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High-Precision, Whole-Genome Sequencing of Laboratory Strains Facilitates Genetic Studies

Figure 2

Quality analysis of sequences generated with the Illumina/Solexa platform.

Sequencing errors were estimated by self-against-self comparison tests. Independent Solexa reads from a single 168 sample were randomly shuffled and split into two equal groups. Consensus sequences from these two groups were compared, and the experiment was repeated 10 times to calculate the average number of errors. The observed average number of errors decreased exponentially with the consensus quality score calculated by MAQ.

Figure 2