Rigidity Emerges during Antibody Evolution in Three Distinct Antibody Systems: Evidence from QSFR Analysis of Fab Fragments
Fig 7
Relating flexibility differences to changes in the H-bond network.
(A) and (B) are differences of hydrogen bonds for anti-fluorescein antibody, respectively. (C) and (D) for anti-CD3 antibody. (E) and (F) for esterase catalytic antibody. Regions that exhibit significant increased rigidity/flexibility are affected by gain/loss local H-bonds. Large increases in rigidity due to gain of H-bonds are displayed in the left panel while large increases in flexibility due to loss of H-bonds are in the right panel. The middle panel shows the same as in Fig 5. Hydrogen bonds are highlighted in yellow dashed lines.