Laminar and Dorsoventral Molecular Organization of the Medial Entorhinal Cortex Revealed by Large-scale Anatomical Analysis of Gene Expression
(A) Boxes indicate the dorsal (orange) and ventral (red) regions from which mean pixel intensities (mINT) were extracted for identification of genes defining the dorsal border of the MEC. (B) Example cropped ISH images downloaded from the ABA API are shown (left) adjacent to mean expression pattern for genes expressed in MEC but not the wedge-shaped region dorsal to MEC (right). Arrows indicate regional borders. (C) Raw ISH images show the dorsal border of MEC layer II marked out by the gene Nov at different medio-lateral positions (left) and in the coronal plane (right). Values in mm indicate distance from Bregma. (D) Example cropped ISH images downloaded from the ABA API are shown for genes expressed in the wedge-shaped region dorsal to MEC but not the most dorsal MEC region. (E) Raw ISH sagittal (Sag) and coronal (Cor) images from the ABA indicate the continuity of the wedge-shaped region with the more medial parasubicular region for the genes Igfbp6 and Kctd16. (F) Boxes highlighting regions used to identify the ventral border of the MEC. See (A). (G-H) Example cropped ISH images downloaded from the ABA API (see Materials and Methods) are shown adjacent to images of the mean expression pattern for genes expressed in MEC but not the more ventral region (G), or in the ventral region but not MEC (H).