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H1 Histones, Thymine and Non-Histone Chromosomal Proteins Direct Cell Differentiation

Posted by jamesmhoward on 22 May 2012 at 11:37 GMT

It is my hypothesis of 1979 that H1 histones organize DNA by links to the free carbonyl of thymine which is located in the minor groove of DNA. Furthermore, I suggest that "non-histone chromosomal proteins (NHCPs)," what they were called in 1979, act to stimulate transcription by complexing with the H1 histones to remove them from DNA. Therefore, the DNA is then available for transcription by pertinent enzymes.

When H1 histones are attached to DNA, transcription does not occur. When NHCPs remove the H1s, then transcription occurs. The specificity of this mechanism is induced by the placement of thymine bases. Therefore, H1 histones act to close chromatin in a non-specific manner. When "specific" NHCPs arrive, the H1 histones are removed in a specific manner which involves the specific placement of thymine bases and the specificity of the NHCPs. ...a very specific combination. ("Transcription - A Hypothetical Model 1979," at: )

My explanation of transcription control relates to Zhang, et al., in the following manner:

The placement of thymine bases and H1 histones establish the basis of "pluripotent" chromatin. That is, the thymine - H1 complexes are available for a cascade of NHCPs to remove the H1 histones in a specific, temporal sequence. Without the H1 - thymine connections, the NHCPs cannot induce this specific, temporal wave of transcription which directs the potential pathway of a pluripotent cell. This specific, temporal removal of H1 histones, and subsequent removal of other H1 histones from other areas of thymine within the DNA by subsequent, different NHCPs determines cell differentiation.

No competing interests declared.