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Automatically tracking neurons in a moving and deforming brain

Fig 1

Schematic of analysis pipeline to segment and track neurons through time and extract their neural activity in a deforming brain.

Neurons are labeled with calcium insensitive red fluorescent proteins, RFP, and calcium sensitive green fluorescent proteins, GCaMP. Videos of the animal’s behavior and volumetric fluorescent images of the animal’s brain serve as input to the pipeline. The algorithm detects all neurons in the head and produces tracks of the neural activity across time as the animal moves.

Fig 1

doi: https://doi.org/10.1371/journal.pcbi.1005517.g001