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Ligand-Induced Modulation of the Free-Energy Landscape of G Protein-Coupled Receptors Explored by Adaptive Biasing Techniques

Figure 1

Free-energy of the unliganded receptor.

(A) Free-energy as a function of the position (s) along the activation pathway. Note that the curve has been shifted so that the lowest energy minima (indicated by stars) correspond to a reference free-energy value. (B) Free-energy as a function of ionic lock distance (dIL) and the toggle switch dihedral (χTS) molecular switches for the unliganded receptor; contour lines are reported every kBT.

Figure 1

doi: https://doi.org/10.1371/journal.pcbi.1002193.g001