During gametogenesis and pre-implantation development, the mammalian epigenome is reprogrammed to establish pluripotency in the epiblast. Here we show that the histone 3 lysine 4 (H3K4) methyltransferase, MLL2, controls most of the promoter-specific chromatin modification, H3K4me3, during oogenesis and early development. Using conditional knockout mutagenesis and a hypomorph model, we show that Mll2 deficiency in oocytes results in anovulation and oocyte death, with increased transcription of p53, apoptotic factors, and Iap elements. MLL2 is required for (1) bulk H3K4me3 but not H3K4me1, indicating that MLL2 controls most promoters but monomethylation is regulated by a different H3K4 methyltransferase; (2) the global transcriptional silencing that preceeds resumption of meiosis but not for the concomitant nuclear reorganization into the surrounded nucleolus (SN) chromatin configuration; (3) oocyte survival; and (4) normal zygotic genome activation. These results reveal that MLL2 is autonomously required in oocytes for fertility and imply that MLL2 contributes to the epigenetic reprogramming that takes place before fertilization. We propose that once this task has been accomplished, MLL2 is not required until gastrulation and that other methyltransferases are responsible for bulk H3K4me3, thereby revealing an unexpected epigenetic control switch amongst the H3K4 methyltransferases during development.
It is well established that gametes and early mammalian embryos undergo extensive epigenetic changes, which are changes in phenotype or gene expression that do not entail changes in DNA sequence. However, the machinery responsible for epigenetic modification in these situations is poorly understood. In mice, we conditionally deleted the histone 3 lysine 4 (H3K4) methyltransferase Mll2, an enzyme that alters DNA structure and packaging, either in gametes or in somatic cells of the ovary and also produced a mouse hypomorph expressing low levels of MLL2. We show that MLL2 is required in oocytes during gametogenesis and is also needed as a maternally derived factor during early development. Oocytes deficient in Mll2 display decreased methylation of H3K4 (H3K4me3) and show abnormal maturation and gene expression, in particular of pro-apoptotic factors. In addition, we demonstrate that embryonic genome activation is compromised in the absence of Mll2. Together our results identify MLL2 as one of the key players in the epigenetic reprogramming required for female fertility in the mouse.
Citation: Andreu-Vieyra CV, Chen R, Agno JE, Glaser S, Anastassiadis K, Stewart AF, et al. (2010) MLL2 Is Required in Oocytes for Bulk Histone 3 Lysine 4 Trimethylation and Transcriptional Silencing. PLoS Biol 8(8): e1000453. doi:10.1371/journal.pbio.1000453
Academic Editor: Wolf Reik, The Babraham Institute, United Kingdom
Received: December 22, 2009; Accepted: July 2, 2010; Published: August 17, 2010
Copyright: © 2010 Andreu-Vieyra et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: This work was supported in part by National Institutes of Health grant HD33438 (to MMM), a Lalor Foundation fellowship (to CVA-V), the EU 6th Framework Integrated Program, HEROIC (LSHG-CT-2005-018883; to AFS), and the Deutches Forschung Gemeinschaft Schwerpunktprogram SPP1356, Pluripotency and Reprogramming (to AFS). The funding agencies had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
Abbreviations: Amhr2, anti-Mullerian hormone receptor 2; Bax, Bcl2-associated X protein; Casp6, caspase 6; Cdkn1a, cyclin-dependent kinase inhibitor 1A; cKO, conditional knockout; EZH2, Enhancer of Zeste 2; Gdf9, growth differentiation factor 9; H3K4, histone 3 lysine 4; Iap, intracisternal A particle; MLL, mixed lineage leukemia; NSN, non-surrounding nucleolus; PMSG, pregnant mare serum gonadotropin; Setd7, SET domain lysine methyltransferase 7; SN, surrounded nucleolus; WT, wild type; YFP, yellow fluorescence protein; ZGA, zygotic genome activation; Zp3, zona pellucida 3
Mammalian epigenomes are fundamentally reprogrammed during gametogenesis and pre-implantation development to establish the ground state of pluripotency in the epiblast cells of the blastocyst –. Despite the importance of this epigenetic reprogramming, how such changes are achieved is not well understood. Epigenetic reprogramming of the maternal genome occurs during oogenesis. Oocytes develop synchronously from birth until puberty, during which time they are arrested in meiotic prophase I, increase in size, and are transcriptionally active until the peri-ovulatory stage, when they undergo global transcriptional silencing ,. Transcription of the oocyte genome serves to establish the reservoirs of maternal components that are required for the first stages of embryonic development . Global transcriptional silencing in oocytes is thought to be required for the efficient resumption and completion of meiosis  and occurs parallel to large-scale chromatin condensation and rearrangement around the nucleolus to establish a chromatin configuration termed SN (surrounded nucleolus) ,,. Previous studies showed that global transcriptional silencing can occur without the establishment of the SN state ,. Further studies indicated that both the acquisition of the SN configuration and transcriptional silencing are pre-requisites to achieve full embryonic developmental potential ,.
Chromatin and epigenetic changes during oocyte development include histone variant exchange, alterations of DNA methylation, and global shifts in histone post-translational modifications. For instance, maternal-specific genomic imprints are established on a locus-by-locus basis –, the linker histone 1 variant H1FOO is incorporated into chromatin ,, and the global levels of 5-methyl-cytosine (CpG DNA methylation) and histone 4 acetylation at lysine 5 and 12 (H4K5 and H4K12) increase . In addition, the levels of di- and tri-methylation of histone 3 at lysines 4 and 9 (H3K4me2/3 and H3K9me2/3), which are associated with active and repressed gene expression, respectively ,, both increase and peak at the peri-ovulatory stage .
After fertilization and the second meiotic division, and between the late 1-cell and 2-cell embryo stages in the mouse, the zygotic genome is activated (ZGA) –. The maternal and paternal genomes of mouse 1-cell embryos display asymmetric distributions of histone H3 variants as well as di- and tri-methylation of H3K9, H3K4, H3K27, and H3K20, whereas H3K4 and H3K20 monomethylation levels are similar between both pronuclei –. The asymmetry in H3K9 methylation has been proposed to protect the female pronucleus from global CpG demethylation, which takes place in the paternal pronucleus soon after fertilization ,. In contrast, the significance of the asymmetry of other histone modifications remains unclear.
Histone 3 tail modifications are central to epigenetic regulation, and given the extent of epigenetic reprogramming that takes place during gametogenesis and early development, it is likely that mechanisms regulating H3 methylation play significant roles. However, few functional relationships have been identified. For instance, loss of the H3K27 methyltransferase Enhancer of Zeste 2 (EZH2) causes early post-implantation embryonic lethality . The absence of maternal EZH2 results in neonatal growth retardation despite the rescue of embryonic lethality by transcription from the paternal allele . This suggests a role for EZH2 in oogenesis and/or very early development. Furthermore, Embryonic Ectoderm Development (Eed), which is a subunit of the EZH2 H3K27 methyltransferase complex PRC2, is involved in genomic imprinting , indicating that EZH2 and H3K27 methylation play, at least, one role in epigenetic reprogramming. In most other cases studied so far, the embryonic lethality caused by loss of an H3 methyltransferase activity has precluded the analysis of how they function in gametogenesis and epigenetic reprogramming.
Tri-methylation of H3K4 marks promoters in all eukaryotes, whereas di-methylation and mono-methylation are associated with transcribed regions and active chromatin ,. In mammals, H3K4 methylation is mediated by at least six enzymes related to fly Trithorax an d the original H3K4 methyltransferase, yeast Set1 . These include MLL1 (Mixed lineage leukemia 1) and MLL2 ,. Different H3K4 methyltransferases display some overlapping functions; however, they also have unique functional aspects, since MLL1 and MLL2 are both required for development . MLL2 is also required for spermatogenesis, as revealed by ubiquitous conditional mutagenesis in adult males .
Here we report that MLL2 is required for female fertility. Using conditional knockout mutagenesis of Mll2 in oocytes or ovarian granulosa cells, we found that MLL2 is required for several processes during oocyte postnatal development but is dispensable for granulosa cell function. Surprisingly, we found that MLL2 is essential for bulk H3K4 methylation in peri-ovulatory oocytes, indicating that MLL2 function cannot be compensated by other H3K4 methyltransferases in this cell type. We found that mice with reduced expression of Mll2 (hypomorph allele, Mll2tm2) showed decreased fertility and a similar, albeit milder phenotype than that of oocyte-specific cKO mice. Mll2tm2/tm2 females demonstrate pre-implantation embryonic lethality likely due to impaired ZGA. Our results favor the hypothesis that MLL2 is required during post-natal oogenesis and early preimplantation development, thereby identifying MLL2 as one of the key players in the epigenetic reprogramming required for female fertility in the mouse.
Oocyte and Granulosa Cell-Specific Conditional Knockouts
The epigenome in the female pronucleus of zygotes is established in oocytes , under both autonomous and ovarian control . We first evaluated MLL2 expression in ovarian cells and during embryogenesis by PCR analysis. The results show that both ovarian granulosa cells and oocytes from wild type (WT) females express Mll2 (Figure 1A). QPCR analysis of meiotically incompetent (immature) and peri-ovulatory oocytes revealed a 5-fold increase in Mll2 expression as oocytes approach ovulation, suggesting a potential role for MLL2 during fertilization and/or early embryogenesis (Figure 1B; n = 3; Student's t test; p<0.05). Mll2 mRNA was present in 1-cell embryos, likely as a maternal product, and it was expressed from the 2-cell stage to the blastocyst stage as determined by QPCR analysis (Figure 1C).
