Thermal Stabilization of Dihydrofolate Reductase Using Monte Carlo Unfolding Simulations and Its Functional Consequences
Fig 7
Correlation between the relative simulated and experimental Tm values.
(A) Plot of simulated Tm vs. experimental Tm. The relative Tm values were calculated by normalizing to WT: (Tm(mutant)-Tm(wild type))/ Tm(wild type). Experimental values from this study and from Bershtein et al. [48] are included. WT is shown as a blue triangle. r = 0.65, p = 3 x 10−6. (B) Plot of simulated Tm vs. experimental Cm. r = 0.68. p = 6 x 10−7.