Wolbachia are vertically transmitted, obligatory intracellular bacteria that infect a great number of species of arthropods and nematodes. In insects, they are mainly known for disrupting the reproductive biology of their hosts in order to increase their transmission through the female germline. In Drosophila melanogaster, however, a strong and consistent effect of Wolbachia infection has not been found. Here we report that a bacterial infection renders D. melanogaster more resistant to Drosophila C virus, reducing the load of viruses in infected flies. We identify these resistance-inducing bacteria as Wolbachia. Furthermore, we show that Wolbachia also increases resistance of Drosophila to two other RNA virus infections (Nora virus and Flock House virus) but not to a DNA virus infection (Insect Iridescent Virus 6). These results identify a new major factor regulating D. melanogaster resistance to infection by RNA viruses and contribute to the idea that the response of a host to a particular pathogen also depends on its interactions with other microorganisms. This is also, to our knowledge, the first report of a strong beneficial effect of Wolbachia infection in D. melanogaster. The induced resistance to natural viral pathogens may explain Wolbachia prevalence in natural populations and represents a novel Wolbachia–host interaction.
Many symbiotic bacteria confer fitness benefits to the organisms that they infect. Wolbachia are one of the most widespread intracellular bacteria, infecting a great number of species of insects. Here we show that in the fruit fly Drosophila melanogaster, infection with Wolbachia increases resistance to a natural pathogen of Drosophila, an RNA virus called Drosophila C virus. Furthermore, we show that Wolbachia also increases resistance of Drosophila to two other RNA viruses (Nora and Flock House virus) but not to a DNA virus (Insect Iridescent Virus 6). These results identify a significant new factor that regulates D. melanogaster resistance to infection by RNA viruses. Our results add to a growing body of literature showing that the response of an organism to a particular pathogen is modulated by prior or contemporaneous interactions with other microorganisms. That the fruit fly clearly benefits from increased resistance to viruses may provide a solution to the longstanding puzzle as to why Wolbachia is so common in natural populations of D. melanogaster.
Citation: Teixeira L, Ferreira Á, Ashburner M (2008) The Bacterial Symbiont Wolbachia Induces Resistance to RNA Viral Infections in Drosophila melanogaster. PLoS Biol 6(12): e1000002. https://doi.org/10.1371/journal.pbio.1000002
Academic Editor: Laurent Keller, University of Lausanne, Switzerland
Received: August 13, 2008; Accepted: November 13, 2008; Published: December 23, 2008
Copyright: © 2008 Teixeira et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: LT was partially supported by a postdoctoral fellowship from Fundação para a Ciência e Tecnologia (Portugal). The research was supported by a grant from the Biotechnology and Biological Sciences Research Council (UK).
Competing interests: The authors have declared that no competing interests exist.
Abbreviations: CI, cytoplasmic incompatibility; DCV, Drosophila C virus; FHV, Flock House virus; IIV-6, Insect Iridescent Virus 6; RT-PCR, reverse-transcription PCR; TCID50, median tissue culture infective dose
Wolbachia are obligatory, intracellular α-proteobacteria that infect a wide range of arthropods and filarial nematodes. They are found in 17% to 76% of surveyed arthropods and have recently been estimated to be present in 66% of all arthropod species [1–3]; therefore Wolbachia are one of the most widespread intracellular bacteria. Although the phylogenies of Wolbachia strains and their arthropod hosts show horizontal transmission of the bacteria on an evolutionary time-scale , these endosymbionts are mainly transmitted maternally. Consequently, Wolbachia strains and the species they infect form long-term associations.
Wolbachia were first discovered infecting the mosquito Culex pipiens in 1924 , but interest in these bacteria mainly arose when it was shown that infected mosquito males do not successfully breed with noninfected females . This phenomenon is termed cytoplasmic incompatibility (CI) and has, since then, been found in many other insect species infected with Wolbachia . In some hosts, Wolbachia can also cause feminization, male killing, or parthenogenesis . All these mechanisms profoundly alter the reproductive biology of their hosts and are thought to increase the success of bacterial transmission through the female germline. In the majority of known cases, Wolbachia behave like reproductive parasites of their hosts.
Interestingly, in the parasitic wasp Asobara tabida, a Wolbachia strain is required for the inhibition of apoptosis in the germline and, consequently, normal oogenesis [8,9]. Similarly, Wolbachia is required for normal development and fertility in many filarial nematodes [10–13]. In all these cases, the endosymbionts are obligatory mutualists—they are essential for the survival of their host species. Curiously, examples of Wolbachia infections that are facultative and provide a fitness benefit are rare (e.g., [14,15]). One would, however, expect them to be frequent, since these are long-term symbioses, and Wolbachia fitness ultimately depends on the host fitness.
The model organism Drosophila melanogaster can also be infected with Wolbachia. In fact, they are detected in a large proportion of flies of natural populations and laboratory stocks [16–18]. Interestingly the presence of wMel, the Wolbachia strain associated with D. melanogaster, does not seem to cause a strong phenotype. wMelPop, a Wolbachia variant from a laboratory stock, does causes tissue degeneration and significantly shortens the lifespan of its carriers . The appearance of this strain may be an artifact of conditions in which laboratory stocks are kept, because no wMel variant from natural populations with these characteristics has been discovered. Wolbachia also rescues the sterility of Sex-lethal hypomorphic mutants . However, it is not known how this translates to the interaction of Wolbachia with wild-type flies. How natural variants of Wolbachia affect wild-type D. melanogaster has been extensively addressed. wMel only induces a weak and transient CI phenotype in D. melanogaster [21–23], although some Wolbachia strains induce strong CI in the closely related D. simulans . This low CI cannot explain how Wolbachia spreads and is maintained in wild-type populations, especially considering that, in the wild, infection is not vertically transmitted with 100% fidelity . A strong hypothesis to explain Wolbachia presence in natural populations is that Wolbachia gives a fitness benefit to D. melanogaster [26,27]. Several studies have either been unable to find differences in fitness parameters or found only slight beneficial or detrimental effects of Wolbachia infection [25,27–31]. Moreover, even when effects were observed, they were dependent on the Wolbachia variant or the fly's genetic background. A clear strong beneficial effect of Wolbachia infection in D. melanogaster has still not been shown, and it remains a puzzle why these bacteria are so prevalent in natural populations.
D. melanogaster is a valuable tool in the study of resistance to pathogens, with many components of innate immunity signaling pathways conserved between Drosophila and mammals . The epitome of its utility was the discovery of the involvement of Toll-like receptors in innate immunity. Toll was first discovered to be important in the resistance of Drosophila to fungi , later Toll-like receptors were shown to have fundamental functions in mammalian innate immunity . Moreover, Toll-like receptors are important in the activation and modulation of mammalian adaptive immunity.
The responses of Drosophila to systemic infection by fungi and bacteria are increasingly well known . Although less extensively, Drosophila has also been used as a model system to study resistance to viruses. Recent research has shown conservation between flies and mammals in their immune response to viruses. Mutations in hopscotch, the gene that encodes the kinase of the JAK-STAT pathway, reduce resistance to Drosophila C virus (DCV) infection and increases viral titers . Interestingly, in mammals, JAK-STAT pathways are involved in cytokine signaling, including anti-viral type I interferon . Work in Drosophila has also been important in showing that RNA interference is involved in anti-viral resistance in animals. Flies mutant in genes that encode components of this pathway, Dicer-2, Argonaute-2, and r2d2, are more sensitive to infection by several RNA viruses and have higher titers of viruses than the wild type [38–41].
To identify new genes involved in Drosophila resistance to viruses, we initiated a screen for DCV-sensitive flies. In doing so, we found that flies infected with intracellular bacteria were much more resistance to DCV infection than those that were uninfected. We identified these bacteria as Wolbachia and show that DCV titers are much lower in Wolbachia-infected flies. Moreover, resistance to infection extends to two other RNA viruses but not to a DNA virus. These results identify a new major factor involved in Drosophila resistance to RNA viruses, and the first strong beneficial effect associated with Wolbachia infection in D. melanogaster.
Tetracycline Treatment Reduces Resistance to DCV
In order to identify new genes involved in D. melanogaster resistance to viruses, we are conducting a genetic screen for virus-sensitive mutants (LT, AF, MA, unpublished data). We have generated a collection of mutant lines by P-element insertional mutagenesis using the set of w1118 iso isogenic lines described in Ryder et al., 2004 . We chose an isogenic background to minimize variability in the response to viral infection and, for the same reason, cleaned the initial set of lines of potential chronic viral infections using the protocol described in Brun and Plus, 1978 . We test the resistance of each insertion line to DCV infection. DCV is a small, non-enveloped virus with a single-stranded, positive-sense RNA genome that belongs to the Dicistroviridae family, an insect specific family of viruses very similar to picornaviruses . This virus is a natural pathogen of D. melanogaster, it is sequenced and relatively well characterized, and its infection has an easily scored lethal phenotype [43,44].
