Automatically tracking neurons in a moving and deforming brain
(A) Centerlines are detected from the low magnification dark field images. The centerline is shown in green and the tip of the worm’s head is indicated by a blue dot. (B) The centerline found from the low magnification image is overlaid on the high magnification RFP images. The lines normal to the centerline, shown in blue, are used to straighten the image. All scale bars are 100 μm. (C) A maximum intensity projection of the straightened volume is shown. Individual neuronal nuclei are shown (D) before and (E) after segmentation.