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Global Motions of the Nuclear Pore Complex: Insights from Elastic Network Models

Figure 5

First two modes of yeast NPC.

Three views of the two slowest modes of the NPC without (A and D) and with (B and E) the NE fragment are shown. The z-axis is shown in panel A, and orientations are the same in panels B, D and E. Mode 1 is a “stretching/elongation” mode that mobilizes the nuclear face (z<0) more than the cytoplasmic face, whether or not the NE fragment is included in the model. Mode 2 is a “bending” mode that elongates the NPC in the absence of the NE (D), but not when the NE is included (E). For a clearer visualization of the motion, the lower half of the structure is shown in translucid colors in panel E. Scaled mobilities are plotted against axial position in panels C and F for the NPC alone (blue) and the NPC/NE (red). The addition of the NE decreases mobility of the POM rings in the first mode, as shown by the absence of a peak near z = 0 in panel C. In the second mode, the NE creates a more even distribution of motion in the cytoplasmic and nuclear faces, as shown by the peaks near z = +/−12 for the red curve in panel F.

Figure 5