Evaluating Gene Expression Dynamics Using Pairwise RNA FISH Data

doi:10.1371/journal.pcbi.1000979

Evaluating Gene Expression Dynamics Using Pairwise RNA FISH Data

Figure 2

Maximum likelihood estimation (MLE) applied to synthetic FISH data in the continuous regime.

(A) Comparison of true mRNA dynamics (red curve) with MLE reconstructions based on 4 genes (solid blue curve) and 2 genes (dashed blue curve), for observations per gene pair. The data points are the actual synthetic data used for genes 1 and 2 in both cases. The maximum noise amplitude used to generate the data is . (B) The reconstruction error , averaged over 20 realizations of parameters with , steadily decreases as the number of genes and the number of FISH observations per gene pair are increased.

doi: https://doi.org/10.1371/journal.pcbi.1000979.g002