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Climbing Fiber Burst Size and Olivary Sub-threshold Oscillations in a Network Setting

Figure 4

Effects of network synchrony on AP spikelet count.

A. Phase dependency of AP spikelet count for different network sizes when a network consists of coupled cells with near-synchronized STOs (STO amplitude >5.5 mV, top row) or non-synchronized STOs (STO amplitude <5.5 mV, bottom row). Phase ranges where no spikes were fired are indicated in red. Top row, near synchrony - The small 1×3 network shows a weak phase dependency of the AP spikelet count, averaging 0.5 spikelets on the upward slope of the STO and 2 spikelets around the peak. The 3×3 and 5×5 networks show similar outcomes and a stronger phase-dependency of the AP spikelet count, averaging 0 spikelets on the upward slope, followed by 1 spikelet near the peak and 3 spikelets around the peak. Due to a strong depolarizing current (5 pA, 20 ms for 1×3 networks and 6 pA, 20 ms for 3×3 and 5×5 networks, see Methods), spikes are sometimes fired outside the usual firing window in the trough of the oscillation (1.25π radians bin, total of 7 occurrences in 2100 simulations across all network sizes). Bottom row, no synchrony - Regardless of network size, there is no clear phase-dependency of AP spikelet count. A rounded average of 2 spikelets is seen across all phases, except for part of the phase range falling outside or close to the bounds of the firing window where a rounded average of 1 spikelet may occur. Due to analysis restrictions imposed by determining the phase, STO amplitudes smaller than 1 mV are poorly represented in the data set. B. AP spikelet distributions across STO amplitudes for different network sizes. Warmer colors denote more occurrences, cooler colors less. The distribution of spikelets changes as a function of STO amplitude. In a range of approximately 5 to 7 mV, the distribution broadens, corresponding with the phase dependency of AP spikelet counts shown in panel A. At an STO amplitude of 7.5 mV or more, this distribution narrows again, but at a lower average spikelet count than was seen at lower amplitudes. The average number of AP spikelets shows a downward trend for all network sizes, as illustrated by the fitted trend lines shown in white.

Figure 4