(A) RT-PCR analysis of Mll2 showed expression in ovarian granulosa cells (GC) and peri-ovulatory (GV) oocytes (Oo) as determined. (B) Real time PCR (QPCR) analysis of meiotically incompetent (immature) oocytes from 12-d-old females and mature peri-ovulatory (GV, germinal vesicle) oocytes showed a significant increase in Mll2 mRNA levels towards the peri-ovulatory stage (Student's t test, * p<0.05; three pools of oocytes were used in the analysis; n = 3). Results are shown relative to Mll2 levels in GV oocytes (RQ). Gapdh was used as endogenous control. (C) QPCR analysis of preimplantation embryos revealed that Mll2 is present in 1-cell embryos, likely as a maternal product, and also in subsequent embryonic stages up to the blastocyst (Bl) stage. Gapdh was used as endogenous control. Results are shown as arbitrary units (delta CT: CT values corrected by the endogenous control). Abbreviations: GV, germinal vesicle oocytes; 1-C, 1 cell embryos; 2-C, 2 cell embryos; 4-C, 4 cell embryos; M, morula; Bl, blatocyst. (D) cKO mice were generated by tissue-specific deletion of the Mll2 floxed (“F”) allele in the Mll2 null allele background. The “F” allele was generated after FLP recombination to remove the stop cassette. Cre recombination eliminates the second exon, thereby causing a frame-shift (“FC” allele). (E) Breeding scheme used to generate Mll2Gdf9 cKO mice. (F) Mll2Gdf9 cKO oocytes show a significant reduction in Mll2 mRNA levels (Student's t test, * p<0.05). Results are shown as means ± S.E. relative to WT (RQ) (three oocyte pools were used; n = 3), and Gapdh was used as endogenous control. (G) Mll2Gdf9 cKO oocytes show negligible levels of MLL2 protein as assessed by Western blot analysis; tubulin was used as loading control. (H) Mll2Gdf9 cKO females were infertile; the cumulative number of pups over a 6-mo period is shown (n = 10).
We used a floxed conditional allele (Mll2F) to dissect MLL2 function (Figure 1D). Previous studies with this allele and a tamoxifen-inducible Cre mouse line showed that near-ubiquitous loss of Mll2 in adult males led to sterility . Here we show that near-ubiquitous loss of Mll2 in adult females also leads to sterility (Figure S1), further suggesting roles for Mll2 in gametogenesis. Because Mll2 is expressed in both granulosa cells and oocytes, we used cell-type specific conditional mutagenesis of the conditional Mll2 allele. Conditional knockout (cKO) mice were generated using Cre recombinase driven by the growth differentiation factor 9 (Gdf9) and zona pellucida (Zp3) promoters (Figure 1E), which are expressed in oocytes ,) or by the anti-Mullerian hormone receptor 2 (Amhr2) promoter, which is expressed primarily in ovarian granulosa cells in adult females . It is noteworthy that expression of Gdf9-Cre in the oocyte occurs in all follicular stages from postnatal day 3, whereas expression of Zp3-Cre begins at postnatal day 5 from the primary follicular stage onwards .
Mll2 Deletion Mediated by Gdf9-Cre Results in Female Sterility and Premature Ovarian Follicle Loss
The effect of Cre recombination on Mll2 expression in oocytes was examined by QPCR analysis. Oocytes from Mll2FC/−,Gdf9-Cre+ females (herein called Mll2Gdf9 cKO) displayed an 80% decrease in Mll2 mRNA expression compared to WT controls (Figure 1F). Cre recombination deletes exon 2 causing a frame-shift and thus no functional protein is produced. Concomitantly, mRNA levels fall presumably due to nonsense mediated decay . This was reflected by the presence of negligible levels of protein in isolated peri-ovulatory oocytes from Mll2Gdf9 cKO females (Figure 1F). Next, by repeated breeding to males of known fertility, we found that Mll2Gdf9 cKO females were sterile (Figure 1G). To begin to understand the basis of the infertility displayed by Mll2Gdf9 cKO mice, we analyzed serum hormone levels. Eight-week-old Mll2Gdf9 cKO females displayed increased serum gonadotropin hormones (FSH: WT, 4.24±0.43 ng/ml; Mll2Gdf9 cKO, 23.81±1.99 ng/ml; and LH: WT, 0.14±0.03 ng/ml; Mll2Gdf9 cKO, 0.87±0.17 ng/ml; n = 5; p<0.05) and decreased serum estradiol (WT, 21.66±3.2 ng/ml; Mll2Gdf9 cKO, 12.24±1.0 ng/ml; n = 5; p = 0.06), suggesting abnormal folliculogenesis.
To investigate further this potential defect, histological sections of ovaries from Mll2Gdf9 cKO females of various ages were evaluated and follicle counts were performed. Based on morphology , follicles were grouped into three categories: dormant (primordial), growing (primary, secondary, preantral, and antral), or dying (atretic and zona pellucida remnants). Two-week-old Mll2Gdf9 cKO ovaries displayed a higher number of primary growing follicles (Figure 2A and 2B, upper panels), suggesting increased follicular recruitment. Three-week-old Mll2Gdf9 cKO ovaries showed a 50% decrease in dormant follicles and an increase in growing preantral follicles (Figure 2A and 2B, middle panels), whereas 8-wk-old Mll2Gdf9 cKO ovaries displayed an 80% reduction in dormant follicles (Figure 2A and 2B, lower panels), demonstrating increased follicle loss. Further, the number of atretic follicles was significantly increased in Mll2Gdf9 cKO females from 3 wk of age onwards (Figure 2D; n = 5, ANOVA test, p<0.05). Older (36-wk-old) Mll2Gdf9 cKO ovaries showed few follicles (Figure 2C). Together, the results suggest that increased early follicle recruitment and oocyte loss contributes to infertility of Mll2Gdf9 females.
(A) PAS-stained ovaries from 2-, 3-, and 8-wk-old mice; the number of follicles was greatly reduced in ovaries from 8-wk-old Mll2Gdf9 cKO mice. (B) Follicle counts from 2-, 3-, and 8-wk-old mice. At 2 wk, Mll2Gdf9 cKO ovaries showed significantly increased (ANOVA test, * p<0.05) primary follicles (Pr), whereas 3-wk-old Mll2Gdf9 cKO ovaries showed significantly reduced primordial follicles (Prl) and increased preantral follicles (PA) (ANOVA test, * p<0.05). Mll2Gdf9 cKO ovaries from 8-wk-old mice showed further reduction in Prl follicles (ANOVA test, * p<0.05). Means of follicle counts corrected by surface area ± S.E. are shown (ovarian sections from five individual females were used; n = 5). Abbreviations: Prl, primordial; Pr, primary; Sec, secondary; A, antral; CL, corpora lutea. (C) Mll2Gdf9 cKO ovaries from 36-wk-old mice stained with hematoxylin-eosin showed very few follicles (boxed area). (D) From 3 wk onwards, the number of atretic follicles was significantly higher in Mll2Gdf9 cKO ovaries, compared to controls. Means of follicle counts corrected by surface area ± S.E. are shown (ovarian sections from five individual females were used; n = 5). ANOVA test, * p<0.05.
Mll2Gdf9 cKO Females Fail to Ovulate
By stimulation of pre-pubertal mice with gonadotropin hormones to induce ovulation and resumption of meiosis, we found that Mll2Gdf9 cKO females were essentially anovulatory (Figure 3A; n = 4, Student's t test, p<0.05). Anovulation was confirmed by histologycal analysis of ovaries from hormonally stimulated females, which showed oocytes trapped in luteinizing structures (Figure 3B). To determine whether resumption of meiosis was also compromised, we cultured isolated peri-ovulatory oocytes and found that the majority of Mll2Gdf9 cKO oocytes progressed to meiosis II, whereas a small but significant fraction remained arrested in meiosis I (Figure 1C and 1D; ANOVA test; p<0.05).
(A) Ovulation rates were significantly reduced in 3-wk-old superovulated Mll2Gdf9 cKO females (Student's t test, ** p<0.01). Means ± S.E. are shown (five individual females were used; n = 5). (B) PAS-stained ovaries from superovulated 3-wk-old Mll2Gdf9 cKO mice showed trapped oocytes (yellow arrowheads), which correlated with decreased ovulation rates in this mouse line. (C) In vitro oocyte maturation studies; representative single plane confocal laser microscopy micrographs of peri-ovulatory oocytes cultured for 16 h to allow resumption of meiosis; arrows show lagging chromosomes. Magnification: 800×. Abbreviations: MI, meiosis I; MII, meiosis II; PB, polar body. (D) Percentages of meiosis I (MI), meiosis II (MII), or abnormal oocytes after 16 h in culture (ANOVA test, * p<0.05). Means ± S.E. of four independent experiments are shown; a total of 120 oocytes per genotype were analyzed.
Mll2Gdf9 cKO Oocytes Fail to Establish Transcriptional Repression and Show Abnormal Histone Tail Modifications
During folliculogenesis, oocytes acquire a number of epigenetic marks ,, undergo large-scale chromatin rearrangement from a non-surrounding (NSN) to a surrounded (SN) nucleolus configuration, and establish a state of global transcriptional repression . Confocal microscopy analysis showed that the percentage of peri-ovulatory oocytes from WT and Mll2Gdf9 cKO females that acquired the expected SN configuration  was comparable (Figure 4A). However, using a run-on assay based on Br-UTP incorporation into nascent RNA, we found that, unlike controls, 80% of the Mll2Gdf9 cKO oocytes were still transcriptionally active at the peri-ovulatory stage (Figure 4B, upper and lower panels; Student's t test, p<0.05). Furthermore, treatment with α-amanitin reduced Br-UTP incorporation , indicating that transcription was RNA Pol II-dependent (Figure 4B). Hence, our results show that MLL2 is required for oocyte transcriptional silencing and confirm that the establishment of the SN configuration does not depend on the acquisition of global transcriptional repression .
(A) Confocal laser microscopy analysis of peri-ovulatory oocytes stained with propidium iodide to visualize DNA (upper panel); oocytes were scored as displaying a surrounded nucleolus (SN) or a non-surrounded nucleolus (NSN) configuration (lower panel). Arrows show the nucleolus. No significant differences were observed between controls and Mll2Gdf9 cKO oocytes (ANOVA test, * p = 0.08). Means ± S.E. are shown; a total of 170 oocytes from five individual females were analyzed in three independent experiments. Magnification: 800×. (B) Run-on and confocal microscopy analyses of Br-UTP labeled nascent RNA in peri-ovulatory oocytes. Upper panel: Representative single plane confocal micrographs are shown as merge and split channels (Br-UTP, green staining; and DNA: propidium iodide, red staining). Lower panel: oocytes were scored as positive or negative for Br-UTP staining; note that Mll2Gdf9 cKO oocytes were transcriptionally active (ANOVA test, * p<0.05). Transcription was RNA Pol II dependent as it was abrogated by α-amanitin (αA) (magnification: 800×). Means ± S.E. are shown; a total of 90 oocytes from four individual females were evaluated in three independent experiments.