In the initial screen, we assayed adult survival after intra-thoracic DCV injection and realized that, unexpectedly, the control w1118 iso line was much more sensitive to DCV than most of the tested P-element insertion lines (Figure 1 and unpublished data). When injected with a dose of 500 times the median tissue culture infective dose (TCID50), all the w1118 iso males died within 12 d, whereas very little death was observed in the males of the P-element insertion lines (Figure 1A). Moreover, a large proportion of males of the P-element insertion line survived until 21 d after infection. Preliminary analysis showed that the P-element was not responsible for virus resistance (unpublished data). We then tested the hypothesis that a previous treatment of the w1118 iso line with tetracycline could have rendered it more sensitive to DCV. We raised flies of a P-element insertion line, VF-0058–3, on tetracycline-containing medium or control medium and compared the adults' resistance to DCV infection (Figure 1A). The tetracycline treatment made the flies die much faster upon DCV infection, with a sensitivity similar to that of w1118 iso line. This result strongly suggested that a tetracycline-sensitive bacteria, associated with the resistant stocks, conferred resistance to the viral infection. We discarded the possibility that the effect was an artifact of the tetracycline per se because raising w1118 iso flies on medium with tetracycline did not make them more sensitive to DCV (Figure 1B). We also treated the VF-0058–3 and VF-0097–3 P-element insertion lines with tetracycline for two generations and moved them back to normal medium for at least five generations in order to negate any side effects of tetracycline itself (these stocks will be referred as VF-0058–3t and VF-0097–3t). We then repeated the assay, comparing resistance to DCV of these treated stocks to the non-treated stocks (Figure 1C). The tetracycline treatment makes the P-element insertion lines stably more sensitive to DCV than non-treated lines and equally sensitive to DCV as the w1118 iso line. A similar result was obtained when females of these lines were injected with DCV, with the difference that females are less sensitive to DCV than males (unpublished data). Importantly, in the timeframe of this assay, the survival of tetracycline-treated and non-treated stocks do not differ when only injected with buffer (Figure 1D). In summary, these results show that tetracycline-sensitive bacteria, not easily acquired from the laboratory environment, confer on D. melanogaster resistance to DCV.
(A, B, and C) Fifty 3–6-d-old males, per sample, were injected with DCV, and their survival was followed daily. (A) Flies w1118 iso, VF-0058–3, and VF-0058–3 raised on tetracycline for one generation were injected with 500 TCID50 DCV. (B) Flies w1118 iso and w1118 iso raised on tetracycline were injected with 50 TCID50 DCV. (C) Flies w1118 iso, VF-0058–3, VF-0058–3t, VF-0097–3, and VF-0097–3t were injected with 500 TCID50 DCV. Each assay was repeated once with males and twice with females with similar results.
(D) Fifty 3–6-d-old males, per sample, of VF-0058–3 and VF-0058–3t lines were injected with 50 mM Tris-HCl, pH 7.5, kept at 18°C and their survival was followed daily.
(E) Extracts of VF-0058–3 and VF-0058–3t flies 3 and 6 d after injection with 500 TCID50 DCV or not injected were probed in a Western blot with anti-DCV. Anti-tubulin was used as a loading control.
(F) Titration, in cell culture, of DCV levels per fly of VF-0058–3 and VF-0058–3t flies 3 and 6 d after injection with 500 TCID50 DCV. Squares are replicates (four per sample), lines are geometric means of replicates. Virus titres in VF-0058–3 and VF-0058–3t are significantly different on both days post-infection (Mann-Whitney test, p = 0.0287 for both comparisons).
The increased resistance to DCV could be due to increased resistance to the damage caused by the viral infection or decreased viral proliferation. To test this, we probed by Western blot the levels of viral proteins in VF-0058–3 and VF-0058–3t adult flies after infection with DCV (Figure 1E). While viral proteins were not detectable on extracts of DCV-infected VF-0058–3 flies, they were clearly detectable on extracts of DCV-infected VF-0058–3t flies, and their levels increased from 3 to 6 d post-infection. We extended this analysis by quantifying, in cell culture, the viral titer in these flies after DCV infection (Figure 1F). DCV is detected after infection in flies from both stocks and slightly increases from 3 to 6 d post-infection. However, DCV levels are considerable higher, by approximately 10,000 times, in VF-0058–3t flies. These experiments show that the bacteria that confer resistance to DCV infection interfere with the virus proliferation.
Identification of the Viral Resistance–Inducing Bacteria
The fact that the tetracycline treatment permanently renders the flies sensitive to DCV shows that the bacteria are not easily acquired from the laboratory environment. To test if the resistance could be horizontally acquired, we raised together the progeny of w1118 iso females (without resistant-inducing bacteria) with the progeny of VF-0058–3 females (with resistant-inducing bacteria) and then assayed the levels of viral proteins in infected flies (Figure 2A). The progeny of these females can be distinguished by their eye color, due to the presence of a functional white gene, in the RS3 transposon, only in the progeny of VF-0058–3 flies. w1118 iso flies do not acquire the resistance to DCV when raised mixed with the progeny of VF-0058–3 flies. Therefore, the bacteria that confer resistance to DCV are not acquired horizontally. We then tested if the resistance was vertically transmitted by crossing males and females from VF-0058–3 and VF-0058–3t stocks in all four possible combinations and assaying the survival of the adult progeny after DCV infection (Figure 2B). The results clearly show that the determinant factor of the progeny resistance is the mother's resistance; therefore, the bacteria in question are maternally transmitted, which strongly suggests they are intracellular. However, the bacteria could, in theory, be only transmitted by the mother but not be intracellular (e.g., they could be deposited on the egg surface). This did not seem to be the case, because flies that were raised from VF-0058–3 surface-sterilized embryos did not become more sensitive to DCV (Figure 2C). Moreover, we could visualize the presence of intracellular bacteria by DNA staining in embryos from VF-0058–3 and VF-0097–3 stocks but not from VF-0058–3t, VF-0097–3t, or w1118 iso stocks (Figure 2D). We can therefore conclude that the viral resistance is mediated through maternally transmitted intracellular bacteria.
(A) Extracts of flies 6 d after injection with 500 TCID50 DCV were probed in a Western blot with anti-DCV. Anti-tubulin was used as a loading control. Flies used were: w1118 iso ; w1118 iso raised with VF-0058–3; VF-0058–3; and progeny of VF-0058–3 raised with w1118 iso.
(B and C) Fifty 3–6-d-old males, per sample, were injected with 500 TCID50 DCV, and their survival followed daily. (B) Males and females from resistant (VF-0058–3) and sensitive (VF-0058–3t) stocks were crossed in the four possible combinations, and their progeny were tested for DCV resistance. The assay was repeated with females with similar results. (C) VF-0058–3 embryos were surface sterilized with bleach, raised to adults, and their resistance to DCV compared with non-treated VF-0058–3 and w1118 iso flies. The assay was repeated with females with similar results.
(D) DNA staining, with propidium iodide, of 0–2 h embryos of VF-0058–3, VF-0058–3t, w1118 iso, VF-0097–3, and VF-0097–3t. Extranuclear DNA staining corresponds to bacteria. Scale bar, 10μm.
(E) PCR amplification with wsp and wspB primers on DNA extracts of VF-0058–3t, VF-0058–3, VF-0097–3t, and VF-0097–3 embryos. PCR amplification with mt 12S rRNA primers was done as a DNA extraction control.
(F) PCR amplification with primers specific for Spiroplasma 16S rRNA gene on DNA extracts of RED-67, VF-0058–3, wt 1, wt 2, wt 3, wt 4, wt 5, and wt 6 adults. PCR amplification with mt 12S rRNA primers was done as a DNA extraction control.
To identify the intracellular bacteria in question, we extracted DNA from surface-sterilized embryos of resistant-to-DCV flies (VF-0058–3) and performed PCR amplification using prokaryotic 16S rRNA universal primers. We analysed the product of this amplification by cloning it and sequencing over 100 independent clones. All the 104 sequences of inserts in the cloning plasmid we obtained were at least 99.5% identical to the sequence of a fragment of the 16S rRNA gene of Wolbachia (GenBank accession number EU096232; http://www.ncbi.nlm.nih.gov/Genbank/). Therefore, these embryos, which carry the resistance to DCV inducing bacteria, are most probably only infected with Wolbachia. To verify the presence of Wolbachia, we performed PCR amplification using primers for the Wolbachia specific genes wsp and wspB [45,46] (Figure 2E). Wolbachia is present in VF-0058–3 and VF-0097–3 and absent from the VF-0058–3t and VF-0097–3t embryos' extracts. The sequence of the wsp-specific primers' PCR amplification product from the VF-0058–3 flies is identical to the wsp sequence of wMel (GenBank accession number DQ235407), the only Wolbachia strain known to infect D. melanogaster. In a recent survey in 35 different Drosophila species, that screened over 4,500 individuals, only two kind of heritable endosymbiotic bacteria were found: Wolbachia and Spiroplasma . We specifically tested for the presence of Spiroplasma in the VF-0058–3 line (and the wt-1 to -6 lines used in Figure 3B) using primers specific for the 16S rRNA gene of Spiroplasma . We detect Spiroplasma in a positive control, RED-67 , but not in any of the other tested lines. Therefore it is not Spiroplasma that confers resistance to viruses. This result and the sequencing results strongly suggest that the maternally inherited intracellular bacteria that confer resistance to DCV are Wolbachia.
