TY - JOUR T1 - The DNA Replication Factor RFC1 Is Required for Interference-Sensitive Meiotic Crossovers in Arabidopsis thaliana A1 - Wang, Yingxiang A1 - Cheng, Zhihao A1 - Huang, Jiyue A1 - Shi, Qian A1 - Hong, Yue A1 - Copenhaver, Gregory P. A1 - Gong, Zhizhong A1 - Ma, Hong Y1 - 2012/11/08 N2 - Author Summary Meiotic recombination is important for pairing and sustained association of homologous chromosomes (homologs), thereby ensuring proper homolog segregation and normal fertility. DNA synthesis is thought to be required for meiotic recombination, but few genes coding for DNA synthesis factors have been studied for possible meiotic functions because their essential roles in the mitotic cell cycle make it difficult to study their meiotic functions due to the lethality of corresponding null mutations. Current models for meiotic recombination only include leading strand DNA synthesis. We found that the Arabidopsis gene encoding the DNA REPLICATION FACTOR C1 (RFC1) important for lagging strand synthesis promotes meiotic recombination via a specific pathway for crossovers (COs) that involves the formation of double Holliday Junction (dHJ) intermediates. Therefore, lagging strand DNA synthesis is likely important for meiotic recombination. Because DNA synthesis is a highly conserved process and meiotic recombination is highly similar among budding yeast, mammals, and flowering plants, the proposed function of lagging strand synthesis for meiotic recombination might be a general feature of meiosis. JF - PLOS Genetics JA - PLOS Genetics VL - 8 IS - 11 UR - https://doi.org/10.1371/journal.pgen.1003039 SP - e1003039 EP - PB - Public Library of Science M3 - doi:10.1371/journal.pgen.1003039 ER -