Histone tail lysines can be mono-(me1), di-(me2), or tri-(me3) methylated . In particular, H3K4me3 is found at the promoters of genes that are transcriptionally active or poised for activity –. Because MLL2 is a H3K4 histone methyltransferase, we next analyzed the methylation status of H3K4. Confocal microscopy analysis of Mll2Gdf9 cKO oocytes showed an apparent increase in inmunostaining of H3K4me1 (Figure 5A) and a decrease in immunostaining of H3K4me2 and H3K4me3 (Figure 5B and 5C), whereas control oocytes displayed the expected distribution of methylation marks (Figure 5A–C) . Western blot analysis of chromatin fractions followed by chemoluminescence quantification confirmed that Mll2Gdf9 cKO oocytes have significantly lower levels of H3K4me2 and H3K4me3 and significantly higher levels of H3K4me1 (Figure 5D and 5E; Student's t test; p<0.05). Interestingly, confocal microscopy and Western blot analyses showed that acetylated H4K12, a modification associated with active chromatin , was also significantly increased in Mll2Gdf9 cKO oocytes (Figure 5F–5H; Student's t test; p<0.05). In contrast, Mll2Gdf9 cKO oocytes displayed global levels of repressive marks such as H3K9me3, H3K27me3, and H4K20me1, or those of acetylated H3 that were comparable to those of controls (Figure 5I).
(A–C) Confocal microscopy micrographs showing H3K4 methylation levels in peri-ovulatory oocytes. (A) An increase in H3K4me1 is apparent in Mll2Gdf9 cKO oocytes (lower panel) compared to control (upper panel). In contrast, a decrease in H3K4me2 (B) and H3K4me3 (C) levels was observed in Mll2Gdf9 cKO oocytes (B and C, lower panels) compared to controls (B and C, upper panels). Magnification: 800×. Representative single plane micrographs are shown as merge and split channels of histone tail modifications (green) and DNA (red). (D–E) Western blot analysis of H3K4 methylation in chromatin fractions from peri-ovulatory oocytes. (D) Representative micrographs of Western blots showing H3K4me1, H3K4me2, and H3K4me3 levels. (E) Chemoluminescence quantification revealed a significant increase in global H3K4me1 and a significant decrease in global H3K4me2 and H3K4me3 levels in Mll2Gdf9 cKO oocytes; Student's t test, * p<0.05; ** p<0.01. Means ± S.E are shown. Three pools of 100 oocytes each from 3–4 females per genotype were used in three independent experiments; samples were normalized against total histones. (F) Confocal microscopy micrographs showing H4K12 acetylation levels in peri-ovulatory oocytes. An increase in H4K12 acetylation is apparent in Mll2Gdf9 cKO oocytes (lower panel) compared to controls (upper panel). Magnification: 800×. Representative single plane micrographs are shown as merge and split channels of histone tail modifications (green) and DNA (red). (G–H) Western blot analysis of H4K12 acetylation (H3K12(ac)) in chromatin fractions from peri-ovulatory oocytes. (G) Representative micrographs of Western blots showing H4K12 acetylation levels. (H) Chemoluminescence quantification revealed a significant increase in global H4K12 acetylation in Mll2Gdf9 cKO oocytes; Student's t test, * p<0.05; Means ± S.E are shown. Three pools of 100 oocytes each from 3–4 females per genotype were used in three independent experiments; samples were normalized against total histones. (I) Western blot analysis of other histone tail modifications in chromatin fractions from peri-ovulatory oocytes. Representative micrographs of Western blots showing H3K9me2, H3K27me3, H4K20me1, and H3(ac); total histones were used as internal loading controls. No changes were observed in the levels of any of the histone tail modifications analyzed. Three pools of 100 oocytes each from 3–4 females per genotype were used in three independent experiments.
Together, the results suggest that MLL2 is responsible for the majority of maternal H3K4me3 and that other histone methyltransferases cannot compensate MLL2 function in this cell type. Loss of Mll2 does not affect the establishment/maintenance of other histone post-translational modifications. Importantly, our results show that Mll2 deficiency leads to a decrease in H3K3me3 but not H3K4me1, suggesting that a histone methyltransferase other than MLL2 is responsible for this methylation mark. The results also indicate that transcriptional repression in peri-ovulatory oocytes is defective in the absence of MLL2. It is unclear whether this phenomenon is a consequence of altered H3K4 methylation, defects in granulosa cell-oocyte communication, or other factors. However, the increased H4K12 acetylation likely reflects the lack of transcriptional silencing observed in Mll2Gdf9 cKO oocytes.
Abnormal Expression Pro-Apoptotic Genes and Iap Elements in Mll2Gdf9 cKO Oocytes
Elevated transcription in Mll2Gdf9 cKO peri-ovulatory oocytes prompted further investigation of potential gene expression changes. A survey of selected genes involved in oocyte function – and early embryogenesis – by QPCR analysis of peri-ovulatory oocytes revealed no changes in the majority of genes investigated (Table 1). An exception was the B-type cyclin E3 ubiquitin ligase cyclin B1 interacting protein 1 (Ccnb1ip, also known as Hei10), which is important for both meiosis and embryogenesis . Ccnb1ip/Hei10 expression was significantly decreased in Mll2Gdf9 cKO oocytes (p<0.05; Table 1). Interestingly, expression of the histone 4 acetylase Kat5/Tip60 (Lysine acetyltransferase 5), which has been previously reported to be present in oocytes, was significantly increased in Mll2Gdf9 cKO oocytes (Table 1, p<0.05)  and may therefore contribute to the observed increase in H4K12 acetylation.
QPCR analysis also showed that Mll2Gdf9 cKO oocytes overexpressed several apoptosis-associated genes (Table 1), including p53 (transformation related protein 53; TRP53) and Setd7 (SET domain lysine methyltransferase 7, also know as Set7/9) (Table 1 and Figure S2A), which methylates p53 and prevents its degradation . P53 has been implicated in prenatal oocyte apoptosis, and peri-ovulatory oocytes express low p53 levels . Consequently, it is possible that up-regulated SETD7 stabilizes p53 in Mll2Gdf9cKO oocytes leading to increased oocyte death. Supporting this hypothesis, we found high levels of p53 protein (Figure S2B) and upregulation of several pro-apoptotic p53 targets, including Bax (Bcl2-associated X protein), Cdkn1a (cyclin-dependent kinase inhibitor 1A; P21), Fos (FBJ murine osteosarcoma viral oncogene homolog) ,, and Casp6 (caspase 6) , in Mll2Gdf9 cKO oocytes (Figure S2C and Table 1). Since follicular atresia is thought to occur via apoptosis, the increase in expression of pro-apoptotic factors is in line with the increase in the number of atretic follicles observed in Mll2Gdf9 cKO ovaries.
Finally, because of the widespread lack of transcriptional repression in Mll2Gdf9 cKO oocytes, we looked at the expression of retrotransposon interspersed repeats by QPCR analysis. We found that intracisternal A particle (Iap) elements were abnormally transcribed in Mll2Gdf9 cKO peri-ovulatory oocytes, whereas LINE-1 and SINE-1 elements were not (Figure S3A). Increased Iap expression in Mll2Gdf9 cKO oocytes correlated with hypomethylation of Iap DNA loci, as evaluated by methylation specific primers (Figure S3B) and bisulphite sequencing (Figure S3C). However, Mll2Gdf9 cKO oocytes showed a strong signal for 5-methyl-cytosine staining, indicating that loss of CpG methylation was not a widespread phenomenon (Figure S3D). Because retrotransposons drive the expression of multiple host genes in female gametes , Iap up-regulation could contribute to loss of Mll2Gdf9 cKO oocytes. Absence of Uhrf1 (ubiquitin-like, containing PHD and RING fingers 1 also known Np95) in cell lines and mouse embryos has been associated with increased transcription of interspersed repeats due to DNA hypomethylation . Consequently, we examined Uhrf1 expression levels and found a 2-fold decrease (Table 1), suggesting a role in Iap deregulation. Decreased Uhrf1 in Mll2Gdf9 cKO oocytes could also contribute to Cdkn1a deregulation . Together, the results suggest that stabilization of p53 and abnormal expression of its downstream targets as well as deregulated expression of retrotransposable elements may trigger apoptosis in Mll2Gdf9 cKO oocytes, leading to oocyte death and, ultimately, premature follicle loss and sterility.
Deletion of Mll2 Using Zp3-Cre
To further substantiate the phenotype observed in the Mll2Gdf9 cKO mouse model, we employed conditional mutagenesis using the Zp3-Cre allele (Figure 6A). Peri-ovulatory oocytes from Mll2FC/−, Zp3Cre+ females (herein called Mll2Zp3 cKO) show a significant decrease in Mll2 expression as evaluated by QPCR analysis (Figure 6B; Student's t test; p<0.05; n = 3). Similar to Mll2Gdf9 cKO mice, Mll2Zp3 cKO females showed a significant increase in FSH levels (Figure 6C) and were infertile (Figure 6D). However, Mll2Zp3 cKO females showed a milder rate of follicule loss than that of Mll2Gdf9 cKO mice, as evidenced by the lack of significant changes in the number of atretic follicles at 3 wk of age (Figure 6E and 6F) and the presenc of follicles at 36 wk of age (Figure 6E). In contrast to Mll2Gdf9 cKO females, which were anovulatory, Mll2Zp3cKO mice displayed an ovulation rate of 20%–25% compared to controls (Figure 6G). Consistent with decreased ovulation rates, Mll2Zp3 cKO ovaries showed a number of oocytes trapped in luteinizing structures (Figure 6H). Interestingly, oocytes ovulated by Mll2Zp3 cKO females were capable of fertilization in vivo, and the majority of embryos generated from matings of Mll2Zp3 cKO females and WT males became developmentally arrested between the 1-cell and 2-cell stages (Figure 6I). Confocal microscopy analysis of Mll2Zp3 cKO oocytes showed an increase in the levels of H3K4me1 (Figure 7A) and a reduction in the levels of H3K4me3 (Figure 7B) comparable to that observed in Mll2Gdf9 cKO oocytes (Figure 5A–C). Western blot analysis confirmed the decrease in bulk H3K4me3 in Mll2Zp3cKO oocytes (Figure 7C). These results demonstrate that Mll2Zp3 cKO females display a similar but slightly milder phenotype than Mll2Gdf9 cKO females, consistent with the later onset of Cre expression in the Zp3-Cre line . Importantly, the results show that MLL2 is required not only during oogenesis but also for early embryogenesis.