(A) Two independent sets of isofemales lines were established from VF-0058–3 flies raised on a sub-optimal dose of tetracycline (lines 1–10 and 11–33). The presence of Wolbachia in these lines was tested by PCR amplification using wsp primers on DNA extracts of adult flies, PCR with mt 12S rRNA primers was done as a control. Three–six-d-old males of each line were injected with 500 TCID50 DCV, collected 6 d later, and DCV levels analysed by Western blot with anti-DCV. Anti-tubulin was used as a loading control.
(B) Six wild-type lines infected with Wolbachia (lines 1–6) and six wild-type lines not infected (lines 7–12) were identified by PCR amplification with wsp primers (more information on wild-type lines identity can be found in Materials and Methods). Each stock was treated with tetracycline for two generations and then transferred to tetracycline-free food for at least two generations. Treated and non-treated stocks were re-tested for presence of Wolbachia by PCR amplification with wsp primers. Seven–twelve PCR amplifications were all negative, not shown. Three–six-d-old males of each stock (lines 1–12, tetracycline treated and non-treated) were injected with 500 TCID50 DCV, collected 6 d later, and DCV levels analysed by Western blot with anti-DCV. Anti-tubulin was used as a loading control.
These results show that resistance to DCV is associated with the presence of Wolbachia, however it could be possible that other cryptic intracellular bacteria were responsible for the virus resistance, and Wolbachia would merely be present in these flies by chance. Wolbachia cannot be cultured and therefore we cannot infect a sensitive stock with a pure cultured isolate and verify acquired resistance to DCV. Wolbachia can be artificially transferred from an infected host to a new host. However, if we did transfer Wolbachia from infected flies to non-infected flies and show concomitant transfer of resistance to DCV, we could not discard the possibility that we were also transferring the hypothetical cryptic bacteria. We addressed this problem by treating the Wolbachia-infected stock VF-0058–3 with a suboptimal dose of tetracycline for one generation and then establishing isofemale lines from the progeny. We expected to obtain lines that kept the Wolbachia infection and other lines that lost it. The segregation of Wolbachia should be independent of the segregation of any hypothetical other bacteria. From two independent sets, one set of ten lines and another set of 23 lines, we established, in total, three lines that conserved Wolbachia infection and 30 lines that lost it. We then tested these lines for resistance to viruses (Figure 3A). Wolbachia presence and viral resistance fully segregate with each other; the probability that the presence of Wolbachia and resistance to DCV are independent is very low (Fisher's exact test, p = 0.0002). These data strongly indicate that it is Wolbachia infection that induces DCV resistance.
To corroborate that it is Wolbachia infection that protects D. melanogaster from DCV, we analyzed this interaction in other independent fly stocks. We screened, by PCR, for Wolbachia presence in a collection of wild-type stocks kept in our laboratory and we found six infected lines. After establishing tetracycline-treated stocks derived from these lines, we compared, by Western blots, their resistance to DCV with the original lines (Figure 3B). In all the six cases, the loss of Wolbachia makes the flies more sensitive to DCV. The same procedure was applied to six stocks that did not carry Wolbachia initially (Figure 3B). There is much heterogeneity in the levels of DCV proteins 6 d after infection in these stocks (e.g., line 7 is very resistant to DCV infection), but, importantly, there is no increase in the levels of DCV proteins in the tetracycline treated stocks. These results show that increase sensitive to DCV after tetracycline treatment is always associated with an initial Wolbachia infection. From this set of data, plus the same result of increased sensitivity with tetracycline treatment of VF-0058–3, we can state that the probability that initial Wolbachia infection and increased sensitivity upon tetracycline treatment are independent is very low (Fisher's exact test, p = 0.0006). In conclusion, we can confidently state that it is Wolbachia that protects D. melanogaster from DCV infection.
Wolbachia Effect on Other Viral Infections
Wolbachia and DCV are commonly found in D. melanogaster natural populations and laboratory stocks; their interaction could be very specific. We investigated if Wolbachia protection extends to infections by two other RNA viruses and a DNA virus.
Nora virus is a recently described common natural pathogen of D. melanogaster . Similar to DCV, Nora virus is a small, non-enveloped virus with a single-stranded, positive-sense RNA genome. It is similar to picornaviruses and dicistroviruses, but it has a unique genome organization. Using reverse-transcription PCR (RT-PCR) with Nora virus–specific primers, we found that the VF-0058–3 and VF-0058–3t stocks are not infected with it while another laboratory stock, Oregon R, is (Figure 4A). An extract of Oregon R adult flies was injected into VF-0058–3 and VF-0058–3t adult flies, and the levels of Nora virus replication were accessed, by semi-quantitative RT-PCR, after 3 d (Figure 4A). We observed, in four independent repeats, that Nora virus levels are lower in Wolbachia-infected flies; Wolbachia infection also protects Drosophila from Nora virus infection.
(A) RT-PCR was done with Nora virus primers on RNA of VF-0058–3, VF-0058–3t, and Oregon R flies (left). PCR with RpL32 was done as control. Three–six-d-old males of VF-0058–3 and VF-0058–3t lines were injected with a virus extract of Oregon R flies and collected 3 d later. RNA was extracted and RT-PCR done with primers for Nora virus and RpL32. The same number of PCR cycles was done for both samples. The assay was repeated three more times, from infection of flies with virus extract, with similar results.
(B) Fifty 3–6-d-old males, per sample, of VF-0058–3 and VF-0058–3t lines were injected with 50 TCID50 FHV, and their survival was followed daily. The assay was repeated twice with males and once with females with similar results.
(C) Extracts of VF-0058–3 and VF-0058–3t flies 3, 6, and 9 d after injection with 50 TCID50 FHV or not injected were probed in a Western blot with anti-FHV. Anti-tubulin was used as a loading control.
(D) Titration, in cell culture, of FHV levels per fly of VF-0058–3 and VF-0058–3t flies 6 d after injection with 50 TCID50 FHV. Squares are replicates (10 per sample), lines are geometric means of replicates. Virus titres in VF-0058–3 and VF-0058–3t are not significantly different (Mann-Whitney test, p = 0.05764).
(E) Fifty 3–6-d-old males, per sample, of VF-0058–3 and VF-0058–3t lines were injected with 1,000 TCID50 IIV-6 or buffer, and their survival followed. The assay was repeated once with males and the IIV-6 injected flies survival curves were also repeated with females, with similar results.
(F) Iridescent-infected male 20 d after injection with 1000 TCID50 IIV-6 (right) and not infected same age male (left) are shown.
(G) Titration, in cell culture, of IIV-6 levels per fly of VF-0058–3 and VF-0058–3t flies 10 days after injection with 1000 TCID50 IIV-6. Squares are replicates (10 per sample), lines are geometric means of replicates. Virus titres in VF-0058–3 and VF-0058–3t are not significantly different (Mann-Whitney test, p = 0.08118).
Flock House virus (FHV) belongs to the Nodaviridae family of insect viruses. These are small viruses containing two single-stranded, positive-sense genomic RNAs . FHV was isolated from a coleopteran  and is not a natural pathogen of D. melanogaster. However, it can be cultured in D. melanogaster cells and proliferates and causes death in adult flies when injected [38,50]. We injected FHV in VF-0058–3 and VF-0058–3t adult flies and followed their survival (Figure 4B). Wolbachia-infected flies were much more resistant to FHV infection; with an infection dose of 50 TCID50, all VF-0058–3t flies die by day 13, while only 40% of VF-0058–3 flies die by day 21. We can detect, by Western blot, increase in FHV proteins with time in both infected stocks (Figure 4C). Surprisingly, we only detected a slight increase in FHV proteins in the infected VF-0058–3t flies, compared with the infected VF-0058–3 flies. We confirmed this result by determining, in cell culture, the viral titer per infected fly, 6 d post-infection (Figure 4D). We find, on average, only 1.8-fold more FHV in Wolbachia-free flies. and the difference is not statistically significant (Mann-Whitney test, p = 0.05764). We can conclude that Wolbachia presence also increases the resistance of D. melanogaster to FHV infection although it does not or only slightly affects FHV levels.
