Phase Transitions in the Multi-cellular Regulatory Behavior of Pancreatic Islet Excitability
Figure 3
Link between phase transitions in [Ca2+]i and physiological parameters.
A) Percent cells showing [Ca2+]i elevations averaged over islets from each Kir6.2[ΔN30,K185Q]-expressing mouse as a function of Pexc (100%-%GFP). Right: Mean(±s.e.m.) for data binned to wild-type, pre- and post-critical ranges as determined by %GFP. *indicates significant difference (p<0.0001) between data as indicated. B) Plasma insulin levels from each mouse as a function of Pexc. Right: Mean(±s.e.m.) for data binned as in A. *indicates significant difference (p<0.05) between data as indicated. C) Time-averaged blood glucose levels from each mouse as a function of Pexc. Right: Mean(±s.e.m.) for data binned as in A. *indicates significant difference (p<0.0001) between data as indicated. D) Insulin secretion from isolated islets at 20 mM glucose (left) and 2 mM glucose (right), averaged over each mouse as a function of Pexc. E) Mean(±s.e.m.) for data in D binned as in A. *indicates significant difference (p<0.01) between data, ‘ns’ indicates no significant difference (p>0.05) as indicated. F) Mean(±s.e.m.) of islet insulin content averaged over each mouse. Solid lines in A,B,D represent Boolean model fit (p = 0.3) from experimental data in Figure 2C.