(A) Breeding scheme used to generate Mll2Zp3cKO mice. (B) Zp3-cKO oocytes show a significant reduction in Mll2 mRNA levels (Student's t test, * p<0.05). Results are shown as means ± S.E. relative to WT (RQ) (three oocyte pools were used; n = 3) and Gapdh was used as endogenous control. (C) FSH levels were significantly higher in serum samples from 8-wk-old Mll2Zp3 cKO females compared to controls (Student's t test, * p<0.05; n = 10). (D) Fertility studies shown as cumulative number of pups over a 6-mo period. Mll2Zp3cKO females were infertile. (G) Ovulation rates were significantly reduced in Mll2Zp3cKO females compared to controls (WT) (Student's t test; * p<0.05; n = 5). (E) PAS-stained ovaries from 3- and 36-wk-old mice. Original magnification: 25× and 50×; note that different from Mll2Gdf9 cKO mice, Mll2Zp3 cKO ovaries still contain follicles at 36 wk of age. (F) Follicle counts in Mll2Zp3 cKO ovaries. Mll2Zp3 cKO ovaries had a significantly lower number of primordial follicles and increased numbers of secondary and multilayer preantral follicles 3 wk of age (left panel). By 8 wk of age (right panel), the number of primordial and multilayer preantral follicles is significantly reduced and the number of atretic follicles is increased in Mll2Zp3 cKO ovaries. (ANOVA test; * p<0.05; ovarian sections from five females were used in the analysis; n = 5). Abbreviations: Prl, primordial; Pr, primary; Sec, secondary; PA, preantral; A, antral; CL, corpora lutea; Atr, atretic. (I) Cultures of embryos from Mll2Zp3 cKO females crossed to WT males showed a developmental arrest between the 1-cell and the 4-cell stages. Results are presented as the % embryos (average) ± standard error from five independent experiments. ANOVA test; * p<0.05. A total of 95 embryos from six females per genotype were used in three independent experiments. Abbreviations: C, cell; Frag, fragmented; WT, control wild type.
(A–B) Confocal microscopy micrographs showing H3K4 methylation levels in peri-ovulatory oocytes. (A) An increase in H3K4me1 is apparent in Mll2Zp3 cKO oocytes (lower panel) compared to control (upper panel). In contrast, a decrease in H3K4me3 (B) levels was observed in Mll2Zp3 cKO oocytes (lower panel) compared to controls (upper panel). Magnification: 800×. Representative single plane micrographs are shown as merge and split channels of histone tail modifications (green) and DNA (red). (C) Representative micrographs of Western blots showing H3K4me3 levels in chromatin fractions from Mll2Zp3 cKO peri-ovulatory oocytes. A decrease in global H3K4me3 is apparent in Mll2Zp3c KO oocytes (Student's t test, * p<0.05). Three pools of 100 oocytes each from 3–4 females per genotype were used in three independent experiments. Total histones were used as internal loading controls.
Mll2 Deletion in Granulosa Cells Has Limited Impact on Female Fertility
To evaluate whether the loss of MLL2 in ovarian granulosa cells affects female fertility, we generated a mouse line carrying a granulosa cell deletion of Mll2 (Mll2FC/−, Amhr2-Cre, herein called Mll2Amhr2 cKO) (Figure 8A). In contrast to the oocyte cKOs (Mll2Gdf9 cKO and Mll2Zp3 cKO), the loss of Mll2 in granulosa cells had only a small impact on fertility. Amhr2-Cre action on Mll2F/F resulted in ∼75% reduction in granulosa cell Mll2 mRNA levels (Figure 8B) and an almost complete loss of protein in Mll2Amhr2 cKO granulosa cells as expected from the conditional allele design (Figure 8C). In young mice, Mll2 deficiency in granulosa cells had little impact on fertility (Figure 8D), ovulation or fertilization rates (Mll2Amhr2: 45.0±3.1; control: 37.0±2.5, n = 4), gonadotropin hormone levels (FSH: control, 6.33±1.39 ng/ml; Mll2Amhr2 cKO, 9.51±2.57 ng/ml; LH: control, 0.26±0.07 ng/ml; Mll2Amhr2 cKO, 0.17±0.08 ng/ml; n = 7), or ovarian histology (unpublished data). However, older animals showed a small reduction in fertility (Figure 8D), abnormal ovarian histology, and increased follicle loss (Figure 8E and 8F), as evidenced by a significant increase in the number of atretic follicles and a significant decrease in the number of growing follicles (Figure 8F; ANOVA test, p<0.05). Follicle loss correlated with increased FSH levels in Mll2Amhr2 cKO mice (control, 5.31±0.82 ng/ml; Mll2Amhr2 cKO, 10.83±0.83 ng/ml; n = 7). Analysis of H3K4 methylation levels by Western blot of Mll2Amhr2 cKO granulosa cell chromatin fractions showed no changes in the levels of H3K4me1 or H3K4me2 (Figure 8G and 8H). A 50% decrease in global H3K4me3 was observed in Mll2Amhr2 cKO granulosa cells (Figure 8G and 8H; Student's t test, p<0.05); however, no differences were found in the levels of H3K27me3 between WT and Mll2Amhr2 cKO granulosa cells (Figure 8I). Finally, QPCR analysis of other H3K4 methyltransferases showed a significant increase in the expression of Mll3 (WT: 1.46±0.07; Mll2Amhr2 cKO: 2.48±0.47; n = 3, p<0.05) but not Mll1 (WT: 1.08±0.08; Mll2Amhr2 cKO: 1.53±0.47; n = 3) in Mll2Amhr2 cKO granulosa cells. The results indicate that MLL2 is not essential for granulosa cell function and that an increase in Mll3 in Mll2Amhr2 cKO granulosa cells may, at least partially, compensate for Mll2 loss.
(A) Breeding scheme used to generate granulosa cell-specific cKO mice (Mll2Amhr2 cKO). (B) QPCR analysis of Mll2 levels in granulosa cells from wild type (WT), Mll2F/− (heterozygous control), and Mll2Amhr2 cKO females (granulosa cells from three individual females were used in the analysis; n = 3). Mll2Amhr2 cKO granulosa cells show a significant decrease in the levels of Mll2, whereas Mll2F/− granulosa cells show the expected 50% decrease in Mll2 levels (ANOVA test; * p<0.05). Gadpdh was used as internal control. (C) Western blot analysis of Mll2Amhr2 cKO granulosa cells showed decreased MLL2 protein levels. Tubulin was used as loading control. (D) Fertility studies shown as cumulative number of pups over a 6-mo period. Mll2Amhr2 cKO females displayed a small reduction in the number of pups (n = 10). (E) PAS-stained ovaries from 36-wk-old Mll2Amhr2 cKO mice showed PAS-positive zona pellucida remnants (oocyte remnants, arrowheads). Note that the number of follicles present at this age is higher than that observed in the oocyte-specific cKO mouse lines. (F) Follicle counts in 36-wk-old ovaries. Mll2Amhr2 cKO ovaries show a significant decrease in the number of quiescent amd growing follicles and a significantly higher number of atretic follicles (ovarian sections from five females were used in this study; n = 5). Abbreviations: Prl, primordial; Pr, primary; Sec, secondary; PA, preantral; A, antral; CL, corpora lutea. ANOVA test; * p<0.05. (G–H) Western blot analysis of H3K4 methylation in chromatin fractions from granulosa cells. (G) Representative micrographs of Western blots showing H3K4me1, H3K4me2, and H3K4me3 levels. (H) Chemoluminescence quantification revealed a significant decrease in global H3K4me3 levels in Mll2Amhr2 cKO granulosa cells (Student's t test, * p<0.05). Means ± S.E are shown. Granulosa cells from three individual females per genotype were used in three independent experiments; samples were normalized against total histones. (G) Representative Western blot analysis of the repressive mark H3K27me3 showed no global changes in Mll2Amhr2 cKO granulosa cells. Total histones were used as internal loading control.
Mll2tm2/tm2 Female Mice Show Reduced Fertility
We generated an YFP-Mll2 fusion protein by gene targeting in ES cells and name the allele targeted mutation 2 (Mll2tm2; Figure S4A). Homozygous mice carrying the Mll2tm2 allele (Mll2tm2/tm2) were viable (Figure S4B and S4C); however, they showed no detectable yellow fluorescent signal in any tissue (unpublished data). To determine whether YFP-Mll2 was expressed, we analyzed Mll2tm2/tm2 ovaries by real-time PCR and Western blot and found a significant decrease in both mRNA (Figure S4D) and protein (Figure S4E) levels, indicating that the mice carrying this allele were hypomorphs for MLL2. We next examined the fertility of Mll2tm2/tm2 females by mating them to males of known fertility. From the first month of mating, Mll2tm2/tm2 females showed a 50% reduction in the number of pups per litter compared to WT or Mll2tm2/+ controls, and showed impaired cumulative fertility with age (Figure S4F). Notably, the fertility of Mll2tm2/+ females was also cumulatively reduced (Figure S4F).
Embryos from Mll2tm2/tm2 Females Show Decreased In Vitro Developmental Potential
Ovaries from Mll2tm2/tm2 females appeared normal until 4 mo of age (Figure S4G). Unlike the oocyte-specfic cKO mouse lines, ovulation rates of hormonally stimulated pre-pubertal Mll2tm2/tm2 and control mice were comparable (Mll2tm2/+: 42.0±3.0; Mll2tm2/tm2:34.0±5; n = 4). Furthermore, fertilization rates of young control and Mll2tm2/tm2 females were also comparable (WT: 30.0±2.7; Mll2tm2/+: 28.0±2.7; Mll2tm2/tm2: 29.4±3.9; n = 12). These findings suggested that subfertility in Mll2tm2/tm2 females was due to embryonic defects. In embryo cultures, most embryos from Mll2tm2/tm2 intercrosses (75%) arrested between the 1-cell and 4-cell stages whereas >70% of WT embryos developed to the blastocyst stage regardless of the male genotype (Figure S4H). This phenotype was very similar to that of embryos obtained from crossing Mll2Zp3cKO females to WT males. Additionally, a higher percentage of fragmentation (22%), which indicates decreased survival, was observed in Mll2tm2/tm2 embryos compared to controls (5%). The severity of this outcome was slightly relieved when Mll2tm2/tm2 females were crossed to WT males (Figure S4I), indicating that expression of Mll2 from the paternal allele could partially rescue the defect. Development was also impaired when Mll2tm2/+ females were mated to Mll2tm2/tm2 or even WT males (Figure S4H and S4I).
