Diversity of Flowering Responses in Wild Arabidopsis thaliana Strains
Figure 5
Newly Identified Alleles of FRI and FLC
(A) Diagrams of FRI alleles. Exons are represented by boxes, introns by lines. Black lines indicate nonsynonymous changes, gray lines synonymous changes as well as polymorphisms in the non-coding region compared to the reference sequence of H51 [4]. Insertions and deletions (indels) are designated by triangles, numbers indicate size in base pairs (bp). Premature stop codons are caused by a 1-bp insertion in Ang-1, single bp polymorphisms in BG1 and Ri-0, or a deletion and inversion (gray box) in Pog-0. Note extensive polymorphisms in Jl-1. BG4, BG6, BG9, and HR5 were only partially sequenced.
(B) Changes in FLC transcripts in three accessions. Dotted lines indicate exons that are missing in part or completely. These deletions cause frame shifts and thus altered amino acid sequences (grey boxes). Premature stop codons are indicated. The reasons for the aberrant transcript processing in Ll-2 are not known. In Cen-0, an alternative splice acceptor site in the last exon is used, leading to a deletion of exon sequences and a frame shift, whereas in Cal-0 an alternative splice acceptor site in the last intron is used, which adds additional sequences and also causes a frame shift of sequences of the last exon.
(C) Large insertions in the first intron of FLC in several accessions.