Adhesion Failures Determine the Pattern of Choroidal Neovascularization in the Eye: A Computer Simulation Study
A) Total number of stalk cells vs. time. B) Total number of stalk cells confined in the sub-RPE space vs. time. C) Total number of stalk cells in contact with the POS (stalk cells in the sub-retinal space) vs. time. D) Total number of RPE cells vs. time. E) Total contact area between RPE cells and BrM vs. time. F) Total contact area between POS cells and BrM vs. time. The different colors represent the results of 10 simulation replicas of the adhesion scenario (RRl = 1, RRp = 1, RBl = 2, RBp = 2, ROl = 3) (Table S10, adhesion scenario ID: 53). (A, B, C) CNV initiates in all replicas and all replicas develop ET3 CNV. During the first month after initiation, stalk cells gradually invade both the sub-RPE space and the sub-retinal space, with more invading the sub-RPE space. Between months 1 and 2 about 30% of the sub-RPE stalk cells transmigrate into the sub-retinal space. After month 3, the number of sub-RPE stalk cells increases slowly, while the number of sub-retinal stalk cells remains constant. (E) During the first month of the simulation, the contact area between the RPE and BrM rapidly decreases as stalk cells invade the sub-RPE space. Between months 1 and 2, the contact area between the RPE and BrM rapidly increases as sub-RPE stalk cells transmigrate into the sub-retinal space. The contact area between the RPE and BrM slowly decreases after month 3 throughout the simulated year. (D) A few RPE cells die in most replicas. (F) In a few replicas the POS persistently contacts BrM, as the RPE develops small holes.