Mll2tm2/tm2 Embryos Show Both Reduced ZGA and Bulk H3K4 Methylation
Because the majority of the Mll2tm2/tm2 embryos arrested before the 8-cell stage, we evaluated their ability to undergo ZGA using synthesis of the Transcription-Requiring Complex  as a marker. Embryos from Mll2tm2/tm2 intercrosses showed a 30% reduction in TRC levels compared to controls (Figure S5A and S5B; n = 3, p<0.05). Therefore, defective ZGA likely contributes to the developmental block observed in Mll2tm2/tm2 embryos.
To investigate the potential link between MLL2 activity and ZGA, we analyzed the status of H3K4 methylation in Mll2tm2/tm2 embryos. Immunofluorescence and confocal microscopy (Figure S6) showed decreased levels of H3K4me2 and H3K4me3 in female pronuclei of 1-cell embryos from Mll2tm2/tm2 intercrosses (Figure S6B and S6C), whereas control embryos showed the expected asymmetry for H3K4 methylation between the female and male pronuclei (Figure S6B abd S6C) . Western blot analysis of chromatin fractions and chemoluminescence quantification confirmed a significant reduction in H3K4me3 levels in Mll2tm2/tm2 zygotes (Figure S6, left and right panels). The results from the hypomorphic allele are in agreement with and extend the results from conditional mutagenesis of Mll2. Near-ubiquituous mutagenesis of Mll2 in female adults caused infertility (Figure S1). Removal of Mll2 from oocytes but not from granulosa cells also caused infertility. Gdf9-cre-mediated deletion of Mll2 caused a more severe phenotype than Zp3-cre-mediated deletion, consistent with the earlier expression of Gdf9 in oocytes. The hypomorphic allele of Mll2 presented the mildest phenotype, consistent with a reduction, but not abolishment of MLL2 protein levels (Figure S4E).
In this article, we identify MLL2 as one of the major factors controlling bulk H3K4 methylation during oocyte growth and pre-implantation development. Our results indicate that MLL2 is autonomously required in the oocyte for normal oogenesis and pre-implantation development. Oocyte-specific cKO females showed severe defects in fertility, which were also observed in both conditionally mutated and hypomorphic adult females, wheras granulosa cell-specific cKO females showed almost normal fertility. The results suggest differential regulation in H3K4 methylation in gametes and somatic cells. Unlike cKO oocytes (Table 1), Mll2Amhr2 cKO granulosa cells showed an increase in Mll3, which could compensate, at least partially, for the loss of Mll2.
Our studies demonstrate that MLL2 is required during the transcriptionally active period of oocyte growth for the establishment and/or maintenance of bulk H3K4 tri-methylation (Figure 9). A previous study showed that H3K4 methylation increases from immature (meiotically incompetent) oocytes to peri-ovulatory stage (meiotically competent) oocytes . That study also showed that the expression of the H3K4 methyltransferases Mll1 and Smyd3 decreased towards the peri-ovulatory stage, suggesting that a different methyltransferase is responsible for H3K4 methylation in peri-ovulatory oocytes. Here, we show that Mll2 expression increases towards the peri-ovulatory stage and that its loss in oocytes leads to decreased H3K4 methylation. Our results are consistent with the idea that MLL2 is responsible for the increase in H3K4 methylation in peri-ovulatory oocytes observed by Kageyama and collaborators. In addition, the results suggest that although MLL2 is required throughout oogenesis, MLL2 might be particularly important as oocytes develop meiotic competence.
During normal folliculogenesis, oocytes are transcriptionally active and grow in size but do not proliferate. In contrast, the surrounding somatic cells are actively dividing. A number of epigenetic marks are acquired by oocytes during folliculogenesis, including DNA methylation and histone tail modifications. Towards the peri-ovulatory stage, oocytes undergo a large chromatin re-arrangenment into the surrounded nucleolus (SN) configuration and global transcriptional repression (TR). Upon ovulation and fertilization, the maternal pronucleus retains the majority of the histone tail modification marks established in the oocyte, whereas the male pronucleus acquires them progressively. Between the late 1-cell and the 2-cell embryo stages, the maternal mRNA and protein stores are depleted and the zygotic genome is activated (ZGA). MLL2 is expressed in oocytes and during pre-implantation development. Mll2 oocyte-specific cKO females are infertile due to anovulation and follicle loss. Mll2-deficient oocytes show reduced H3K4me3 but not H3K4me1, indicating another enzyme monomethylates H3K4 in oocytes. Oocytes lacking Mll2 also show abnormal expression of pro-apoptotic genes and Iap elements, which may contribute to oocyte death and, ultimately, follicle loss. Although Mll2-deficient oocytes display the SN configuration, they fail to repress transcription, likely due to loss of communication with the granulosa cell compartment. Finally, studies in the hypomorph and the Mll2Zp3 cKO model reveal that MLL2 is required either directly or indirectly for embryogenesis and ZGA. Together the results suggest that MLL2 is continuously required during oogenesis and early embryonic development.
Changes in bulk H3K4me3 methylation (Figures 5, 7, and 9) observed in oocytes were unexpected because loss of Mll2 in ES cells or adult mice did not affect bulk H3K4me3 ,. Our findings demonstrate that MLL2 is responsible for the majority of H3K4me3 in oocytes and that a different enzyme(s) is responsible for H3K4me1. Mono-methylation by dedicated monomethylases that co-operate with dedicated di-/tri-methylases has been observed for other histone tail lysines but not for H3K4 . Until now, there has been no evidence that a similar mechanism may operate on mammalian H3K4 methylation , thereby differing from the paradign H3K4 methyltransferase, yeast Set1C, which mediates mono-, di-, and tri-methylation under the control of its various subunits ,,.
Oocytes lacking Mll2 displayed a variety of defects including lack of global transcriptional silencing at the peri-ovulatory stage and abnormal expression of Uhfr1, Ccnblip1, Iap, and pro-apoptotic factors (Table 1 and Figures S2, S3, and 9). The failure to undergo transcriptional silencing is paradoxical because MLL2, like other trithorax-Group factors, acts to maintain expression of target genes . A possible explanation for this paradox is that MLL2 is required for expression of key factors that mediate global transcriptional silencing, and therefore, this defect is likely to be an indirect consequence of Mll2 loss. Previous studies have shown that granulosa cell-oocyte communication is essential for transcriptional repression in oocytes . Thus, it is possible that altered communication between the gamete and somatic cell compartments in Mll2Gdf9 cKO ovaries, as evidenced by the ovulatory defect, may contribute to the lack of transcriptional repression. A previous study showed that global transcriptional silencing occurred without the large chromatin rearrangement into the SN configuration . Here we extend this observation by showing the converse: acquisition of the SN configuration in Mll2-deficient oocytes occurred without global transcriptional silencing, confirming that these two events are independent.
It is likely that the observed overexpression of Iap elements and apoptotic factors in Mll2-deficient oocytes is an indirect consequence of Mll2 loss. In contrast, the decrease in the expression of Uhfr1 and Ccnblip1 may reflect direct regulation of these genes by MLL2, since both genes are expressed from CpG islands, as are all MLL2 targets described to date .
Results in the hypomorph and Mll2Zp3 cKO models demonstrated a developmental arrest between the 1- and 4-cell stages, when ZGA takes place in mouse embryos. In addition, Mll2 hypomorph zygotes displayed impaired ZGA (Figure 9). A possible explanation for this phenotype is that unknown maternal factors required for ZGA are lacking in Mll2-deficient oocytes. Another possibility is that the establishment of H3K4 di- and tri-methylation in the oocyte by MLL2 contributes to establish a poised starting point for the activation of the embryonic genome after fertilization. Finally, it is also possible that MLL2 itself might be required to activate transcription in the zygote. In this context, we note that MLL2 appears to be continuously required after fertilization during at least the first three cell divisions (Figure 9). This conclusion is supported by the fact that MLL2 is expressed in pre-implantation embryos from the 2-cell stage onwards and that embryos derived from Mll2Zp3 cKO and Mll2tm2/tm2 females undergo developmental arrest. Recently, an unidentified MLL protein has been implicated as part of a BRG-1 containing complex required for ZGA . Our results from the hypomorph model suggest that MLL2 could be this protein.
Finally, recent studies have shown that H3K4 histone methyltransferases, including MLL2, may also play a role in nuclear receptor activation and hormone signaling, at least in human cells . Thus, defective signaling pathways could also contribute to the phenotype observed in the various Mll2 cKO mouse lines. Undoubtely, more studies are needed to dissect the potential role of MLL2 in those pathways.
Together with previous studies , our findings suggest that during development there is a switch from an MLL2-dependent to MLL2-independent state. In the oocyte, MLL2 is the major tri-H3K4 methyltransferase, and maternal MLL2 is required for the first embryonic divisions. Previous studies showed that Mll2 KO mice develop up to E9.5 , suggesting that an MLL2-independent state is established after ZGA. We hypothesize that a cell-type specific requirement for MLL2 may be established later in development during gastrulation. This implies a cell-type specific switch in epigenetic roles, which points to a new dimension of complexity in epigenetic regulation.
Our results indicate that MLL2 is required during post-natal oogenesis and the 2–3 embryonic cell divisions, thereby identifying MLL2 as one of the key players in the epigenetic reprogramming required for female fertility in the mouse. Given the recent interest in epigenetics raised by the reprogramming of somatic cells to a pluripotent state equivalent to the epiblast, termed iPS (induced pluripotent stem cells), it will be interesting to determine whether MLL2 is also required for the generation of iPS. If this holds true, MLL2 dependency could be used as a criterion to determine whether reprogramming to iPS cells recapitulates endogenous mechanisms or is largely synthetic short-circuiting.
