Intense reproductive competition often continues long after animals finish mating. In many species, sperm from one male compete with those from others to find and fertilize oocytes. Since this competition occurs inside the female reproductive tract, she often influences the outcome through physical or chemical factors, leading to cryptic female choice. Finally, traits that help males compete with each other are sometimes harmful to females, and female countermeasures may thwart the interests of males, which can lead to an arms race between the sexes known as sexually antagonistic coevolution. New studies from Caenorhabditis nematodes suggest that males compete with each other by producing sperm that migrate aggressively and that these sperm may be more likely to win access to oocytes. However, one byproduct of this competition appears to be an increased probability that these sperm will go astray, invading the ovary, prematurely activating oocytes, and sometimes crossing basement membranes and leaving the gonad altogether. These harmful effects are sometimes observed in crosses between animals of the same species but are most easily detected in interspecies crosses, leading to dramatically lowered fitness, presumably because the competitiveness of the sperm and the associated female countermeasures are not precisely matched. This mismatch is most obvious in crosses involving individuals from androdioecious species (which have both hermaphrodites and males), as predicted by the lower levels of sperm competition these species experience. These results suggest a striking example of sexually antagonistic coevolution and dramatically expand the value of nematodes as a laboratory system for studying postcopulatory interactions.
Citation: Ellis RE, Schärer L (2014) Rogue Sperm Indicate Sexually Antagonistic Coevolution in Nematodes. PLoS Biol 12(7): e1001916. https://doi.org/10.1371/journal.pbio.1001916
Published: July 29, 2014
Copyright: © 2014 Ellis, Schärer. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: REE was supported by NSF grant 1021128, and LS was supported by Swiss NSF grant 31003A-143732. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
On the Origin of Species focused almost exclusively on the role of natural selection in evolution , but Darwin realized that animals also compete for mates and described the process of sexual selection at length in a later book . The simplest examples involve combat like that between male elephant seals fighting for access to females. However, sexual selection also includes many other types of interactions. For example, some male birds have elaborate plumage because females favor this trait when choosing mates (reviewed in ). In their simplest form, these interactions can be thought of as parts of a triangle—competition between two males forming the base and the interactions between each of the males and the female forming the two legs.
Many Types of Sexual Selection Occur after Copulation
It took almost a century for scientists to realize that sexual selection often continued after copulation was finished . If several males mate with the same female (which appears to be the rule rather than the exception), their sperm will often compete within her reproductive tract for access to her oocytes. During mating, males therefore try to enhance the success of their own sperm by actively displacing those from previous males, by chemically preventing females from further mating, or by making sperm that are competitively superior in number or phenotype (reviewed in ,). Because these interactions are played out inside the female, she will often influence and sometimes control the postcopulatory actions of both her mates and their sperm, giving her opportunities to favor the use of one male's sperm over another. This bias is often termed “cryptic female choice” and represents the postcopulatory equivalent of more familiar types of female choice (reviewed in ,).
Postcopulatory Sexual Selection Can Lead to Conflicts between Males and Females
Since male-male competition often selects for ejaculates that are more abundant or more persistent than desirable from the female's perspective, females often evolve countermeasures that control what males (or their ejaculates) can do to them, which in turn favors male traits that can overcome these female defenses. This sexual conflict between mates following insemination results in ongoing sexually antagonistic coevolution. Striking examples include manipulative seminal fluids in fruit flies , sperm with bristles in some free-living flatworms , and traumatic insemination that bypasses the normal route of fertilization in a whole range of organisms . Similar phenomena also occur during sperm-egg interactions in free-spawning marine organisms and in many cases have led to striking patterns in the evolution of the morphologies and molecules that mediate these interactions ,.
Evolutionary Biology of Caenorhabditis
Although the nematode Caenorhabditis elegans originally became famous for developmental genetics , its genus is now an important model for evolutionary research (reviewed in ). Because field studies are still difficult , most of that work is done by comparing gene functions in the laboratory, either between different wild isolates or across species. These studies have become easier with the availability of many newly discovered species, nine genome-sequencing projects (Figure 1), and the widespread availability of RNA interference  and gene-editing techniques . However, the most valuable feature is hermaphroditic genetics.
