Identification of Key Hinge Residues Important for Nucleotide-Dependent Allostery in E. coli Hsp70/DnaK
Figure 2
(A) The first eigenvector of NBD essential dynamics (ED) involves a shearing motion of the domains I and II. Left – side view of the NBD, in which ED vectors of domains I and II are colored blue and red, respectively. Right – front view of the NBD. The shearing motion of domains I and II is manifested through the two helices at the interface of subdomains I-A and II-A, as indicated in the circle. (B) Second eigenvector of NBD ED involves a rotating motion of primarily subdomain II-B, which may change the distance between subdomains I-B and II-B. Subdomain II-B is colored red, and the other three subdomains are in blue.