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Weakly Circadian Cells Improve Resynchrony

Figure 1

TTX-treatment and wash-out in SCN explants reveals differences in the dynamics of desynchrony and resynchrony of single cells.

(A) Raster plot of bioluminescence (a. u.) from single neurons in an organotypic SCN explant before (days 1–6), during (days 7–12), and following treatment with 0.5 µM tetrodotoxin (TTX, days 13–18). Time of TTX treatment (days 7–12) is indicated by the shaded region of the bar along the top of the raster plot. TTX minimizes cell-cell communication and reveals intrinsic circadian properties. Here we plot traces from 123 neurons that show a range of oscillatory behaviors in TTX, all of which are rhythmic when coupling is in place before treatment. Following washout, rhythms return to all cells, but at different rates. The intensity of the white is proportional to the bioluminescence in each experimental condition. (B) Preliminary data suggests a small population of neurons resynchronizes first and then a slow recruitment of the remaining population is observed following TTX washout. Here we include the SI for the entire population (black), as well as the SI for each cohort of cells. Cohorts are determined by when cells become rhythmic after the wash; cells in cohort 1 (blue) become rhythmic first (hours 31–35), cells in cohort 2 (green) become rhythmic second (hours 36–45), and cells in cohort 3 (red) become rhythmic after hour 46 of the wash. (C) The location of single cells recorded in the SCN explant. Cell position is color-coded based on the resynchronization cohort.

Figure 1

doi: https://doi.org/10.1371/journal.pcbi.1002787.g001