Only species with sequenced genomes are shown. Androdioecious species (comprised of males and hermaphrodites) are marked with a red symbol, and the others are dioecious (comprised of males and females). The two species in blue are able to interbreed and produce fertile offspring, and the outgroup for the elegans group is orange. Modified from Kiontke et al.  and Félix et al. . See main text for details on the types of male sperm.
C. elegans and two related species produce self-fertile, sequential hermaphrodites. Such XX individuals are female in most respects but can make their own sperm early in life and use them later for self-fertilization. The same species also make XO males, which mate with the hermaphrodites in a sexual system called androdioecy. However, males are infrequent in the wild, and outcrossing is less common than selfing in these androdioecious species ,. Since most other Caenorhabditis species are dioecious (comprised of males and females), self-fertility must have arisen on three independent occasions (Figure 1) ,,. The existence of hermaphrodites has been of great utility because it (1) simplifies genetic research in C. elegans and C. briggsae, (2) provides an important trait to follow in evolutionary studies, and (3) allows direct tests of many theories about sexual reproduction and behavior.
Sperm Competition in Nematodes
One of the best examples of such a test involves sperm competition. When C. elegans males mate with hermaphrodites, their sperm take precedence, and the hermaphrodite sperm are largely excluded from the two spermathecae (Figure 2A); this pattern of sperm precedence is due to the superiority of the male sperm themselves, rather than timing factors or seminal fluid . The advantage could be due to size, since male sperm are larger than hermaphrodite sperm in all three androdioecious species of Caenorhabditis ,. Supporting this idea, direct competition between wild isolates with different sperm sizes showed that males with larger sperm outcompete those with smaller sperm , and experimental evolution in conditions that favored multiple matings led to a rapid increase in the size of male sperm within a few dozen generations . Thus, classical studies with C. elegans provide strong evidence for sperm competition between males (Figure 2B) and for the superiority of male sperm over hermaphrodite sperm. Because C. elegans males appear to be rare in the wild, multiple matings by hermaphrodites should be uncommon, and sperm competition is probably weaker than in dioecious species.
(A) Diagram of a hermaphrodite (red) mating with a male (blue). The hermaphrodite gonad is bilaterally symmetric with a central uterus. The male is ejaculating larger sperm (blue) into the uterus, and they outcompete the smaller hermaphrodite sperm (pink) in the race to repopulate each spermatheca (purple) after sperm are dislodged during ovulation. (B) Diagram of a female (red) mating sequentially with two males (one green and the other blue). The female's gonad resembles that of the hermaphrodite in (A). The spicules from the blue male have penetrated the vulva, and he is ejaculating (blue) sperm into the uterus. These sperm will compete with those from the first male (green) for positions in the two spermathecae (purple), where they wait for the chance to fertilize oocytes. Although the sperm from the first male have already taken the best positions (shown in the right spermatheca), they will be displaced into the uterus each time an oocyte is ovulated (shown in the left spermatheca) and must compete with those from the second male to reestablish their positions. Although displacement has been directly observed, additional factors that remain unknown might help influence competition among these sperm.
Studies from C. elegans revealed additional factors that might be involved in postcopulatory sexual selection, although they have yet to be studied in an evolutionary context (Figure 3). First, oocytes are involved in attracting sperm towards the spermathecae, and their absence causes sperm to wander aimlessly . This attraction depends on a complex mixture of prostaglandins that is secreted by the oocytes . It would be fascinating to know if the spectrum of prostaglandins has changed during Caenorhabditis evolution and if any of these molecules play a role in cryptic female choice. Second, sperm release small membrane-bound packets that contain major sperm protein, which stimulates oocytes to mature and the somatic gonad to contract during ovulation ,. It is possible that these packets contain additional signals too. Thus, male and female components engage in complex physical and molecular interactions after mating, and these interactions might be under strong selective pressure.
The male ejaculates sperm (blue) and seminal fluid (green) into the uterus. The seminal fluid contains signals that activate the sperm so that they extend pseudopods and are able to crawl. The seminal fluid is probably complex and might contain additional signals. In addition, active sperm release small membrane-bound packets (blue circles) that contain major sperm protein (MSP), which stimulates oocytes to mature. MSP also causes the gonad sheath cells (orange) to contract and force oocytes through the distal spermathecal valve into the spermatheca (purple). Finally, the oocytes release a complex mixture of prostaglandins (PGs) that guide the sperm. Each of these signals has the potential to mediate sperm competition and/or cryptic female choice.
