Inducible and Reversible Clock Gene Expression in Brain Using the tTA System for the Study of Circadian Behavior

Hee-Kyung Hong; Jason L Chong; Weimin Song; Eun Joo Song; Amira A Jyawook; Andrew C Schook; Caroline H Ko; Joseph S Takahashi

doi:10.1371/journal.pgen.0030033

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Open Access

Peer-reviewed

Research Article

Inducible and Reversible Clock Gene Expression in Brain Using the tTA System for the Study of Circadian Behavior

Hee-Kyung Hong,

Affiliations Howard Hughes Medical Institute, Northwestern University, Evanston, Illinois, United States of America , Center for Functional Genomics, Northwestern University, Evanston, Illinois, United States of America , Department of Neurobiology and Physiology, Northwestern University, Evanston, Illinois, United States of America
⨯
Jason L Chong,

Affiliation Department of Neurobiology and Physiology, Northwestern University, Evanston, Illinois, United States of America
⨯
Weimin Song,

Affiliations Howard Hughes Medical Institute, Northwestern University, Evanston, Illinois, United States of America , Department of Neurobiology and Physiology, Northwestern University, Evanston, Illinois, United States of America
⨯
Eun Joo Song,

Affiliations Howard Hughes Medical Institute, Northwestern University, Evanston, Illinois, United States of America , Department of Neurobiology and Physiology, Northwestern University, Evanston, Illinois, United States of America
⨯
Amira A Jyawook,

Affiliations Center for Functional Genomics, Northwestern University, Evanston, Illinois, United States of America , Department of Neurobiology and Physiology, Northwestern University, Evanston, Illinois, United States of America
⨯
Andrew C Schook,

Affiliations Howard Hughes Medical Institute, Northwestern University, Evanston, Illinois, United States of America , Department of Neurobiology and Physiology, Northwestern University, Evanston, Illinois, United States of America
⨯
Caroline H Ko,

Affiliations Department of Neurobiology and Physiology, Northwestern University, Evanston, Illinois, United States of America , Department of Psychology, University of Toronto, Toronto, Ontario, Canada
⨯
Joseph S Takahashi

*To whom correspondence should be addressed. E-mail: j-takahashi@northwestern.edu

Affiliations Howard Hughes Medical Institute, Northwestern University, Evanston, Illinois, United States of America , Center for Functional Genomics, Northwestern University, Evanston, Illinois, United States of America , Department of Neurobiology and Physiology, Northwestern University, Evanston, Illinois, United States of America
⨯

Inducible and Reversible Clock Gene Expression in Brain Using the tTA System for the Study of Circadian Behavior

Hee-Kyung Hong,
Jason L Chong,
Weimin Song,
Eun Joo Song,
Amira A Jyawook,
Andrew C Schook,
Caroline H Ko,
Joseph S Takahashi

Published: February 23, 2007
https://doi.org/10.1371/journal.pgen.0030033

About the Authors

Hee-Kyung Hong

Affiliations Howard Hughes Medical Institute, Northwestern University, Evanston, Illinois, United States of America , Center for Functional Genomics, Northwestern University, Evanston, Illinois, United States of America , Department of Neurobiology and Physiology, Northwestern University, Evanston, Illinois, United States of America

Jason L Chong

Affiliation Department of Neurobiology and Physiology, Northwestern University, Evanston, Illinois, United States of America

Weimin Song

Affiliations Howard Hughes Medical Institute, Northwestern University, Evanston, Illinois, United States of America , Department of Neurobiology and Physiology, Northwestern University, Evanston, Illinois, United States of America

Eun Joo Song

Amira A Jyawook

Affiliations Center for Functional Genomics, Northwestern University, Evanston, Illinois, United States of America , Department of Neurobiology and Physiology, Northwestern University, Evanston, Illinois, United States of America

Andrew C Schook

Caroline H Ko

Affiliations Department of Neurobiology and Physiology, Northwestern University, Evanston, Illinois, United States of America , Department of Psychology, University of Toronto, Toronto, Ontario, Canada

Joseph S Takahashi

*To whom correspondence should be addressed. E-mail: j-takahashi@northwestern.edu

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

HKH, JLC, and JST conceived and designed the experiments. HKH, JLC, WS, EJS, AAJ, ACS, and CHK performed the experiments. HKH analyzed the data and contributed reagents/materials/analysis tools. HKH and JST wrote the paper.

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Genetically modified animals
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Circadian rhythms
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Mouse models
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Circadian oscillators
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Light pulses
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Chronobiology
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Animal behavior
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Gene expression
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