Automatically tracking neurons in a moving and deforming brain
Neural activity traces from 156 neurons in the brain a C. elegans as it freely moves on an agarose plate for 8 minutes (strain AML32). The neural activity is expressed as a fold change over baseline of the ratio of GCaMP6s to RFP for each neuron. The behavior is indicated in the ethogram. On the right is the locations of all of the detected neurons (the head of the worm is towards the top of the page). The neurons that have significant correlation with reverse locomotion are indicated in red. White gaps indicate instances where neurons failed to segment. This is a newly acquired recording, different from that in Fig 6.