Reader Comments

Post a new comment on this article

on the possible sequencing error

Posted by gplunkett3 on 01 Jun 2012 at 19:44 GMT

We have noticed that the gene sequence of ECK0512/ylbE presents a frameshift which is absent in all other sequenced E. coli strains and may be due, according to UniProt, to a sequencing error.

While the pseudogene ylbE was thought to be a domestication mutant allele, resequencing data does seem to support the possibility of a sequencing error. See:

Nahku R, Peebo K, Valgepea K, Barrick JE, Adamberg K, Vilu R (2011) Stock culture heterogeneity rather than new mutational variation complicates short-term cell physiology studies of Escherichia coli K-12 MG1655 in continuous culture. Microbiology 157(Pt 9):2604-2610 [PMID: 21700661]

Freddolino PL, Amini S, Tavazoie S (2012) Newly identified genetic variations in common Escherichia coli MG1655 stock cultures. J Bacteriol 194(2):303-306 [PMID: 22081388]

No competing interests declared.