Adaptable Functionality of Transcriptional Feedback in Bacterial Two-Component Systems
A. Dynamic response of downstream (DS) protein expression for Circuit I (feedback to RR alone) is slower than the wild-type architecture. denotes time to attain 95% of the activated steady state of downstream protein (with kph = 0.1) in the system with constitutive expression of the shk gene, normalized to the same system with no feedback. is the same ratio for wild-type system with both SHK and RR under feedback regulation. B. Steady state dose-response shows reduced induction range for in Circuit II (feedback to SHK alone) as compared to the wild-type (single operon) case. Induction range denotes steady-state difference between phospho-RR dimer with kph = 0 to find the highest, saturated level, and with kph = 10 (basal expression) in the wild-type (wt) case and the case with constitutive expression of the rr gene, again normalized to the same system with no feedback. Insets show kinetics and dose-response of reference parameter set (Table 2; open circles on scatter plots). Constitutive gene expression is set to equal feedback-regulated expression at high signal, kph = 0.1.