Materials and Methods
Generation of Mouse Lines
Conditional KO mice were generated by crossing Rosa26CreERT2  Gdf9-Cre , zona pellucida 3 (Zp3)-Cre , or anti-Mullerian hormone receptor 2 (Amhr2)-Cre  mice and Mll2+/− and Mll2F/F mice  as explained in Text S1. The hypomorphic line Mll2tm2 was generated inadvertently when a yellow fluorescence protein (YFP) tag was added in frame with the first ATG of Mll2 located in exon 1 (Figure 1A, Text S1). All mouse lines were maintained in the 129/C57BL/6 hybrid background. Genotyping from tail DNA was performed as described ,,. The decrease in Mll2 levels was confirmed by quantitative PCR (QPCR) analysis using primers described in Table S1. Mice were maintained in accordance with the NIH Guide for the Care and Use of Laboratory Animals and under German license nr. 24-9168.11-1-2004-26.
Fertility Studies, Serum Hormone Levels, Histological Analysis, Cell Collection, and Culture
Fertility studies, serum hormone measurements, and histological analysis were performed as previously described . More details are provided in Text S1. 21-d-old females were injected with 5 IU of pregnant mare serum gonadotropin (PMSG; Calbiochem, EMD, Gibbstown, NJ), and granulosa cells or peri-ovulatory oocytes were collected 47 h later . Superovulation and ovulation rates in 21-d-old females were carried out as described . In vitro oocyte maturation and embryo cultures are detailed in Text S1.
RT-PCR and Quantitative Real Time PCR (QPCR) Analyses
RNA from oocytes or granulosa cells was isolated and subjected to QPCR analysis as described in Text S1. Primer sets are described in  and Table S1. Primer amplification efficiency and transcript levels were calculated as previously described . Gapdh was used as endogenous control. The relative amount of target gene expression for each sample was presented as the mean ± SEM.
Chromatin Isolation and Western Blot Analysis
Chromatin was isolated from oocytes or granulosa cells without nuclease treatment  and equal amounts of protein or equal numbers of oocytes were fractionated in 4%–12% SDS-PAGE Bis-Tris gels (Invitrogen, Carlsbad, CA). Total histones were determined by SYPRO Ruby protein blot stain (Molecular Probes, Invitrogen). Proteins were detected with Super-signal West Pico-detection system (Pierce/Thermo Fisher Scientific, Pittsburgh, PA), and levels were quantified by using the Image J software (NIH). Results are presented as a ratio of modified histone to total histones or protein to tubulin and represent the average of three pools collected in three experiments.
Immunofluorescence and Confocal Laser-Scanning Microscopy
Peri-ovulatory oocytes were fixed and stained as previously described . Samples were mounted in well-slides (Fisher Scientific) using Vectashield containing propidium iodine (Vector Laboratories, Burlingame, CA). Fluorescence was detected on a laser-scanner confocal microscope (Carl Zeiss, Thornwood, NY). Images were collected at 40× magnification with zoom level equal to 2. For each experimental series, oocytes from all groups were processed under the same conditions and images were captured using the same microscope settings.
Metabolic Labeling, TRC Complex Detection, Run-On Assays, and DNA Methylation Assays
Metabolic labeling of 2-cell embryos, protein extraction, and TRC detection were performed as previously reported ,. Transcriptional activity was determined in run-on studies after 5-bromo uridine 5′-triphosphate (BrUTP; Sigma) incorporation as previously described ,. A detailed explanation is presented in Text S1. Global DNA methylation was assessed by immunostaining against 5-methylcytosine, and Iap DNA methylation was determined by bisulfite genomic sequencing  or bisulphite conversion followed by PCR analysis using methylation-specific primers, which were designed using MethPrimer.
Loss of Mll2 in adult females using tamoxifen-induced Cre recombination leads to infertility. Mll2F/F; Rosa26CreERT2/+ (Mll2F, conditional KO, grey bars) or Mll2F/+; Rosa26CreERT2/+ (control, black bars) females (upper panel) were mated to WT males. Initially ten 2-mo-old adults of each genotype were mated to establish fertility (before induction), then they were treated with tamoxifen as described  and tested for fertility. The WT males (one male for each pair of tamoxifen treated females) were exchanged every week. Data show the number of successful litters. Note that tamoxifen treatment provokes infertility in females regardless of genotype. After treatment was discontinued, control animals fully recover fertility, whereas Mll2 conditional KO females remain infertile.
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Mll2Gdf9 cKO oocytes show increased expression of the methyltransferase Setd7, p53 stabilization, and expression of p53 downstream pro-apoptotic target genes. (A) QPCR analysis showed a significant increase in the methyltransferase Setd7 in Mll2Gdf9 cKO peri-ovulatory oocytes (Student's t test, * p<0.05; three pools of oocytes were used in the analysis (n = 3)). Gapdh was used as endogenous control. (B) Representative Western blot analysis showing increased p53 protein levels in Mll2Gdf9 cKO peri-ovulatory oocytes; tubulin was used as loading control. Note that p53 is normally absent in peri-ovulatory stage oocytes. (C) QPCR analysis showed a significant increase in the expression of p53 target genes,which are involved in apoptosis, including Bax, Cdkn1a, Fos, and Casp6 in Mll2Gdf9 cKO peri-ovulatory oocytes (Student's t test, * p<0.05; ** p<0.01; three pools of oocytes were used in the analysis (n = 3)). Gapdh was used as endogenous control.
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Mll2Gdf9 cKO oocytes display abnormal levels of the retrotransposable element Iap. (A) QPCR analysis of retrotransposon mRNA in isolated peri-ovulatory Mll2Gdf9cKO oocytes showed an increase (Student's t test, * p<0.05) in Iap (intracisternal A particle) but not in LINE-1 (Long Interspersed Nuclear Element 1, L1) or SINE-1 (Short Interspersed Nuclear Element); means ± S.E. are shown (three pools of 100 oocytes each were used in the analysis; n = 3). (B) Methylation-specific PCR analysis of the Iap promoter showed a significant decrease in CpG DNA methylation in peri-ovulatory Mll2Gdf9 cKO oocytes (Student's t test, * p<0.05); means ± S.E. are shown (three pools of 100 oocytes each were used in the analysis; n = 3). (C) Hypomethylation of Iap in peri-ovulatory Mll2Gdf9 cKO oocytes was confirmed by bisulphite sequencing. Methylated and unmethylated CpGs are shown as filled or open circles, respectively. (D) 5-methylcytosine (5-Me-C) staining and confocal microscopy analysis reveal that loss of DNA methylation in peri-ovulatory Mll2Gdf9 cKO oocytes is not a widespread phenomenon. Merge and grayscale split channels (5-Me-C, FITC; DNA, propidium iodide, red) of single plane confocal sections are shown. Magnification: 800×.
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Generation and characterization of Mll2tm2afst/tm2afst ((Mll2tm2/tm2) mice. (A) Schematic representation of targeted mutation 2 Mll2tm2afst (denoted throughout as Mll2tm2). An enhanced yellow fluorescence protein (eYFP) cassette was introduced in the N terminus end of Mll2, followed by a neomycin (neo) cassette flanked by two Frt (FLP recombination target) sites. FLPe (Flip)-mediated recombination resulted in a continuous reading frame from the authentic Mll2 initiating codon, through eYFP and the residual FRT to the second amino acid of Mll2. (B) Mice carrying a single copy of Mll2tm2 (Mll2tm2/+) were intercrossed to obtain control (Mll2 +/+and Mll2tm2/+) and experimental Mll2tm2/tm2 mice. (C) Southern blot analysis of tail genomic DNA: a 3.8 kb band denotes the Mll2tm2/tm2 allele, whereas a 3.0 kb band denotes the wild type (WT) allele. Mll2tm2/+ mice are distinguished by the presence of the two bands. (D) Real time PCR analysis showing a small but significant decrease in Mll2 mRNA levels in ovaries from Mll2tm2/tm2 females (ovaries from three females were used in the analysis (n = 3); Student's t test, p<0.05). (E) Representative Western blot analysis showing a decrease in MLL2 levels in ovaries from Mll2tm2/tm2 females; tubulin was used as loading control. (F) Fertility studies shown as cumulative number of pups over a 6-mo period. Females were mated to WT males. The cumulative number of pups produced by Mll2tm2/tm2 females was lower than that of controls indicating subfertility (n = 10 per genotype). (G) PAS-stained ovaries from 3-wk-old mice. Mll2tm2/tm2 ovaries show normal follicular development at this age. Original magnification: 50×. (H,I) Developmental potential of Mll2tm2/tm2 embryos. Pre-pubertal (21-d-old) females were superovulated and mated to 6-wk-old Mll2tm2/tm2 males, which showed no defects in fertility at this age (H) or WT males (I). Embryonic development was evaluated in vitro. Means ± S.E. from five independent experiments are shown (approximately 35 embryos per each female, from a total of 8–11 females per genotype, were used in the analysis). (H) When females were mated to Mll2tm2/tm2 males, a significantly lower percentage of Mll2tm2/tm2 embryos reached the blastocyst stage; embryos accumulated between 1C and 4C stages. In contrast, the majority of embryos from WT females developed to blastocysts. (I) When females were mated to WT males, few embryos from Mll2tm2/tm2 females reached the blastocyst stage. Abbreviations: 1C, 1-cell; 2C, 2-cell; M, morula; Bl, blastocyst. ANOVA test; * p<0.05.
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Mll2tm2/tm2 embryos show defects in zygote genome activation (ZGA). (A) Representative autoradiogram showing Transcription Required Complex (TRC) complex levels. Two-cell embryos underwent metabolic labeling to determine TRC synthesis, as a marker of ZGA. Note the reduction in TRC levels in Mll2tm2/tm2 2-cell embryos (Student's t test, * p<0.05) in Mll2tm2/tm2 2-cell embryo pools (three pools of embryos from six females per genotype were used in this experiment; n = 3); α-amanitin-(α-a)-treated embryos were used as negative controls. (B) Quantification of TRC complex levels. TRC was significantly reduced (Student's t test, * p<0.05) in Mll2tm2/tm2 2-cell embryo pools (three pools of embryos from six females per genotype were used in this experiment; n = 3).