Interspecies Crosses and Postcopulatory Sexual Selection
Interspecies crosses provide a powerful technique for studying these reproductive interactions  and are used with great success in a new study by Ting et al. . Animals from a wide variety of Caenorhabditis species can easily be induced to mate with each other in the laboratory; some crosses do not result in fertilization, others lead to inviable offspring, and a few produce hybrids –. Particular attention has been focused on the androdioecious species C. briggsae and the dioecious species C. nigoni, which are so closely related that they can produce fertile offspring .
Now, Ting et al. report a remarkable discovery—in many cases when males from one Caenorhabditis species mate with hermaphrodites or females from another, they significantly decrease their fertility . This effect is caused by two distinct interactions. First, these male sperm often physically displace other sperm from the two spermathecae, as expected from previous observations of crosses between C. elegans males and hermaphrodites. Second, sperm sometimes invade the ovary, where they induce premature maturation of young oocytes and disrupt the development of new gametes. Occasionally, such ectopic sperm even cross a basement membrane and escape from the gonad altogether. The fact that C. brenneri and C. nigoni males are equally effective at displacing C. tropicalis sperm but C. brenneri males more severely affect fertility suggests that both types of interactions matter.
Some of the species in these experiments were androdioecious, whereas others were dioecious. Since sperm competition should be less intense in androdioecious species, observing how they respond in these crosses should provide a strong test for whether postcopulatory selection is involved. Indeed, the authors found that hermaphrodites were much more susceptible to harmful sperm than females. Furthermore, males from androdioecious species made the least harmful sperm detected in any of their crosses. Thus, competition between male sperm is probably at the root of the phenomena they describe.
Finally and perhaps most intriguingly, the authors found that male sperm sometimes go astray even in crosses between males and females of the same species. Thus, the interspecies crosses may simply provide a more sensitive way to measure interactions that are going on within individual species in the wild. A simple model that can explain their results is that dioecious males are under intense selection to produce highly migratory sperm, which will have the best chance to find good positions in the spermathecae for fertilizing oocytes (Figure 4A). However, the aggressiveness of these sperm means that females need to develop appropriate countermeasures, such as changes in their chemical signals or the physical strength of the distal spermathecal valve, to keep the sperm contained and prevent them from entering regions of the female gonad where they could cause harm. If the competitiveness of a male's sperm is not in sync with the countermeasures of his mate, some overzealous sperm could go rogue, causing a significant loss in fitness (Figure 4B). Thus, selection on males could favor highly migratory sperm that outcompete those from other males, even if they occasionally lower female fertility. In addition, selection in females should favor protective countermeasures that restore normal fertility but may decrease male fitness. This sexually antagonistic coevolution is expected to cause rapid changes in both sexes, which are revealed when animals from different species are used in experimental crosses.
(A) In a benign interaction between the sexes, male sperm compete for position in the spermatheca, and a combination of female signals and control of the distal spermathecal valve prevent them from entering the ovary. (B) Because of sperm competition, some males develop more competitive sperm that cannot be excluded from the ovary and thus become invasive; resulting fitness costs will favor additional female countermeasures through sexually antagonistic coevolution.
These studies show not only that nematodes undergo sperm competition but also that additional postcopulatory interactions are significant and appear to include sexually antagonistic coevolution. Given the broad range of interactions between sperm, oocytes, and chemical and physical cues inside the female reproductive tract (Figure 3) (reviewed in ), nematodes in the genus Caenorhabditis could provide valuable models for investigating postcopulatory sexual selection and sexual conflicts in the laboratory.
