TY - JOUR T1 - Three Essential Ribonucleases—RNase Y, J1, and III—Control the Abundance of a Majority of Bacillus subtilis mRNAs A1 - Durand, Sylvain A1 - Gilet, Laetitia A1 - Bessières, Philippe A1 - Nicolas, Pierre A1 - Condon, Ciarán Y1 - 2012/03/08 N2 - Author Summary RNA turnover is an important way of controlling gene expression. While the characterization of the pathways and enzymes for RNA degradation are well-advanced in Escherichia coli and yeast, studies in Gram-positive bacteria have lagged behind. This tiling array study shows that two essential enzymes, the single-strand specific endonuclease RNase Y and the 5′–3′ exoribonuclease RNase J1, play central roles in the degradation of mRNAs in Bacillus subtilis. The double-strand specific enzyme RNase III plays a more minor role in RNA turnover, but has significant indirect effects on transcription. Depleting cells of these key enzymes led to the stabilization of many potentially regulatory RNAs, which were otherwise revealed only through testing a wide variety of experimental conditions. It is now possible to tell at a glance which of these three RNases is involved in the turnover of your favorite mRNA. JF - PLOS Genetics JA - PLOS Genetics VL - 8 IS - 3 UR - https://doi.org/10.1371/journal.pgen.1002520 SP - e1002520 EP - PB - Public Library of Science M3 - doi:10.1371/journal.pgen.1002520 ER -