High-Precision, Whole-Genome Sequencing of Laboratory Strains Facilitates Genetic Studies
A) B. subtilis 168 (BGSC 1A700); B) NCIB 3610 (BGSC 3A1); C) SMY (BGSC 1A775); D) JH642 (BGSC 1A96); E) JH642 with relA deletion; F) JH642 with relA deletion and second site suppressors (relA-*). Yellow arrows: 18 kb deletion (ydzA- ydbA) in JH642; purple arrows: 9 kb deletion (ppsC- ppsD) in JH642; green arrows: 2 kb relA deletion in strains relA- and relA-*; blue arrow: skin element deletion in relA-*. Coverage was obtained by calculating the average number of reads in each 1 kb window. Inset in Figure 1F) is the high resolution coverage map (average reads per 0.2 kb window) of the relA region. The origin of replication is at the 0/4.2 Mbp genomic position, and the terminus of replication is at the 2.1 Mbp genomic position. NCIB 3610 and relA-* were grown to mid log phase, while the other strains were grown to stationary phase. The different shapes of the coverage maps might be partly due to these differences in growth phases.