New isolates of different Caenorhabditis species are being established at a considerable rate (e.g., ), which should also permit study of postcopulatory mechanisms in a variety of wild populations. The detection of rogue sperm in some intraspecies crosses might be explained not only by the greater aggressiveness of sperm from dioecious species but also by the high levels of genetic diversity in these species , which should make it easier to detect mismatches between male persistence and female countermeasures. Thus, it would be fascinating to analyze the role that genetic variability in dioecious species plays in the range of sperm phenotypes and interactions and to see if the low levels of diversity in androdioecious species limit this range in the wild. If genetic diversity is important, it might even be possible to find specific strains of androdioecious species that produce ectopic sperm in crosses. Finally, we might be able to learn if some sperm traits and female countermeasures are restricted to isolated populations, which genes underlie these traits, and how fast these types of coevolutionary changes are occurring, at morphological, biochemical, and molecular levels. The combination of experimental and field approaches available to address these questions promises an exciting future.
- 1. Darwin C (1859) On the Origin of Species by Means of Natural Selection, or the Preservation of Favored Races in the Struggle for Life. London: John Murray.
- 2. Darwin C (1871) The Descent of Man and Selection in Relation to Sex. London: John Murray.
- 3. Andersson MB (1994) Sexual selection. Princeton (New Jersey): Princeton University Press.
- 4. Parker GA (1970) Sperm competition and its evolutionary consequences in the insects. Biol Rev 45: 525–567.
- 5. Parker GA (1998) Sperm competition and the evolution of ejaculates: towards a theory base. In: Birkhead TR, Møller AP, editors. Sperm competition and sexual selection. London: Academic Press. pp. 3–54.
- 6. Pizzari T, Parker GA (2009) Sperm competition and sperm phenotype. In: Birkhead TR, Hosken DJ, Pitnick S, editors. Sperm biology: an evolutionary perspective. London: Academic Press. pp. 207–245.
- 7. Eberhard WG (2009) Postcopulatory sexual selection: Darwin's omission and its consequences. Proc Natl Acad Sci U S A 106 Suppl 1: 10025–10032.
- 8. Pitnick S, Wolfner MF, Suarez SS (2009) Ejaculate-female and sperm-female interactions. In: Birkhead TR, Hosken DJ, Pitnick S, editors. Sperm biology: an evolutionary perspective. London: Academic Press. pp. 247–304.
- 9. Schärer L, Littlewood DT, Waeschenbach A, Yoshida W, Vizoso DB (2011) Mating behavior and the evolution of sperm design. Proc Natl Acad Sci U S A 108: 1490–1495.
- 10. Lange R, Reinhardt K, Michiels NK, Anthes N (2013) Functions, diversity, and evolution of traumatic mating. Biol Rev Camb Philos Soc 88: 585–601.
- 11. Howard DJ, Palumbi SR, Birge LM, Manier MK (2009) Sperm and speciation. In: Birkhead TR, Hosken DJ, Pitnick S, editors. Sperm biology: an evolutionary perspective. London: Academic Press. pp. 367–403.
- 12. Brenner S (1974) The genetics of Caenorhabditis elegans. Genetics 77: 71–94.
- 13. Ellis RE, Lin S-Y (2014) The evolutionary origins and consequences of self-fertility in nematodes. F1000Prime Rep In press.
- 14. Kiontke KC, Félix MA, Ailion M, Rockman MV, Braendle C, et al. (2011) A phylogeny and molecular barcodes for Caenorhabditis, with numerous new species from rotting fruits. BMC Evol Biol 11: 339.
- 15. Nuez I, Félix MA (2012) Evolution of Susceptibility to Ingested Double-Stranded RNAs in Caenorhabditis Nematodes. PLoS ONE 7: e29811.
- 16. Wei Q, Shen Y, Chen X, Shifman Y, Ellis RE (2014) Rapid creation of forward-genetics tools for C. briggsae using TALENs: lessons for nonmodel organisms. Mol Biol Evol 31: 468–473.
- 17. Barriere A, Félix MA (2005) Natural variation and population genetics of Caenorhabditis elegans. In: The C. elegans Research Community, editor. WormBook. Available: http://www.wormbook.org/chapters/www_naturalvariationgenetics/naturalvariationgenetics.pdf. Accessed 23 June 2014. doi/10.1895/wormbook.1.43.1
- 18. Sivasundar A, Hey J (2005) Sampling from natural populations with RNAi reveals high outcrossing and population structure in Caenorhabditis elegans. Curr Biol 15: 1598–1602.
