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Standard Anatomical and Visual Space for the Mouse Retina: Computational Reconstruction and Transformation of Flattened Retinae with the Retistruct Package

Figure 6

Alignment of the binocular zone in visuotopic coordinates. A,

Azimuthal equilateral projections in standard retinal space of left and right retinae with ipsilateral (upper) and contralateral (lower) label resulting from bilateral injections of Fluoro-Ruby (magenta) and Fluoro-Emerald (cyan) into left and right dLGN, respectively, of the same mouse. Plots were generated from stitched 10× epifluorescent images and cell locations detected using ImageJ. For this figure, we have abandoned the convention of always plotting nasal retina to the right. B, Schematic illustrating the approximate projection of retinal space onto visual space. When the orientation of the optic axis (grey line) is optimal, the ipsilateral crescent is projected entirely to the opposite visual field. Note that due to the refraction in the lens the visual field is estimated to be 180° for each eye. C–D, Orthographic projections in central visuotopic space of the two ipsilateral retinae in A with optic axis (*) at 64° azimuth; 22° elevation (C) and with optic disc at 60° azimuth; 35° elevation (D). E, Sinusoidal projection of contralateral retinae in B with the optic axis (*) at 64° azimuth; 22° elevation. Labels N, D, T, V indicate the projection of the corresponding pole of the retina. Grid spacing is 15°.

Figure 6