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Combined Analysis of Murine and Human Microarrays and ChIP Analysis Reveals Genes Associated with the Ability of MYC To Maintain Tumorigenesis

Figure 4

mRNA expression of ribosomal structural proteins and the associated rates of protein synthesis were reduced upon MYC inactivation in osteosarcomas and lymphomas.

(A) Gene list of all the ribosomal structural proteins (total of 82 genes) were generated and applied for filtering the microarray data of MYC inactivation and reactivation time-course. 61 ribosomal structural proteins with p<0.01 in StepMiner analysis are shown on the right. (B) validation of mRNA expression of ribosomal structural proteins RPS5, RPS11, RPS21, RPS24, RPS25, RPS27, RPL3, RPL6, RPL10a, RPL24 and RPL44 by quantitative real-time PCR in osteosarcomas upon MYC inactivation and reactivation (top panel) and lymphomas upon MYC inactivation (bottom panel). (C) Rates of protein synthesis upon MYC inactivation (bone tumors and lymphomas) and reactivation (bone tumors) were assayed by measuring the S35 methione and S35 Cysteine incorporation to the protein.

Figure 4