Steering Evolution with Sequential Therapy to Prevent the Emergence of Bacterial Antibiotic Resistance
The evolutionary graphs for the fitness landscapes of E. coli with the antibiotics (a) Ampicillin, (b) Ampicillin + Sulbactam and (c) Cefprozil for 4 possible substitutions found in blaTEM-50. Arrows represent fitness–conferring mutations which can fix under the Strong Selection Weak Mutation assumptions. The absence of an arrow in either direction corresponds to a neutral mutation which cannot fix under these assumptions. Each genotype is shaded according to its fitness normalized to a 0–1 scale.