Finally, we wanted to test the effect of Wolbachia on a DNA virus infection; however, there is no known DNA virus that is a natural pathogen of D. melanogaster. Insect Iridescent Virus 6 (IIV-6) (also named Chilo iridescent virus (CIV)) is a large virus with a double-stranded DNA genome from the Iridoviridae family . It was first isolated from a lepidopteran but can infect a large number of different insects and cultured insect cells [53,54]. IIV-6 can infect and replicate in D. melanogaster cells  and cause adult flies death upon injection (Peter Christian, personal communication). We confirmed that IIV-6 infection causes premature death in adult flies, approximately halving their lifespan when 1,000 TCID50 are injected per fly (Figure 4E). Importantly we can show that IIV-6 replicates in adult D. melanogaster. Infected flies become iridescent as they accumulate virions, a characteristic of iridoviruses due to their paracrystalline packing (Figure 4F). Moreover, IIV-6 titer per fly after 10 d of infection, determined in cell culture, was approximately 109 TCID50, when a dose of 103 TCID50 was injected (Figure 4G). Contrary to the results obtained with DCV and FHV infection, Wolbachia-infected flies actually died faster than Wolbachia-free flies when infected with IIV-6 (Figure 4E). This probably represents just a cumulative effect of the deleterious effects of Wolbachia and IIV-6 infection. In fact, Wolbachia infection has a long-term deleterious effect that results in a shorter lifespan in the absence of viral infection (Figure 4E). In accordance with this interpretation, the average IIV-6 titer, 10 d after injection, is only 1.8-fold higher in Wolbachia-infected flies compared with Wolbachia-free flies, and not significantly different (Mann-Whitney test, p=0.08118) (Figure 4G). In conclusion, Wolbachia presence does not protect D. melanogaster from IIV-6 infection.
We have shown that Wolbachia infection in D. melanogaster induces resistance to DCV infection. Several lines of evidence lead to this conclusion. The resistance to DCV was maternally transmitted and sensitive to tetracycline, as is Wolbachia; in embryos infected with bacteria inducing resistance to DCV, we can only detect the presence of Wolbachia; all tested D. melanogaster lines that carried Wolbachia became more sensitive to DCV after tetracycline treatment; lines that did not carry Wolbachia did not become more sensitive to DCV after tetracycline treatment. Finally, when transmission to the next generation was imperfect, due to treatment of larvae with a low dose of tetracycline, Wolbachia and resistance to DCV co-segregated. Following Occam's razor principle—Pluralitas non est ponenda sine necessitate. “Plurality should not be posited without necessity.” —the simplest and most plausible hypothesis is that Wolbachia is the causative agent of resistance to DCV.
Infection by Wolbachia considerably increased the lifespan of DCV-infected flies. This is due to a strong reduction in viral titers, as observed by Western blot and titration by cell culture. At 3 d post infection, the DCV titer in Wolbachia-infected flies was 10,000 times less than that in Wolbachia free flies. This difference is larger than that reported between the wild type and mutants in the anti-viral resistance genes Dcr-2, ago-2, and hop [36,38,40]. Wolbachia is clearly a major factor affecting Drosophila resistance to DCV.
Wolbachia and DCV are common symbionts of D. melanogaster. However, the interaction is not specific to DCV; we found that Wolbachia also induced resistance to two other RNA viruses. In the case of Nora virus, there was also reduction in the viral titer of infected flies. FHV infection, in terms of mortality, was also much less severe in the presence of Wolbachia, to a degree similar to that seen with DCV. But, with this virus, Wolbachia only slightly affected viral titer. The resistance to FHV is most probably an increase in resistance to the damaged caused by the viral infection rather than an ability to inhibit virus proliferation. However, we cannot exclude the possibility that there is strong inhibition of FHV proliferation in certain essential adult tissues or that a small decrease in viral titer is enough to significantly increase the lifespan of infected individuals.
DCV and Nora virus differ from FHV in two ways: they are both natural pathogens of Drosophila and both are picornavirus-like. An endogenous virus and its host could be co-adapted so that a small advantage, in this case provided by the bacteria to the host, would profoundly tilt the equilibrium between virus and host, whereas an exogenous pathogen may be less sensitive to bacterial infection of its host. On the other hand, Wolbachia could interfere with the life cycle of picornavirus-like viruses but not of FHV, a nodavirus. We cannot distinguish between these possibilities with such a small sample of viruses; it would be interesting to extend the analysis to other RNA viruses that infect D. melanogaster (e.g., Sigma (a rhabdovirus) , Drosophila X virus (a birnavirus) , and Drosophila A virus (picornavirus-like) ).
We have also tested the interaction of Wolbachia with a DNA virus, IVV-6. Wolbachia did not protect Drosophila from this virus; it actually decreased the lifespan of infected flies. We think this is due to the cumulative effect of Wolbachia and IIV-6 infection, since, in the genetic background of the flies we were using, Wolbachia had a negative effect on long-term survival. It would be interesting to also extend the analysis to other DNA viruses, however there are no DNA viruses known to infect D. melanogaster. To our knowledge, this is the first report of a DNA virus proliferating in adults of D. melanogaster.
An obvious question is how Wolbachia induces resistance to RNA viruses. The different effect on DCV/Nora virus and FHV raises the possibility that this effect is multifactorial; interfering with virus replication in some cases and increasing resistance of Drosophila to viral infection damage in others. One important question to address is whether the effect is cell-autonomous or systemic. Wolbachia is widespread throughout tissues of the infect host [18,56], so both hypotheses are possible. This could be investigated in tissue culture with Wolbachia-infected cells. If the effects are cell autonomous, one explanation for increased resistance to viruses could just be competition for resources, since both microorganisms occupy the same niche, the host's cytoplasm. For example, Wolbachia is thought to acquire much of its energy from the metabolism of amino acids imported from the host cytoplasm . DCV, on the other hand, is very sensitive to perturbations in host translation . The presence of Wolbachia could reduce the pool of cytoplasmic amino acids to a point that interferes with translation of viral proteins. Another possibility is that Wolbachia infection could trigger cell-autonomous mechanisms of resistance to intracellular pathogens, such as a reduction in cellular metabolism. A further explanation for a cell-autonomous effect would be that Wolbachia has been selected to actively interfere with virus replication in co-infected cells. Wolbachia has a complete type IV secretion system , which many bacteria use for translocation of effector molecules into host cells (e.g., Legionella and Agrobacterium). Genes encoding proteins with ankyrin repeats, involved in protein–protein interactions, are over-represented in the Wolbachia genome [46,58] and are good candidates for mediators of anti-viral resistance.
If the effect is systemic, a strong hypothesis is that Wolbachia could alter the host–immune response, increasing resistance to viral infection. The pre-activation of the host immune system, for example, could allow for a faster response upon viral infection. This would be similar to what happens in a herpesvirus-induced resistance to Listeria in mice, due to the production of cytokines . It was also reported recently that the presence of gut flora slightly increases the resistance of Aedes aegypti to Dengue virus, presumably through activation of the Toll pathway . In tissue culture of D. melanogaster cells, infection with Wolbachia slightly increases the expression of innate immune genes . There is also a report that Wolbachia increases resistance of D. melanogaster to the pathogenic fungus Beauveria bassiana . All these reports support a model of general activation of innate immunity. However it has also been shown that in adult D. simulans and Aedes albopictus Wolbachia does not activate the expression of anti-microbial peptides , in D. simulans, Wolbachia infection does not alter sensitivity to Beauveria and renders the host more sensitive to parasitoid wasps , and in D. melanogaster, Wolbachia presence does not affect Spiroplasma levels . In summary, it is not clear if there is a general activation of innate immunity in adult D. melanogaster infected with Wolbachia that would render them more resistant to other pathogens. It would be interesting to identify immune pathways involved in anti-viral resistance activated by Wolbachia infection. It would also be important to analyze Wolbachia-induced resistant to other microorganisms, including pathogenic bacteria.
A different hypothesis would be that Wolbachia infection actually inhibits some of the immune responses against viral infection and that increases the lifespan of infected D. melanogaster. This may be true if the host response to infection damages the host itself, as in the case of septic shock in mammals. This could explain the increased resistance to FHV infection without a strong effect on viral titers. Finally, a similar hypothesis would be that Wolbachia inhibits, cell-autonomously or systemically, apoptosis induced upon viral infection. Some published data support this hypothesis; FHV induces apoptosis in tissue culture cells , Wolbachia inhibits apoptosis in the germline of Asobara tabida [8,9], and the Wolbachia protein Wsp inhibits apoptosis in human cells .
