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The Anticonvulsant Ethosuximide Disrupts Sensory Function to Extend C. elegans Lifespan

Figure 3

Ethosuximide treated animals and che-3(am178) mutants cultured at 20°C did not display dye-filling defects.

Animals were incubated in the lipophilic dye DiO and observed using fluorescence microscopy at 200× magnification. Each panel illustrates the anterior tip of the animal (top) to the base of the bi-lobed pharynx (bottom). DiO stains amphid neuron processes (arrows) and cell bodies (arrowheads). Wild-type (WT) animals displayed robust staining that was not affected by treatment with 4 mg/ml ethosuximide (WT+ETH4). che-3(am178) mutants cultured at 20°C displayed robust staining, whereas osm-3(p802) mutants displayed no detectable staining (the Dyf phenotype).

Figure 3