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Modeling Recursive RNA Interference

Figure 9

Modeling transient transfection experiments.

(A) reporter gene expression levels with and without recursive RNAi of Dicer in which the source of dsRNA decays with first order kinetics to represent dilution of plasmids by cell division, with decay constant δ. All simulations run with γ = 10. (Blue, green, light blue) targeting of reporter only without targeting of RNAi components. (Red, orange, yellow) targeting of Dicer along with the reporter. Decay of dsRNA source leads to transient reduction in reporter that restores at a rate dependent on decay rate of source. Rapid decay of dsRNA source leads to less difference between recursive and non-recursive RNAi (compare δ = 1, where almost no difference is seen when Dicer is also targeted, with a tenfold slower decay rate δ = 0.1, where Dicer targeting clearly increases expression level during transient knockdown). (B) Dependence of restoration on RNAi efficacy parameter γ for different rates of dsRNA source (plasmid) decay.

Figure 9