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Mll2tm2/tm2 embryos show defects in H3K4 bulk methylation. (A–C) Representative micrographs showing confocal microscopy analysis of H3K4me1(A), H3K4me2 (B), and H3K4me3 (C); a decrease in H3K4 di- and tri-methylation was observed in the female (f) pronucleus of Mll2tm2/tm2 embryos; the male pronucleus (m) is negative for H3K4me2 and H3K4me3, as expected; polar bodies (PB) stain with all antibodies used, as previously reported. Merge and grayscale split channels (H3K3 methylation, FITC; DNA, propidium iodide, red) of single plane confocal sections are shown. (D–E) Western blot analysis of H3K4 methylation in chromatin fractions from zygotes. Three pools of 100 zygotes each from 3–4 females per genotype were used in three independent experiments. Total histones were used as internal loading control. (D) Representative micrographs of Western blots showing H3K4me1 and H3K4me3 levels. (E) Chemoluminescence quantification revealed a significant decrease in global H3K4me3 levels in Mll2tm2/tm2 embryos; samples were normalized against total histones. Student's t test, * p<0.05.
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List of primer sequences.
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Supplementary materials and methods.
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The authors wish to thank Dr. A. Cooney (Baylor College of Medicine) for supplying the Gdf9-Cre and Dr. R. Behringer (M.D. Anderson) for supplying the Amhr2-Cre mouse line, Dr. J. Schaft and Dr. K. Burns for early work on the Mll2tm2/tm2 allele mice, and Dr. D. Vieyra for critical reading of the manuscript.
The author(s) have made the following declarations about their contributions: Conceived and designed the experiments: CVAV SG KA AFS MMM. Performed the experiments: CVAV SG KA. Analyzed the data: CVAV. Contributed reagents/materials/analysis tools: AFS MMM. Wrote the paper: CVAV AFS. Revised the manuscript: CVAV MMM. Maintenance of mouse colonies/genotyping: RC JEA. Performed studies in the Tamoxifen-Cre line and preliminary studies in Mll2 hypomorph; designed original Mll2 KO and Floxed alleles: SG KA.
- 1. De La Fuente R, Viveiros M. M, Burns K. H, Adashi E. Y, Matzuk M. M, et al. (2004) Major chromatin remodeling in the germinal vesicle (GV) of mammalian oocytes is dispensable for global transcriptional silencing but required for centromeric heterochromatin function. Dev Biol 275: 447–458.
- 2. Reik W, Santos F, Mitsuya K, Morgan H, Dean W (2003) Epigenetic asymmetry in the mammalian zygote and early embryo: relationship to lineage commitment? Philos Trans R Soc Lond B Biol Sci 358: 1403–1409; discussion 1409.
- 3. Farthing C. R, Ficz G, Ng R. K, Chan C. F, Andrews S, et al. (2008) Global mapping of DNA methylation in mouse promoters reveals epigenetic reprogramming of pluripotency genes. PLoS Genet 4: e1000116. doi:10.1371/journal.pbio.1000116.
- 4. Silva J, Nichols J, Theunissen T. W, Guo G, van Oosten A. L, et al. (2009) Nanog is the gateway to the pluripotent ground state. Cell 138: 722–737.
- 5. Nichols J, Smith A (2009) Naive and primed pluripotent states. Cell Stem Cell 4: 487–492.
- 6. Matzuk M. M, Lamb D. J (2002) Genetic dissection of mammalian fertility pathways. Nat Cell Biol 4: Suppls41–49.
- 7. Edson M. A, Nagaraja A. K, Matzuk M. M (2009) The Mammalian ovary from genesis to revelation. Endocr Rev 30: 624–712.
- 8. Schultz R. M (2002) The molecular foundations of the maternal to zygotic transition in the preimplantation embryo. Hum Reprod Update 8: 323–331.
- 9. Liu H, Aoki F (2002) Transcriptional activity associated with meiotic competence in fully grown mouse GV oocytes. Zygote 10: 327–332.
- 10. Bouniol-Baly C, Hamraoui L, Guibert J, Beaujean N, Szollosi M. S, et al. (1999) Differential transcriptional activity associated with chromatin configuration in fully grown mouse germinal vesicle oocytes. Biol Reprod 60: 580–587.
- 11. Debey P, Szollosi M. S, Szollosi D, Vautier D, Girousse A, et al. (1993) Competent mouse oocytes isolated from antral follicles exhibit different chromatin organization and follow different maturation dynamics. Mol Reprod Dev 36: 59–74.
- 12. De La Fuente R, Eppig J. J (2001) Transcriptional activity of the mouse oocyte genome: companion granulosa cells modulate transcription and chromatin remodeling. Dev Biol 229: 224–236.
- 13. Zuccotti M, Ponce R. H, Boiani M, Guizzardi S, Govoni P, et al. (2002) The analysis of chromatin organisation allows selection of mouse antral oocytes competent for development to blastocyst. Zygote 10: 73–78.
- 14. De La Fuente R (2006) Chromatin modifications in the germinal vesicle (GV) of mammalian oocytes. Dev Biol 292: 1–12.
- 15. Schaefer C. B, Ooi S. K, Bestor T. H, Bourc'his D (2007) Epigenetic decisions in mammalian germ cells. Science 316: 398–399.
- 16. Lucifero D, Mertineit C, Clarke H. J, Bestor T. H, Trasler J. M (2002) Methylation dynamics of imprinted genes in mouse germ cells. Genomics 79: 530–538.
- 17. Lucifero D, Mann M. R, Bartolomei M. S, Trasler J. M (2004) Gene-specific timing and epigenetic memory in oocyte imprinting. Hum Mol Genet 13: 839–849.
- 18. Bourc'his D, Xu G. L, Lin C. S, Bollman B, Bestor T. H (2001) Dnmt3L and the establishment of maternal genomic imprints. Science 294: 2536–2539.
- 19. Reik W, Dean W, Walter J (2001) Epigenetic reprogramming in mammalian development. Science 293: 1089–1093.
- 20. Tanaka M, Kihara M, Hennebold J. D, Eppig J. J, Viveiros M. M, et al. (2005) H1FOO is coupled to the initiation of oocytic growth. Biol Reprod 72: 135–142.
- 21. Tanaka M, Kihara M, Meczekalski B, King G. J, Adashi E. Y (2003) H1oo: a pre-embryonic H1 linker histone in search of a function. Mol Cell Endocrinol 202: 5–9.
- 22. Kageyama S, Liu H, Kaneko N, Ooga M, Nagata M, et al. (2007) Alterations in epigenetic modifications during oocyte growth in mice. Reproduction 133: 85–94.
- 23. Kouzarides T (2007) Chromatin modifications and their function. Cell 128: 693–705.
- 24. Kundu S, Peterson C. L (2009) Role of chromatin states in transcriptional memory. Biochim Biophys Acta 1790: 445–455.
- 25. Schultz R. M, Worrad D. M (1995) Role of chromatin structure in zygotic gene activation in the mammalian embryo. Semin Cell Biol 6: 201–208.
- 26. Thompson E. M, Legouy E, Renard J. P (1998) Mouse embryos do not wait for the MBT: chromatin and RNA polymerase remodeling in genome activation at the onset of development. Dev Genet 22: 31–42.
- 27. Li L, Zheng P, Dean J (2010) Maternal control of early mouse development. Development 137: 859–870.
- 28. van der Heijden G. W, Dieker J. W, Derijck A. A, Muller S, Berden J. H, et al. (2005) Asymmetry in histone H3 variants and lysine methylation between paternal and maternal chromatin of the early mouse zygote. Mech Dev 122: 1008–1022.
- 29. Lepikhov K, Walter J (2004) Differential dynamics of histone H3 methylation at positions K4 and K9 in the mouse zygote. BMC Dev Biol 4: 12.
- 30. Santos F, Hendrich B, Reik W, Dean W (2002) Dynamic reprogramming of DNA methylation in the early mouse embryo. Dev Biol 241: 172–182.
- 31. Sarmento O. F, Digilio L. C, Wang Y, Perlin J, Herr J. C, et al. (2004) Dynamic alterations of specific histone modifications during early murine development. J Cell Sci 117: 4449–4459.
- 32. O'Carroll D, Erhardt S, Pagani M, Barton S. C, Surani M. A, et al. (2001) The polycomb-group gene Ezh2 is required for early mouse development. Mol Cell Biol 21: 4330–4336.
- 33. Erhardt S, Su I. H, Schneider R, Barton S, Bannister A. J, et al. (2003) Consequences of the depletion of zygotic and embryonic enhancer of zeste 2 during preimplantation mouse development. Development 130: 4235–4248.
- 34. Mager J, Montgomery N. D, de Villena F. P, Magnuson T (2003) Genome imprinting regulated by the mouse Polycomb group protein Eed. Nat Genet 33: 502–507.
- 35. Jenuwein T (2006) The epigenetic magic of histone lysine methylation. Febs J 273: 3121–3135.
- 36. Roguev A, Schaft D, Shevchenko A, Pijnappel W. W, Wilm M, et al. (2001) The Saccharomyces cerevisiae Set1 complex includes an Ash2 homologue and methylates histone 3 lysine 4. Embo J 20: 7137–7148.
- 37. Glaser S, Schaft J, Lubitz S, Vintersten K, van der Hoeven F, et al. (2006) Multiple epigenetic maintenance factors implicated by the loss of Mll2 in mouse development. Development 133: 1423–1432.
- 38. Milne T. A, Briggs S. D, Brock H. W, Martin M. E, Gibbs D, et al. (2002) MLL targets SET domain methyltransferase activity to Hox gene promoters. Mol Cell 10: 1107–1117.
- 39. Glaser S, Lubitz S, Loveland K. L, Ohbo K, Robb L, et al. (2009) The histone 3 lysine 4 methyltransferase, Mll2, is only required briefly in development and spermatogenesis. Epigenetics Chromatin 2: 5.
- 40. Lan Z. J, Xu X, Cooney A. J (2004) Differential oocyte-specific expression of Cre recombinase activity in GDF-9-iCre, Zp3cre, and Msx2Cre transgenic mice. Biol Reprod 71: 1469–1474.