This new host-microorganism-microorganism interaction adds to the perception that the response of a host to a particular pathogen also depends on its interactions with other microorganisms. Other examples are herpesvirus latency-induced protection to Listeria in mice mentioned above , the suppression of HIV-1 infection by human herpesvirus 6 in human cells , symbiotic bacteria protection against fungi in a shrimp and an aphid [69,70], symbiotic bacteria protection against parasitic wasps in an aphid , and symbiotic bacteria protection against fungal infection in a wasp . As also mentioned above, there is a recent report that gut flora has a protective role against Dengue virus in A. aegypti . However, this is, to our knowledge, the first report where bacteria that confer protection against viruses have been identified.
This interaction has some practical consequences. Researchers working on Drosophila immunity against viruses should take in consideration the presence of Wolbachia in the stocks they are analyzing. On the other hand, researchers working on Wolbachia should consider that any observed effects of Wolbachia could be mediated through effects on viral infections. A practical application of this discovery would be, if possible, to induce resistance to viruses, by infection with Wolbachia, in insects that are beneficial to humans (e.g., honeybee) or transmit arboviruses (e.g., mosquitoes). However, introducing Wolbachia to virus-transmitting vectors could be a double-edged sword. If the interaction Wolbachia-vector-virus were similar to the one seen in this report with DCV, then it would be beneficial because it could decrease the probability of the vector being infected or transmitting the disease. If, however, it were similar to the interaction with FHV, then there would be the risk of having healthier infected vectors with high titers of viruses, therefore increasing disease transmission. This latest possibility should be taken into account in proposed strategies of introducing Wolbachia in vectors of arboviruses [73,74].
Finally, this is, to our knowledge, the first report of a strong beneficial effect of Wolbachia infection in D. melanogaster. The induced resistance to natural viral pathogens may explain the prevalence of Wolbachia in natural populations. It also indicates that the fitness benefit of having Wolbachia is dependent on the viral infection status of the population. This may explain differences in Wolbachia infection frequencies between populations [17,25] and variable fitness effects in different D. melanogaster lines [28,30]. It would be interesting to broaden the analysis to other Wolbachia strains and to other Wolbachia–host combinations. If Wolbachia induces resistance to viruses in other hosts, this would have major implications for our understanding of the very widespread presence of this endosymbionts in arthropods and filarial nematodes.
After the submission of this manuscript, an independent report with similar findings to ours was published . In agreement with our data, Hedges et al. show that the treatment of Wolbachia-infected flies with tetracycline renders them more sensitive to three RNA viruses: DCV, Cricket Paralysis virus, and FHV. Moreover, they also show that the levels of DCV increase in infected Wolbachia-free flies.
Materials and methods
Fly strains and husbandry.
The set of w1118 iso isogenic flies were obtained from the DrosDel collection in our laboratory . These lines were cleaned of viruses similarly to the protocol in Brun and Plus, 1978 . Flies were aged to 30 d at 25 °C and their eggs were collected in agar plates, treated with 50% bleach for 10 min, washed with water, and transferred to fresh vials.
Origins of Wild-Type Laboratory Lines Used in This Work
Stocks were treated with tetracycline (cleaned of Wolbachia infection) by raising them for two generations in ready-mix dried food (Philip Harris) with 0.05 mg/ml of tetracycline hydrochloride (Sigma). Sub-optimal tetracycline treatment was done by raising flies, for one generation, in food with 0.00625 mg/ml of tetracycline hydrochloride. Virgin adult females, emerging from these vials, were collected and individually crossed with males to establish isofemale lines.
Sensitive and resistant-to-viruses flies were raised together by placing in a vial one w1118 iso female, one VF-0058–3 female, and two w1118 iso males. The progeny of the two different females could be distinguished by the eye color, because only the progeny of VF-0058–3 females have a functional white gene. Flies were only collected from vials that had adults of both phenotypes.
Nora virus extract was prepared from a naturally infected Oregon stock present in the laboratory. Thirty adult flies were squashed in 900 μl of 50 mM Tris-HCl, pH 7.5. Extract was then frozen at −80 °C, thawed and twice centrifuged for 10 min at top speed in a tabletop centrifuge, at 4 °C. The supernatant was aliquoted and stored at −80 °C.
IIV-6  was kindly given by Dr. Peter Christian and raised in Schneider Drosophila line 2 (DL2) cells . DL2 cells were kept in Schneider's Drosophila Medium (Invitrogen) supplemented with 10% Fetal Bovine Serum, 2mM L-Glutamine, 100 U/ml penicillin, and 100μg/ml streptomycin (all Invitrogen). Seven days after infection, the cell culture was collected and frozen at −80 °C. The culture was thawed, frozen, and thawed again to disrupt cells and centrifuged twice at 600g for 10 min to remove cell debris. Virus was pelleted by centrifugation at 10,000g for 10 min and re-suspended in water. Virus suspension was placed over a 30% sucrose solution and the virus was pelleted again by centrifugation at 30,000g for 30 min and re-suspended in water. The virus was then pellet by centrifugation at 15,000g for 10 min and re-suspended twice. The final re-suspension was done in 50 mM Tris-HCl, pH 7.5, aliquoted, and stored at −80 °C. All centrifugations and re-suspensions were done at 4 °C.
FHV was kindly provided by Dr. J.-L. Imler  and dilutions of this aliquot were used in this work.
Three-to-six–d-old flies were injected with a Nanoject II injector (Drummond). Viruses were re-suspended or diluted in 50 mM Tris-HCl, pH 7.5, and 69 nl of virus solution was injected, per fly, in the thorax, between the mesopleura and the pteropleura. Flies were injected while anesthetized with CO2. Fifty flies were injected per sample, ten flies were placed per vial, and vials were changed twice a week. DCV injected flies were kept at 18 °C. Nora virus–, FHV- and IIV-6–injected flies were kept at 25 °C. Flies were counted daily for all survival curves except for VF-0058–3 and VF-0058–3t injected with buffer, as shown in Figure 4E, which were counted at least twice a week.
Five flies were pooled per sample. Flies were squashed in 50 mM Tris-HCl, pH 7.5, frozen, thawed, and centrifuged for 10 min at 20,000g and supernatant was collected (DCV and FHV). For IIV-6, centrifugation was done twice at 600g and the supernatant passed through a 0.45-μm filter before the assay. Viruses titers were determined in cell culture and calculated by the Reed and Muench end-point calculation method . DL2 cells in 96-well plates were infected with the serial dilutions of virus suspensions. DCV and FHV infection was scored by the presence of cell death, IIV-6 was scored by non-proliferation of cells and presence of very large cells. Extracts of non-infected VF-0058–3 or VF-0058–3t flies did not cause any cytopathic effect in tissue culture cells.
Five to eight males were pooled per sample. Rabbit polyclonal antibodies raised against purified DCV was kindly given by Dr. Peter Christian. Rabbit polyclonal antibodies raised against FHV capsids was kindly given by Dr. Jean-Luc Imler . Specificity of antibodies was verified by lack of signal on Western blot lanes of non-infected flies (Figures 1E and 4C). E7 mouse monoclonal anti-β-tubulin was acquired from Developmental Studies Hybridoma Bank .
Propidium iodide staining.
Embryos 0–2-h-old were collected, treated with 50% commercial bleach for 10 min, fixed for 30 min in 4% formaldehyde, 50% heptane, the vitelline membranes were removed by vortexing the embryos in 50% heptane, 50% methanol, then embryos were washed briefly in methanol and for 10 min in 50% methanol, 50% PBS and finally placed in PBS 0.1% tween-20. Embryos were treated with RNAse H 0.25 μg/μl for 30 min at 37 °C, washed in PBS 0.1% tween-20, stained with PBS 0.1% tween-20 and 1 μg/ml propidium iodide (Sigma) for 30 min, washed in PBS 0.1% tween-20 and mounted in Vectorshield. Images were taken in a confocal microscope.
PCR, sequence analysis, and RT-PCR.
A fragment of bacterial 16S rRNA gene was amplified from DNA of Drosophila embryos surface sterilized by treatment with 50% commercial bleach for 10 min. DNA was extracted using Wizard Genomic DNA purification kit (Promega). Primers used were 27f (5′-GAGAGTTTGATCCTGGCTCAG-3′) and 1495r (5′-CTACGGCTACCTTGTTACGA - 3′). The PCR program was: 94 °C for 4 min; 25 cycles of 94 °C for 30 s, 58 °C for 1 min, and 72 °C for 2 min; 72 °C for 10 min. The PCR product was ligated into pCR 2.1 TOPO vector (Invitrogen) and transformed into DH5α cells. Nineteen plasmid DNA preparations and 96 bacteria cultures were sent for sequencing. Three sequencing reactions failed and 8 clones did not carry an insertion in the cloning plasmid. From all the other 104 sequences, we selected a sequence of at least 600 bp with good quality and aligned it with a fragment of the 16S rRNA gene of Wolbachia (GenBank accession number EU096232) using Clustal W 2 .
