Automatically tracking neurons in a moving and deforming brain
In volume 735, neuron #111 is found successfully and is indicated in green. In volume 736, however, the neuron is misidentified, shown in red. During the correction phase, all other time points vote for what the position of neuron #111 should based on a thin-plate spline deformation. A sample of votes are shown (blue ‘x’). Since the initial estimate of the position is far from the majority of consensus votes, a corrected position is assigned to be the centroid of the votes weighted by image intensity. This process is repeated to correct any errors for every neuron at every time.