Revising Your Manuscript

Submission Instructions

When you revise your manuscript, be prepared to provide the materials listed on this page as part of the revision process. 

The journal performs technical checks when you resubmit your manuscript to ensure that it meets our formatting and publishing requirements.

We recommend that you refer to our checklist of formatting requirements before resubmitting in order to expedite your manuscript through the technical checks. Download the formatting checklist (PDF).

Response to Reviewers

Your response to reviewers should address the specific points made by each reviewer. Include your responses to all the reviewers’ and editors’ comments and list the changes you have made to the manuscript.

  Help uploading your response to reviewers
  • If you are working on a revision in Aperta: You can choose whether to upload your response to reviewers in a separate file or enter your comments directly in the submission system on the Response to Reviewers card.

  • If you are working on a revision in Editorial Manager: Upload your response as a “Response to Reviewers” file in the “Attach Files” section of the system.

Revised Manuscript File

Provide a clean review copy of your revised manuscript file as well as a tracked changes version that shows the changes you have made since the original submission. 

Note: Your figure files, supporting information files, and other information provided in your original submission will automatically transfer to your revised submission unless you choose to update and replace them. 
  Help uploading your revised manuscript files
  • If you are working on a revision in Aperta: Upload the clean copy of your revised manuscript to the Upload Manuscript card. Upload the tracked changes version of the manuscript to the Response to Reviewers card.

  • If you are working on a revision in Editorial Manager: Upload the clean copy of your revised manuscript as the “Manuscript” file. Upload the tracked changes version of the manuscript as a “Related Manuscript” file.

Author Summary

The Author Summary is a 150-200 word non-technical summary of the work. Subject to editorial review, this short text is published with all research articles as a highlighted text box. 


Weinberger LS, Shenk T (2007) An HIV Feedback Resistor: Auto-Regulatory Circuit Deactivator and Noise Buffer. PLoS Biol 5(1): e9. doi:10.1371/journal.pbio.0050009

Kim Guisbert KS, Li H, Guthrie C (2007) Alternative 3′ Pre-mRNA Processing in Saccharomyces cerevisiae Is Modulated by Nab4/Hrp1 In Vivo. PLoS Biol 5(1): e6. doi:10.1371/journal.pbio.0050006

Distinct from the scientific abstract, the Author Summary is included in the article to make findings accessible to an audience of both scientists and non-scientists. Ideally aimed to a level of understanding of an undergraduate student, the significance of the work should be presented simply, objectively, and without exaggeration.

Authors should avoid the use of acronyms and complex scientific terms and write the text using a first-person voice. Authors may benefit from consulting with a science writer or press officer to ensure they effectively communicate their findings to a general audience. 

  Help uploading your Author Summary
  • If you are working on a revision in Aperta: Include the Author Summary in your revised manuscript file after the Abstract and before the Introduction.

  • If you are working on a revision in Editorial Manager: Provide your Author Summary as a separate file and upload it as a “Related Manuscript” file with the title “Author Summary.” 


The Blurb is a brief statement about the work that is about 20-30 words (1-2 sentences) long. Note that the blurb is not published with the manuscript. It is used in our weekly and monthly Electronic Table of Contents notifications, sent out to users signed up to receive alerts from PLOS Biology, and on the journal homepage.


During embryonic development of the motor system of Drosophila, motorneurons target their dendrites to different regions along the body axis in response to midline guidance cues.

A neuroimaging study reveals novel insights into how the brain responds to an anticipated event, such as a starting gun or responding to a green light.

Computational modeling and experimentation in a model system for actin-based force generation explain how actin networks initiate and maintain directional movement.


For further examples, please view the blurbs accompanying the articles on the homepage of PLOS Biology.

The blurb is subject to editorial changes. It should, without exaggeration, entice people to read your manuscript. It should not be redundant with the title and should not contain acronyms or abbreviations. 

  Help uploading your Blurb
  • If you are working on a revision in Aperta: Upload the Blurb as a separate file on the Supporting Information card. Make sure to indicate in the system that the file is not for publication.

  • If you are working on a revision in Editorial Manager: Include the Blurb at the bottom of the Author Summary document under the heading “Blurb.”

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