PCR amplification of Wolbachia-specific genes was done either on the DNA extracts of embryos as described above (Figure 2E) or on DNA extracts of adult flies (Figure 3). Adult flies were squashed in 25 mM NaCl, 10 mM Tris-HCl pH=8.0, 1 mM EDTA, 200 μg/ml proteinase K and incubated for 30 min at 37 °C. Proteinase K was inactivated at 95 °C for 5 min. The supernatant was directly used for PCR amplification. wsp primers were wsp 81F (5′-TGGTCCAATAAGTGATGAAGAAAC-3′) and wsp 691R (5′-AAAAATTAAACGCTACTCCA-3′) . wspB primers were wspB-F (5′-TTTGCAAGTGAAACAGAAGG-3′) and wspB-R (5′-GCTTTGCTGGCAAAATGG-3′) . As a positive control for cytoplasmic DNA extraction we used the primers for mitochondrial 12S rRNA, 12SAI (5′-AAACTAGGATTAGATACCCTATTAT-3′) and 12SBI (5′-AAGAGCGACGGGCGATGTGT-3′) . The PCR program used was: 94 °C for 4 min; 30 cycles of 94 °C for 1 min, 55 °C for 1 min, and 72 °C for 1 min; 72 °C for 10 min. The wsp primers amplification product from VF-0058–3 embryos was purified, as described above, and sequenced. The sequence obtained was identical to a fragment of the wsp sequence of wMel (GenBank accession number DQ235407).
PCR amplification with primers specific for Spiroplasma 16S rRNA gene was done on DNA extracts of adult flies. Primers used were SpoulF (5′-GCTTAACTCCAGTTCGCC-3′) and SpoulR (5′-CCTGTCTCAATGTTAACCTC-3′) . The PCR program was: 94 °C for 4 min; 30 cycles of 94 °C for 30 s, 55 °C for 1 min, and 72 °C for 1 min; 72 °C for 10 min. As a positive control for cytoplasmic DNA extraction, we used the primers for mitochondrial 12S rRNA, as described before.
Nora virus presence in VF-0058–3, VF-0058–3t, and Oregon R stocks was analyzed by RT-PCR. RNA of 100 flies, per sample, was extracted using Trizol (Invitrogen). cDNA was synthesized from 5 μg of total RNA with Superscript III (Invitrogen), using random hexamers primers, at 50 °C. The Nora primers used for PCR were Nora-F (5′-TTAAGGTGTTAGAGAACAGC-3′) and Nora-R (5′-CGTAAACACCAACTTACTTC-3′) . RpL32 primers, used as a positive control for RNA extraction, were RpL32-F (5′-TCCTAC CAGCTTCAAGATGAC-3′) and RpL32-R (5′-CACGTTGTGCACCAGGAACT-3′). The PCR program used was as follow: 94 °C for 4 min; 10 cycles of 94 °C for 30 s, 60 °C minus 0.6 °C per cycle for 1 min and 72 °C for 1 min; 20 cycles of 94 °C for 30 s, 54 °C for 1 min, and 72 °C for 1 min; 72 °C for 10 min. The PCR amplification fragment obtained with the Nora primers was purified, as described above, and sequenced. The sequence was 98% identical to a fragment of Nora virus genome sequence (GenBank accession number DQ321720). For semi-quantitative analysis of Nora virus in infected flies, the procedure was as above except that 25 flies were used per sample and the PCR amplification for each sample was done with a total of 20, 25, and 30 cycles.
We would like to thank Peter Christian for stocks of DCV and IIV-6, antibodies against DCV, invaluable help in the setup of several viruses-related protocols, and discussions. We would like to thank Jean-Luc Imler for kindly providing the FHV stock and antibodies against FHV. We would like to thank Gregory Hurst for kindly providing the RED-67 strain. We would also like to thank John Roote for help related with laboratory fly stocks.
LT conceived and designed the experiments. LT and AF performed the experiments. LT analyzed the data. LT and MA wrote the paper. MA also provided support and discussions.
- 1. Werren JH, Windsor D, Guo LR (1995) Distribution of Wolbachia among neotropical arthropods. Proc R Soc Lond B Bio 262: 197–204.JH WerrenD. WindsorLR Guo1995Distribution of Wolbachia among neotropical arthropods.Proc R Soc Lond B Bio262197204
- 2. Jeyaprakash A, Hoy MA (2000) Long PCR improves Wolbachia DNA amplification: wsp sequences found in 76% of sixty-three arthropod species. Insect Mol Biol 9: 393–405.A. JeyaprakashMA Hoy2000Long PCR improves Wolbachia DNA amplification: wsp sequences found in 76% of sixty-three arthropod species.Insect Mol Biol9393405
- 3. Hilgenboecker K, Hammerstein P, Schlattmann P, Telschow A, Werren JH (2008) How many species are infected with Wolbachia? - a statistical analysis of current data. FEMS Microbiol Lett 281: 215–220.K. HilgenboeckerP. HammersteinP. SchlattmannA. TelschowJH Werren2008How many species are infected with Wolbachia? - a statistical analysis of current data.FEMS Microbiol Lett281215220
- 4. O'Neill SL, Giordano R, Colbert AM, Karr TL, Robertson HM (1992) 16S rRNA phylogenetic analysis of the bacterial endosymbionts associated with cytoplasmic incompatibility in insects. Proc Natl Acad Sci U S A 89: 2699–2702.SL O'NeillR. GiordanoAM ColbertTL KarrHM Robertson199216S rRNA phylogenetic analysis of the bacterial endosymbionts associated with cytoplasmic incompatibility in insects.Proc Natl Acad Sci U S A8926992702
- 5. Hertig M, Wolbach S (1924) Studies on Rickettsia-Like Micro-Organisms in Insects. J Med Res 44: 329–374.M. HertigS. Wolbach1924Studies on Rickettsia-Like Micro-Organisms in Insects.J Med Res44329374
- 6. Yen JH, Barr AR (1973) The etiological agent of cytoplasmic incompatibility in Culex pipiens. J Invertebr Pathol 22: 242–250.JH YenAR Barr1973The etiological agent of cytoplasmic incompatibility in Culex pipiens.J Invertebr Pathol22242250
- 7. Stouthamer R, Breeuwer JA, Hurst GD (1999) Wolbachia pipientis: microbial manipulator of arthropod reproduction. Annu Rev Microbiol 53: 71–102.R. StouthamerJA BreeuwerGD Hurst1999Wolbachia pipientis: microbial manipulator of arthropod reproduction.Annu Rev Microbiol5371102
- 8. Dedeine F, Vavre F, Fleury F, Loppin B, Hochberg ME, et al. (2001) Removing symbiotic Wolbachia bacteria specifically inhibits oogenesis in a parasitic wasp. Proc Natl Acad Sci U S A 98: 6247–6252.F. DedeineF. VavreF. FleuryB. LoppinME Hochberg2001Removing symbiotic Wolbachia bacteria specifically inhibits oogenesis in a parasitic wasp.Proc Natl Acad Sci U S A9862476252
- 9. Pannebakker BA, Loppin B, Elemans CP, Humblot L, Vavre F (2007) Parasitic inhibition of cell death facilitates symbiosis. Proc Natl Acad Sci U S A 104: 213–215.BA PannebakkerB. LoppinCP ElemansL. HumblotF. Vavre2007Parasitic inhibition of cell death facilitates symbiosis.Proc Natl Acad Sci U S A104213215
- 10. Hoerauf A, Volkmann L, Hamelmann C, Adjei O, Autenrieth IB, et al. (2000) Endosymbiotic bacteria in worms as targets for a novel chemotherapy in filariasis. Lancet 355: 1242–1243.A. HoeraufL. VolkmannC. HamelmannO. AdjeiIB Autenrieth2000Endosymbiotic bacteria in worms as targets for a novel chemotherapy in filariasis.Lancet35512421243
- 11. Hoerauf A, Nissen-Pähle K, Schmetz C, Henkle-Dührsen K, Blaxter ML, et al. (1999) Tetracycline therapy targets intracellular bacteria in the filarial nematode Litomosoides sigmodontis and results in filarial infertility. J Clin Invest 103: 11–18.A. HoeraufK. Nissen-PähleC. SchmetzK. Henkle-DührsenML Blaxter1999Tetracycline therapy targets intracellular bacteria in the filarial nematode Litomosoides sigmodontis and results in filarial infertility.J Clin Invest1031118
- 12. Casiraghi M, McCall JW, Simoncini L, Kramer LH, Sacchi L, et al. (2002) Tetracycline treatment and sex-ratio distortion: a role for Wolbachia in the moulting of filarial nematodes. Int J Parasitol 32: 1457–1468.M. CasiraghiJW McCallL. SimonciniLH KramerL. Sacchi2002Tetracycline treatment and sex-ratio distortion: a role for Wolbachia in the moulting of filarial nematodes.Int J Parasitol3214571468
