Efficient Genetic Method for Establishing Drosophila Cell Lines Unlocks the Potential to Create Lines of Specific Genotypes
Figure 6
Use of RasV12 expression to generate cell lines expressing a wtsRNAi transgene.
The RasV12 wtsRNAi cells are larger than RasV12 cells and primarily tetraploid. (A) RasV12 cells from line 11, which are predominantly diploid (94%). (B) Cells from RasV12 wtsRNAi line 10, which are predominantly tetraploid (84%) and relatively large (compare cell size in A and B). (C) Histogram showing ploidy of various cell lines (green, % diploid; blue, % triploid; red, % tetraploid). RasV12 wtsRNAi cells are significantly more polyploid than wild type (p = 0.001) and RasV12 cells (p = 0.007). (D–F) Chromosome spreads of diploid, triploid, and tetraploid cells, respectively. The small 4th chromosome is often lost in cells in culture and/or not visible in karyotype spreads. (G) RasV12 -line 10 expresses dMef suggesting it is of mesodermal origin. (H) Confocal image of RasV12; wtsRNAi cells. The cells have an epithelial-like morphology and expresses E-Cadherin. (Scale bar (B), 50 µm in A and B).