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A Biophysical Model of CRISPR/Cas9 Activity for Rational Design of Genome Editing and Gene Regulation

Fig 7

Rational design of genome editing and gene regulation.

(A) The dynamics of Cas9-based cleavage at DNA sites with either (blue) zero, (green) one, (red) two, or (cyan) three mismatches, comparing the effects of increasing guide RNA concentration by 10-fold, increasing the genome size by 2-fold, or increasing the cellular growth rate by 2-fold. (B) A sensitivity analysis shows how changing system parameters affect a DNA site’s steady-state cleavage efficiency in growing cells. (C) The dynamics of dCas9-based transcriptional repression (promoter activity) at DNA sites with either (blue) zero, (green) one, (red) two, or (cyan) three mismatches, performing the same comparisons as in A. (D) A sensitivity analysis shows how changing system parameters affect a DNA sites’ steady-state transcriptional repression (promoter activity) in growing cells. mm, mismatch.

Fig 7

doi: https://doi.org/10.1371/journal.pcbi.1004724.g007