- 13. Bandi C, McCall JW, Genchi C, Corona S, Venco L, et al. (1999) Effects of tetracycline on the filarial worms Brugia pahangi and Dirofilaria immitis and their bacterial endosymbionts Wolbachia. Int J Parasitol 29: 357–364.C. BandiJW McCallC. GenchiS. CoronaL. Venco1999Effects of tetracycline on the filarial worms Brugia pahangi and Dirofilaria immitis and their bacterial endosymbionts Wolbachia.Int J Parasitol29357364
- 14. Vavre F, Girin C, Boulétreau M (1999) Phylogenetic status of a fecundity-enhancing Wolbachia that does not induce thelytoky in Trichogramma. Insect Mol Biol 8: 67–72.F. VavreC. GirinM. Boulétreau1999Phylogenetic status of a fecundity-enhancing Wolbachia that does not induce thelytoky in Trichogramma.Insect Mol Biol86772
- 15. Dobson SL, Marsland EJ, Rattanadechakul W (2002) Mutualistic Wolbachia infection in Aedes albopictus: accelerating cytoplasmic drive. Genetics 160: 1087–1094.SL DobsonEJ MarslandW. Rattanadechakul2002Mutualistic Wolbachia infection in Aedes albopictus: accelerating cytoplasmic drive.Genetics16010871094
- 16. Solignac M, Vautrin D, Rousset F, Rousset F (1994) Widespread occurrence of the proteobacteria Wolbachia and partial cytoplasmic incompatibility in Drosophila Melanogaster. Cr Acad Sci Iii-Vie 317: 461–470.M. SolignacD. VautrinF. RoussetF. Rousset1994Widespread occurrence of the proteobacteria Wolbachia and partial cytoplasmic incompatibility in Drosophila Melanogaster.Cr Acad Sci Iii-Vie317461470
- 17. Hoffmann AA, Clancy DJ, Merton E (1994) Cytoplasmic incompatibility in Australian populations of Drosophila melanogaster. Genetics 136: 993–999.AA HoffmannDJ ClancyE. Merton1994Cytoplasmic incompatibility in Australian populations of Drosophila melanogaster.Genetics136993999
- 18. Clark ME, Anderson CL, Cande J, Karr TL (2005) Widespread prevalence of wolbachia in laboratory stocks and the implications for Drosophila research. Genetics 170: 1667–1675.ME ClarkCL AndersonJ. CandeTL Karr2005Widespread prevalence of wolbachia in laboratory stocks and the implications for Drosophila research.Genetics17016671675
- 19. Min KT, Benzer S (1997) Wolbachia, normally a symbiont of Drosophila, can be virulent, causing degeneration and early death. Proc Natl Acad Sci U S A 94: 10792–10796.KT MinS. Benzer1997Wolbachia, normally a symbiont of Drosophila, can be virulent, causing degeneration and early death.Proc Natl Acad Sci U S A941079210796
- 20. Starr DJ, Cline TW (2002) A host parasite interaction rescues Drosophila oogenesis defects. Nature 418: 76–79.DJ StarrTW Cline2002A host parasite interaction rescues Drosophila oogenesis defects.Nature4187679
- 21. Hoffman AA (1988) Partial cytoplasmic incompatibility between two Australian populations of Drosophila melanogaster. Entomol Exp Appl 48: 61–67.AA Hoffman1988Partial cytoplasmic incompatibility between two Australian populations of Drosophila melanogaster.Entomol Exp Appl486167
- 22. Reynolds KT, Hoffmann AA (2002) Male age, host effects and the weak expression or non-expression of cytoplasmic incompatibility in Drosophila strains infected by maternally transmitted Wolbachia. Genet Res 80: 79–87.KT ReynoldsAA Hoffmann2002Male age, host effects and the weak expression or non-expression of cytoplasmic incompatibility in Drosophila strains infected by maternally transmitted Wolbachia.Genet Res807987
- 23. Yamada R, Floate KD, Riegler M, O'Neill SL (2007) Male development time influences the strength of Wolbachia-induced cytoplasmic incompatibility expression in Drosophila melanogaster. Genetics 177: 801–808.R. YamadaKD FloateM. RieglerSL O'Neill2007Male development time influences the strength of Wolbachia-induced cytoplasmic incompatibility expression in Drosophila melanogaster.Genetics177801808
- 24. Hoffman AA, Turelli M, Simmons GM (1986) Unidirectional incompatibility between populations of Drosophila simulans. Evolution 40: 692–701.AA HoffmanM. TurelliGM Simmons1986Unidirectional incompatibility between populations of Drosophila simulans.Evolution40692701
- 25. Hoffmann AA, Hercus M, Dagher H (1998) Population dynamics of the Wolbachia infection causing cytoplasmic incompatibility in Drosophila melanogaster. Genetics 148: 221–231.AA HoffmannM. HercusH. Dagher1998Population dynamics of the Wolbachia infection causing cytoplasmic incompatibility in Drosophila melanogaster.Genetics148221231
- 26. Lipsitch M, Nowak MA, Ebert D, May RM (1995) The population dynamics of vertically and horizontally transmitted parasites. Proc Biol Sci 260: 321–327.M. LipsitchMA NowakD. EbertRM May1995The population dynamics of vertically and horizontally transmitted parasites.Proc Biol Sci260321327
- 27. Harcombe W, Hoffmann AA (2004) Wolbachia effects in Drosophila melanogaster: in search of fitness benefits. J Invertebr Pathol 87: 45–50.W. HarcombeAA Hoffmann2004Wolbachia effects in Drosophila melanogaster: in search of fitness benefits.J Invertebr Pathol874550
- 28. Olsen K, Reynolds KT, Hoffmann AA (2001) A field cage test of the effects of the endosymbiont Wolbachia on Drosophila melanogaster. Heredity 86: 731–737.K. OlsenKT ReynoldsAA Hoffmann2001A field cage test of the effects of the endosymbiont Wolbachia on Drosophila melanogaster.Heredity86731737
- 29. Fry AJ, Rand DM (2002) Wolbachia interactions that determine Drosophila melanogaster survival. Evolution 56: 1976–1981.AJ FryDM Rand2002Wolbachia interactions that determine Drosophila melanogaster survival.Evolution5619761981
- 30. Fry AJ, Palmer MR, Rand DM (2004) Variable fitness effects of Wolbachia infection in Drosophila melanogaster. Heredity 93: 379–389.AJ FryMR PalmerDM Rand2004Variable fitness effects of Wolbachia infection in Drosophila melanogaster.Heredity93379389
- 31. Montenegro H, Petherwick AS, Hurst GD, Klaczko LB (2006) Fitness effects of Wolbachia and Spiroplasma in Drosophila melanogaster. Genetica 127: 207–215.H. MontenegroAS PetherwickGD HurstLB Klaczko2006Fitness effects of Wolbachia and Spiroplasma in Drosophila melanogaster.Genetica127207215
- 32. Hoffmann JA (2003) The immune response of Drosophila. Nature 426: 33–38.JA Hoffmann2003The immune response of Drosophila.Nature4263338
- 33. Lemaitre B, Nicolas E, Michaut L, Reichhart JM, Hoffmann JA (1996) The dorsoventral regulatory gene cassette spatzle/Toll/cactus controls the potent antifungal response in Drosophila adults. Cell 86: 973–983.B. LemaitreE. NicolasL. MichautJM ReichhartJA Hoffmann1996The dorsoventral regulatory gene cassette spatzle/Toll/cactus controls the potent antifungal response in Drosophila adults.Cell86973983
- 34. Akira S, Uematsu S, Takeuchi O (2006) Pathogen recognition and innate immunity. Cell 124: 783–801.S. AkiraS. UematsuO. Takeuchi2006Pathogen recognition and innate immunity.Cell124783801
- 35. Lemaitre B, Hoffmann J (2007) The host defense of Drosophila melanogaster. Annu Rev Immunol 25: 697–743.B. LemaitreJ. Hoffmann2007The host defense of Drosophila melanogaster.Annu Rev Immunol25697743
- 36. Dostert C, Jouanguy E, Irving P, Troxler L, Galiana-Arnoux D, et al. (2005) The Jak-STAT signaling pathway is required but not sufficient for the antiviral response of drosophila. Nat Immunol 6: 946–953.C. DostertE. JouanguyP. IrvingL. TroxlerD. Galiana-Arnoux2005The Jak-STAT signaling pathway is required but not sufficient for the antiviral response of drosophila.Nat Immunol6946953
