A computational analysis of in vivo VEGFR activation by multiple co-expressed ligands
(A) VEGF isoform-specific NRP1-binding properties result in isoform-specific trafficking of VEGFR2. (B) Subcellular location-specific dephosphorylation rates for Y1175 and Y1214 (S9 Table) lead to preferential activation of tyrosine 1214 on the EC surface, compared to signaling in endosomes. (C) Isoform-specific trafficking and location-specific dephosphorylation combine to result in isoform-specific trends in relative activation of VEGFR2 on tyrosine 1175 and tyrosine 1214. (D) Total VEGFR2 phosphorylation, on at least one tyrosine (pR2) and specifically on Y1175 or Y1214, across all subcellular locations. (E-F) Distribution of pY1175 (E) and pY1214 (F), by VEGF isoform and location.