Biomedical Discovery Acceleration, with Applications to Craniofacial Development
In situ hybridization using anti-sense probes for Apobec2 (A–C), E430002G05Rik (D–F), Hoxa2 (G–I), and Zim1 (J, K). (A, D, G, J) sagittal sections of an E11.5 head; (B, C, E, F, H, I) are transverse sections of an E12.5 head. Anterior is to the right on all panels. Dark staining represents hybridization signal from the probe, the pink color is from a histological counterstain. The arrows indicate areas of fainter staining. (B, E, H) are more rostral sections than (C, F and I). The tongue has a mild convexity at these stages of development, being raised on its rostral aspect (see panels J). Therefore, more rostral sections will tend to skim the midsection of the tongue at the surface. More caudal sections will tend to intersect with staining patterns at their anterior and posterior domains (compare panels B and C). Apobec2 and E430002G05Rik did not generate significant tongue staining at E11.5. Control experiments using sense probes did not yield specific staining. 1, mandibular component of first branchial arch (future lower jaw as well as future anterior and middle of tongue); 2, second branchial arch (future posterior, lateral, part of tongue – major site of Hoxa2 expression); d, mandible; h, heart; hb, hindbrain; n, nasal prominence; ns, nasal septum; t, tongue; x, maxillary process.