TY - JOUR T1 - Nanoliter Reactors Improve Multiple Displacement Amplification of Genomes from Single Cells A1 - Marcy, Yann A1 - Ishoey, Thomas A1 - Lasken, Roger S A1 - Stockwell, Timothy B A1 - Walenz, Brian P A1 - Halpern, Aaron L A1 - Beeson, Karen Y A1 - Goldberg, Susanne M. D A1 - Quake, Stephen R Y1 - 2007/09/21 N2 -
It is often challenging to manipulate or analyze the genetic material or genome of an individual cell. Biochemical DNA amplification technologies can be used to make many copies of the genome from a single cell, and in this paper we investigated how well such amplification works as a function of the reaction volume. We found that single-cell genome amplification in nanoliter volumes is much more effective than in microliter volumes, providing better representation of the starting genome with less bias in the product. It should therefore be possible to obtain high-quality genome sequences from single cells. This is useful because very few microbes can be obtained in pure culture, and are therefore only amenable to single-cell analysis.