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The Anticonvulsant Ethosuximide Disrupts Sensory Function to Extend C. elegans Lifespan

Figure 5

osm-3(p802) animals are sensitive to egg-laying stimulated by serotonin and ethosuximide.

(A) Gravid hermaphrodites were placed individually in M9 buffer containing the indicated serotonin concentration, and the number of eggs laid in 90 minutes was scored. The mean number of eggs laid±SD by wild-type animals (blue circles) was 0.6±1.2, 1.4±1.8, 7.3±4.9, and 5.7±4.3 for serotonin concentrations of 0, 1, 10, and 20 mM, respectively. The mean number of eggs laid±SD by osm-3(p802) animals (red squares) was 0.6±1.7, 0.3±0.9, 4.8±3.5, and 7.1±3.4 for serotonin concentrations of 0, 1, 10, and 20 mM, respectively. N>11 for all trials. At all serotonin concentrations, differences in egg-laying between WT and osm-3 were not statistically significant, p>0.05 (Student t-test). (B) Experiments with ethosuximide were carried out as above with the indicated concentration of ethosuximide. The mean number of eggs laid±SD by wild-type animals (blue circles) was 0.2±0.5, 1.1±1.4, 4.5±4.2, and 7.6±3.6 for ethosuximide concentrations of 0, 4, 8, and 12 mg/ml, respectively. The mean number of eggs laid±SD by osm-3 mutant animals (red squares) was 0.3±0.8, 1.7±2.6, 6.0±4.3, and 9.6±2.8 for ethosuximide concentrations of 0, 4, 8, and 12 mg/ml, respectively. n = 24 for all trials. Differences in egg-laying between WT and osm-3 at ethosuximide concentrations of 0, 4, and 8 were not statistically significant, p>0.05 (Student T-test). At 12 mg/ml ethosuximide, osm-3 animals laid significantly more eggs than wild-type animals, p = 0.03 (Student T-test).

Figure 5

doi: https://doi.org/10.1371/journal.pgen.1000230.g005