- 19. Cho S, Jin SW, Cohen A, Ellis RE (2004) A phylogeny of Caenorhabditis reveals frequent loss of introns during nematode evolution. Genome Res 14: 1207–1220.
- 20. Kiontke K, Gavin NP, Raynes Y, Roehrig C, Piano F, et al. (2004) Caenorhabditis phylogeny predicts convergence of hermaphroditism and extensive intron loss. Proc Natl Acad Sci U S A 101: 9003–9008.
- 21. LaMunyon CW, Ward S (1995) Sperm precedence in a hermaphroditic nematode (Caenorhabditis elegans) is due to competitive superiority of male sperm. Experientia 51: 817–823.
- 22. LaMunyon CW, Ward S (1999) Evolution of sperm size in nematodes: sperm competition favours larger sperm. Proc Biol Sci 266: 263–267.
- 23. Baldi C, Viviano J, Ellis RE (2011) A bias caused by ectopic development produces sexually dimorphic sperm in nematodes. Curr Biol 21: 1416–1420.
- 24. LaMunyon CW, Ward S (1998) Larger sperm outcompete smaller sperm in the nematode Caenorhabditis elegans. Proc Biol Sci 265: 1997–2002.
- 25. LaMunyon CW, Ward S (2002) Evolution of larger sperm in response to experimentally increased sperm competition in Caenorhabditis elegans. Proc Biol Sci 269: 1125–1128.
- 26. Kubagawa HM, Watts JL, Corrigan C, Edmonds JW, Sztul E, et al. (2006) Oocyte signals derived from polyunsaturated fatty acids control sperm recruitment in vivo. Nat Cell Biol 8: 1143–1148.
- 27. Hoang HD, Prasain JK, Dorand D, Miller MA (2013) A heterogeneous mixture of F-series prostaglandins promotes sperm guidance in the Caenorhabditis elegans reproductive tract. PLoS Genet 9: e1003271.
- 28. Miller MA, Nguyen VQ, Lee MH, Kosinski M, Schedl T, et al. (2001) A sperm cytoskeletal protein that signals oocyte meiotic maturation and ovulation. Science 291: 2144–2147.
- 29. Kosinski M, McDonald K, Schwartz J, Yamamoto I, Greenstein D (2005) C. elegans sperm bud vesicles to deliver a meiotic maturation signal to distant oocytes. Development 132: 3357–3369.
- 30. Hill KL, L'Hernault SW (2001) Analyses of reproductive interactions that occur after heterospecific matings within the genus Caenorhabditis. Dev Biol 232: 105–114.
- 31. Ting JJ, Woodruff GC, Leung G, Shin N-R, Cutter AD, et al. (2014) Intense sperm-mediated sexual conflict promotes reproductive isolation in Caenorhabditis Nematodes. PLoS Biol 7: e1001915.
- 32. Baird SE, Sutherlin ME, Emmons SW (1992) Reproductive isolation in Rhabditidae (Nematoda: Secernentea); mechanisms that isolate six species of three genera. Evolution 46: 585–594.
- 33. Baird SE, Yen WC (2000) Reproductive isolation in Caenorhabditis: terminal phenotypes of hybrid embryos. Evol Dev 2: 9–15.
- 34. Baird SE (2002) Haldane's rule by sexual transformation in Caenorhabditis. Genetics 161: 1349–1353.
- 35. Woodruff GC, Eke O, Baird SE, Félix MA, Haag ES (2010) Insights into species divergence and the evolution of hermaphroditism from fertile interspecies hybrids of Caenorhabditis nematodes. Genetics 186: 997–1012.
- 36. Ellis RE, Stanfield GM (2014) The regulation of spermatogenesis and sperm function in nematodes. Semin Cell Dev Biol 29C: 17–30.
- 37. Dey A, Chan CK, Thomas CG, Cutter AD (2013) Molecular hyperdiversity defines populations of the nematode Caenorhabditis brenneri. Proc Natl Acad Sci U S A 110: 11056–11060.
- 38. Félix MA, Braendle C, Cutter AD (2014) A streamlined system for species diagnosis in Caenorhabditis (Nematoda: Rhabditidae) with name designations for 15 distinct biological species. PLoS ONE 9: e94723.