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Parallel Germline Infiltration of a Lentivirus in Two Malagasy Lemurs

Figure 5

PCR screening for presence/absence of orthologous solo LTR in various species of Malagasy lemurs.

For each locus, the larger PCR product indicates presence of the LTR, the smaller product indicates absence. (A) Primers (6160F: 5′-CAG CAK TTT CAT CAG CAA TTT G; 6160R: 5′-GCA AGC TGT GMC ACA TTT ATT BGC) were designed on the regions flanking the solo LTR in contig ABDC01306160. The expected size was ∼670 bp for presence and ∼250 bp for absence. (B) Primers (9233F: 5′-ATC TRT AGT CAA ATC CTG GG; 9233R: 5′-TAA TAC TCA CAA AAA CYT TAC C) were designed on the regions flanking the solo LTR in contig ABDC01159233. The expected size was ∼550 bp for presence and ∼130 bp for absence. (C) Primers (61523F: 5′-AAA TGA GTT TTG TTG CTC TRT YTC; 61523R: 5′-ATG TTR CTT TGG GTA GMT TG) were designed on the regions flanking the solo LTR in contig ABDC01361523. The expected size was ∼585 bp for presence and ∼165 bp for absence. The genus Eulemur and Propithecus belong to the family Lemuridae and Indriidae respectively. All the other species (genera Cheirogaleus, Microcebus and Mirza) belong to the family Cheirogaleidae. The tree depicts the phylogenetic relationships of the species and their divergence times according to [23],[41]. See Table S4 for the voucher specimen numbers of the lemur samples used in this study.

Figure 5

doi: https://doi.org/10.1371/journal.pgen.1000425.g005