TY - JOUR T1 - Steroid Hormone Control of Cell Death and Cell Survival: Molecular Insights Using RNAi A1 - Chittaranjan, Suganthi A1 - McConechy, Melissa A1 - Hou, Ying-Chen Claire A1 - Freeman, J. Douglas A1 - DeVorkin, Lindsay A1 - Gorski, Sharon M. Y1 - 2009/02/13 N2 -
Hormones regulate complex signaling pathways required for the differentiation, growth, survival, and death of cells in diverse organisms. The insect steroid hormone 20-hydroxy ecdysone (ecdysone) triggers cell death of obsolete larval tissues, such as the midgut and salivary glands, during metamorphosis and provides a model system for understanding steroid hormone control of cell death and cell survival. Previous studies identified many genes and proteins associated with fruit fly salivary gland cell death, but functional verification was lacking. Here, we have analyzed 460 of those genes using RNAi, a genetic approach to inhibit gene function, to assess their possible cell death or cell survival related function. To our knowledge, this is the first large-scale functional screen for genes involved in steroid hormone regulated cell death and cell survival. We identified several novel ecdysone regulated components with a cell death/survival role, including genes with no previously known function.