- 41. Lewandoski M, Wassarman K. M, Martin G. R (1997) Zp3-cre, a transgenic mouse line for the activation or inactivation of loxP-flanked target genes specifically in the female germ line. Curr Biol 7: 148–151.
- 42. Jamin S. P, Arango N. A, Mishina Y, Hanks M. C, Behringer R. R (2003) Genetic studies of the AMH/MIS signaling pathway for Mullerian duct regression. Mol Cell Endocrinol 211: 15–19.
- 43. Chang Y. F, Imam J. S, Wilkinson M. F (2007) The nonsense-mediated decay RNA surveillance pathway. Annu Rev Biochem 76: 51–74.
- 44. Nilsson E, Skinner M. K (2001) Cellular interactions that control primordial follicle development and folliculogenesis. J Soc Gynecol Investig 8: S17–20.
- 45. Ross P. J, Ragina N. P, Rodriguez R. M, Iager A. E, Siripattarapravat K, et al. (2008) Polycomb gene expression and histone H3 lysine 27 trimethylation changes during bovine preimplantation development. Reproduction 136: 777–785.
- 46. Levey I. L, Brinster R. L (1978) Effects of alpha-amanitin on RNA synthesis by mouse embryos in culture. J Exp Zool 203: 351–360.
- 47. Volkel P, Angrand P. O (2007) The control of histone lysine methylation in epigenetic regulation. Biochimie 89: 1–20.
- 48. Ruthenburg A. J, Allis C. D, Wysocka J (2007) Methylation of lysine 4 on histone H3: intricacy of writing and reading a single epigenetic mark. Mol Cell 25: 15–30.
- 49. Bernstein B. E, Mikkelsen T. S, Xie X, Kamal M, Huebert D. J, et al. (2006) A bivalent chromatin structure marks key developmental genes in embryonic stem cells. Cell 125: 315–326.
- 50. Bernstein B. E, Kamal M, Lindblad-Toh K, Bekiranov S, Bailey D. K, et al. (2005) Genomic maps and comparative analysis of histone modifications in human and mouse. Cell 120: 169–181.
- 51. Arney K. L, Bao S, Bannister A. J, Kouzarides T, Surani M. A (2002) Histone methylation defines epigenetic asymmetry in the mouse zygote. Int J Dev Biol 46: 317–320.
- 52. Kim J. M, Liu H, Tazaki M, Nagata M, Aoki F (2003) Changes in histone acetylation during mouse oocyte meiosis. J Cell Biol 162: 37–46.
- 53. Yu J, Deng M, Medvedev S, Yang J, Hecht N. B, et al. (2004) Transgenic RNAi-mediated reduction of MSY2 in mouse oocytes results in reduced fertility. Dev Biol 268: 195–206.
- 54. Zheng P, Dean J (2007) Oocyte-specific genes affect folliculogenesis, fertilization, and early development. Semin Reprod Med 25: 243–251.
- 55. Ye Y, Kawamura K, Sasaki M, Kawamura N, Groenen P, et al. (2009) Kit ligand promotes first polar body extrusion of mouse preovulatory oocytes. Reprod Biol Endocrinol 7: 26.
- 56. Reddy P, Liu L, Adhikari D, Jagarlamudi K, Rajareddy S, et al. (2008) Oocyte-specific deletion of Pten causes premature activation of the primordial follicle pool. Science 319: 611–613.
- 57. Masciarelli S, Horner K, Liu C, Park S. H, Hinckley M, et al. (2004) Cyclic nucleotide phosphodiesterase 3A-deficient mice as a model of female infertility. J Clin Invest 114: 196–205.
- 58. McMahon H. E, Hashimoto O, Mellon P. L, Shimasaki S (2008) Oocyte-specific overexpression of mouse bone morphogenetic protein-15 leads to accelerated folliculogenesis and an early onset of acyclicity in transgenic mice. Endocrinology 149: 2807–2815.
- 59. Hutt K. J, McLaughlin E. A, Holland M. K (2006) KIT/KIT ligand in mammalian oogenesis and folliculogenesis: roles in rabbit and murine ovarian follicle activation and oocyte growth. Biol Reprod 75: 421–433.
- 60. Bultman S. J, Gebuhr T. C, Pan H, Svoboda P, Schultz R. M, et al. (2006) Maternal BRG1 regulates zygotic genome activation in the mouse. Genes Dev 20: 1744–1754.
- 61. Tong Z. B, Gold L, Pfeifer K. E, Dorward H, Lee E, et al. (2000) Mater, a maternal effect gene required for early embryonic development in mice. Nat Genet 26: 267–268.
- 62. Oda H, Okamoto I, Murphy N, Chu J, Price S. M, et al. (2009) Monomethylation of histone H4-lysine 20 is involved in chromosome structure and stability and is essential for mouse development. Mol Cell Biol 29: 2278–2295.
- 63. Payer B, Saitou M, Barton S. C, Thresher R, Dixon J. P, et al. (2003) Stella is a maternal effect gene required for normal early development in mice. Curr Biol 13: 2110–2117.
- 64. Ward J. O, Reinholdt L. G, Motley W. W, Niswander L. M, Deacon D. C, et al. (2007) Mutation in mouse hei10, an e3 ubiquitin ligase, disrupts meiotic crossing over. PLoS Genet 3: e139. doi:10.1371/journal.pgen.0030139.
- 65. Oliveri R. S, Kalisz M, Schjerling C. K, Andersen C. Y, Borup R, et al. (2007) Evaluation in mammalian oocytes of gene transcripts linked to epigenetic reprogramming. Reproduction 134: 549–558.
- 66. Chuikov S, Kurash J. K, Wilson J. R, Xiao B, Justin N, et al. (2004) Regulation of p53 activity through lysine methylation. Nature 432: 353–360.
- 67. Ghafari F, Pelengaris S, Walters E, Hartshorne G. M (2009) Influence of p53 and genetic background on prenatal oogenesis and oocyte attrition in mice. Hum Reprod 24: 1460–1472.
- 68. Elkeles A, Juven-Gershon T, Israeli D, Wilder S, Zalcenstein A, et al. (1999) The c-fos proto-oncogene is a target for transactivation by the p53 tumor suppressor. Mol Cell Biol 19: 2594–2600.
- 69. Andreu-Vieyra C, Chen R, Matzuk M. M (2007) Effects of granulosa cell-specific deletion of Rb in Inha-alpha null female mice. Endocrinology 148: 3837–3849.
- 70. MacLachlan T. K, El-Deiry W. S (2002) Apoptotic threshold is lowered by p53 transactivation of caspase-6. Proc Natl Acad Sci U S A 99: 9492–9497.
- 71. Arnault E, Doussau M, Pesty A, Lefevre B, Courtot (2010) AM Review: Lamin A/C, caspase-6, and chromatin configuration during meiosis resumption in the mouse oocyte. Reprod Sci 17: 102–115.
- 72. Peaston A. E, Evsikov A. V, Graber J. H, de Vries W. N, Holbrook A. E, et al. (2004) Retrotransposons regulate host genes in mouse oocytes and preimplantation embryos. Dev Cell 7: 597–606.
- 73. Sharif J, Muto M, Takebayashi S, Suetake I, Iwamatsu A, et al. (2007) The SRA protein Np95 mediates epigenetic inheritance by recruiting Dnmt1 to methylated DNA. Nature 450: 908–912.
- 74. Kim J. K, Esteve P. O, Jacobsen S. E, Pradhan S (2009) UHRF1 binds G9a and participates in p21 transcriptional regulation in mammalian cells. Nucleic Acids Res 37: 493–505.
- 75. Schultz R. M (1993) Regulation of zygotic gene activation in the mouse. Bioessays 15: 531–538.
- 76. Lubitz S, Glaser S, Schaft J, Stewart A. F, Anastassiadis K (2007) Increased apoptosis and skewed differentiation in mouse embryonic stem cells lacking the histone methyltransferase Mll2. Mol Biol Cell 18: 2356–2366.
- 77. Wu X, Hua X (2008) Menin, histone h3 methyltransferases, and regulation of cell proliferation: current knowledge and perspective. Curr Mol Med 8: 805–815.
- 78. Shilatifard A (2008) Molecular implementation and physiological roles for histone H3 lysine 4 (H3K4) methylation. Curr Opin Cell Biol 20: 341–348.
- 79. Dehe P. M, Dichtl B, Schaft D, Roguev A, Pamblanco M, et al. (2006) Protein interactions within the Set1 complex and their roles in the regulation of histone 3 lysine 4 methylation. J Biol Chem 281: 35404–35412.
- 80. Ansari K. I, Mandal S. S (2010) Mixed lineage leukemia: roles in gene expression, hormone signaling and mRNA processing. Febs J 277(8): 1790–1804.
- 81. Seibler J, Zevnik B, Kuter-Luks B, Andreas S, Kern H, et al. (2003) Rapid generation of inducible mouse mutants. Nucleic Acids Res 31: e12.
- 82. Andreu-Vieyra C, Chen R, Matzuk M. M (2008) Conditional deletion of the retinoblastoma (Rb) gene in ovarian granulosa cells leads to premature ovarian failure. Mol Endocrinol 22: 2141–2161.
- 83. Mendez J, Stillman B (2000) Chromatin association of human origin recognition complex, cdc6, and minichromosome maintenance proteins during the cell cycle: assembly of prereplication complexes in late mitosis. Mol Cell Biol 20: 8602–8612.
- 84. Cowell I. G, Aucott R, Mahadevaiah S. K, Burgoyne P. S, Huskisson N, et al. (2002) Heterochromatin, HP1 and methylation at lysine 9 of histone H3 in animals. Chromosoma 111: 22–36.
- 85. Conover J. C, Temeles G. L, Zimmermann J. W, Burke B, Schultz R. M (1991) Stage-specific expression of a family of proteins that are major products of zygotic gene activation in the mouse embryo. Dev Biol 144: 392–404.
- 86. Poueymirou W. T, Schultz R. M (1987) Differential effects of activators of cAMP-dependent protein kinase and protein kinase C on cleavage of one-cell mouse embryos and protein synthesis and phosphorylation in one- and two-cell embryos. Dev Biol 121: 489–498.