL2 (right). Note that a version of that figure where all ZNF genes were removed is shown as Figure S12).