The Interaction of Vinculin with Actin
Figure 11
The open II conformation allows for dynamic linking to F-actin.
The interaction of talin VBS with D1 of vinculin is regulated by the conformation of vinculin. (I) In its closed conformation vinculin can only link to talin VBS. (II) Transition of vinculin form a closed conformation to an open I conformation would result form interaction of Vt with F-actin combined with partial linking of D1 to talin. (III) Formation of the open I conformation allows for complete talin VBS insertion in to D1. (IV) Following formation of open I vinculin, further forces from F-actin can cause formation of the open II conformation. With the open II conformation vinculin can form a dynamic link to F-actin.