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Microbial Co-occurrence Relationships in the Human Microbiome

Figure 1

Methodology for characterizing microbial interactions using a compendium of similarity measures.

16S data from the Human Microbiome Project (HMP) were collected from 18 body sites in a cohort of 239 healthy subjects and assessed using 16S rRNA gene sequencing. We analyzed microbial co-occurrence and co-exclusion patterns in these data by developing two complementary approaches: a compendium of Generalized Boosted Linear Model (GBLMs) and an ensemble of similarity and dissimilarity measures. Each approach produced a network in which each node represented a microbial taxon within one body site, and each edge represented a significant association between microbial or whole clade abundances within or across body sites. The resulting association networks produced by each individual method were merged as p-values using Simes method, after which FDR correction was performed. Associations with FDR q-values>0.05, inconclusive directionality, or fewer than two supporting pieces of evidence were removed. This provided a single global microbial association network for taxa throughout the healthy commensal microbiota.

Figure 1