- 37. Takaoka A, Yanai H (2006) Interferon signalling network in innate defence. Cell Microbiol 8: 907–922.A. TakaokaH. Yanai2006Interferon signalling network in innate defence.Cell Microbiol8907922
- 38. Galiana-Arnoux D, Dostert C, Schneemann A, Hoffmann JA, Imler JL (2006) Essential function in vivo for Dicer-2 in host defense against RNA viruses in drosophila. Nat Immunol 7: 590–597.D. Galiana-ArnouxC. DostertA. SchneemannJA HoffmannJL Imler2006Essential function in vivo for Dicer-2 in host defense against RNA viruses in drosophila.Nat Immunol7590597
- 39. Wang XH, Aliyari R, Li WX, Li HW, Kim K, et al. (2006) RNA interference directs innate immunity against viruses in adult Drosophila. Science 312: 452–454.XH WangR. AliyariWX LiHW LiK. Kim2006RNA interference directs innate immunity against viruses in adult Drosophila.Science312452454
- 40. van Rij RP, Saleh MC, Berry B, Foo C, Houk A, et al. (2006) The RNA silencing endonuclease Argonaute 2 mediates specific antiviral immunity in Drosophila melanogaster. Genes Dev 20: 2985–2995.RP van RijMC SalehB. BerryC. FooA. Houk2006The RNA silencing endonuclease Argonaute 2 mediates specific antiviral immunity in Drosophila melanogaster.Genes Dev2029852995
- 41. Zambon RA, Vakharia VN, Wu LP (2006) RNAi is an antiviral immune response against a dsRNA virus in Drosophila melanogaster. Cell Microbiol 8: 880–889.RA ZambonVN VakhariaLP Wu2006RNAi is an antiviral immune response against a dsRNA virus in Drosophila melanogaster.Cell Microbiol8880889
- 42. Ryder E, Blows F, Ashburner M, Bautista-Llacer R, Coulson D, et al. (2004) The DrosDel collection: a set of P-element insertions for generating custom chromosomal aberrations in Drosophila melanogaster. Genetics 167: 797–813.E. RyderF. BlowsM. AshburnerR. Bautista-LlacerD. Coulson2004The DrosDel collection: a set of P-element insertions for generating custom chromosomal aberrations in Drosophila melanogaster.Genetics167797813
- 43. Brun G, Plus N (1978) The viruses of Drosophila. In: Ashburner M, Wright TRF, editors. The genetics and biology of Drosophila. New York: Academic Press. pp. 625–702.G. BrunN. Plus1978The viruses of Drosophila.In:. M. AshburnerTRF WrightThe genetics and biology of DrosophilaNew YorkAcademic Press625702
- 44. Johnson KN, Christian PD (1998) The novel genome organization of the insect picorna-like virus Drosophila C virus suggests this virus belongs to a previously undescribed virus family. J Gen Virol 79: 191–203.KN JohnsonPD Christian1998The novel genome organization of the insect picorna-like virus Drosophila C virus suggests this virus belongs to a previously undescribed virus family.J Gen Virol79191203
- 45. Zhou W, Rousset F, O'Neil S (1998) Phylogeny and PCR-based classification of Wolbachia strains using wsp gene sequences. Proc Biol Sci 265: 509–515.W. ZhouF. RoussetS. O'Neil1998Phylogeny and PCR-based classification of Wolbachia strains using wsp gene sequences.Proc Biol Sci265509515
- 46. Wu M, Sun LV, Vamathevan J, Riegler M, DeBoy R, et al. (2004) Phylogenomics of the reproductive parasite Wolbachia pipientis wMel: A streamlined genome overrun by mobile genetic elements. PLoS Biol 2(3): 327–341.M. WuLV SunJ. VamathevanM. RieglerR. DeBoy2004Phylogenomics of the reproductive parasite Wolbachia pipientis wMel: A streamlined genome overrun by mobile genetic elements.PLoS Biol2(3)327341
- 47. Mateos M, Castrezana SJ, Nankivell BJ, Estes AM, Markow TA, et al. (2006) Heritable endosymbionts of Drosophila. Genetics 174: 363–376.M. MateosSJ CastrezanaBJ NankivellAM EstesTA Markow2006Heritable endosymbionts of Drosophila.Genetics174363376
- 48. Montenegro H, Solferini V, Klaczko L, Hurst G (2005) Male-killing Spiroplasma naturally infecting Drosophila melanogaster. Insect Mol Biol 14: 281–287.H. MontenegroV. SolferiniL. KlaczkoG. Hurst2005Male-killing Spiroplasma naturally infecting Drosophila melanogaster.Insect Mol Biol14281287
- 49. Habayeb MS, Ekengren SK, Hultmark D (2006) Nora virus, a persistent virus in Drosophila, defines a new picorna-like virus family. J Gen Virol 87: 3045–3051.MS HabayebSK EkengrenD. Hultmark2006Nora virus, a persistent virus in Drosophila, defines a new picorna-like virus family.J Gen Virol8730453051
- 50. Ball LA, Johnson KL (1998) Nodaviruses of Iisects. In: Miller LK, Ball LA, editors. The insect viruses. New York: Plenum Press. pp. 225–261.LA BallKL Johnson1998Nodaviruses of Iisects.In:. LK MillerLA BallThe insect virusesNew YorkPlenum Press225261
- 51. Dearing SC, Scotti PD, Wigley PJ, Dhana SD (1980) A small RNA virus isolated from the grass grub, Costelytra zealandica (Coleoptera, Scarabaeidae). New Zeal J Zool 7: 267–269.SC DearingPD ScottiPJ WigleySD Dhana1980A small RNA virus isolated from the grass grub, Costelytra zealandica (Coleoptera, Scarabaeidae).New Zeal J Zool7267269
- 52. Williams T (1998) Invertebrate iridescent viruses. In: Miller LK, Ball LA, editors. The insect viruses. New York: Plenum Press. pp. 225–261.T. Williams1998Invertebrate iridescent viruses.In:. LK MillerLA BallThe insect virusesNew YorkPlenum Press225261
- 53. Jakob NJ, Kleespies RG, Tidona CA, Müller K, Gelderblom HR, et al. (2002) Comparative analysis of the genome and host range characteristics of two insect iridoviruses: Chilo iridescent virus and a cricket iridovirus isolate. J Gen Virol 83: 463–470.NJ JakobRG KleespiesCA TidonaK. MüllerHR Gelderblom2002Comparative analysis of the genome and host range characteristics of two insect iridoviruses: Chilo iridescent virus and a cricket iridovirus isolate.J Gen Virol83463470
- 54. Constantino M, Christian P, Marina CF, Williams T (2001) A comparison of techniques for detecting Invertebrate iridescent virus 6. J Virol Methods 98: 109–118.M. ConstantinoP. ChristianCF MarinaT. Williams2001A comparison of techniques for detecting Invertebrate iridescent virus 6.J Virol Methods98109118
- 55. Chung HK, Kordyban S, Cameron L, Dobos P (1996) Sequence analysis of the bicistronic Drosophila X virus genome segment A and its encoded polypeptides. Virology 225: 359–368.HK ChungS. KordybanL. CameronP. Dobos1996Sequence analysis of the bicistronic Drosophila X virus genome segment A and its encoded polypeptides.Virology225359368
- 56. Dobson SL, Bourtzis K, Braig HR, Jones BF, Zhou W, et al. (1999) Wolbachia infections are distributed throughout insect somatic and germ line tissues. Insect Biochem Mol Biol 29: 153–160.SL DobsonK. BourtzisHR BraigBF JonesW. Zhou1999Wolbachia infections are distributed throughout insect somatic and germ line tissues.Insect Biochem Mol Biol29153160
- 57. Cherry S, Doukas T, Armknecht S, Whelan S, Wang H, et al. (2005) Genome-wide RNAi screen reveals a specific sensitivity of IRES-containing RNA viruses to host translation inhibition. Genes Dev 19: 445–452.S. CherryT. DoukasS. ArmknechtS. WhelanH. Wang2005Genome-wide RNAi screen reveals a specific sensitivity of IRES-containing RNA viruses to host translation inhibition.Genes Dev19445452
- 58. Iturbe-Ormaetxe I, Burke GR, Riegler M, O'Neill SL (2005) Distribution, expression, and motif variability of ankyrin domain genes in Wolbachia pipientis. J Bacteriol 187: 5136–5145.I. Iturbe-OrmaetxeGR BurkeM. RieglerSL O'Neill2005Distribution, expression, and motif variability of ankyrin domain genes in Wolbachia pipientis.J Bacteriol18751365145
- 59. Barton ES, White DW, Cathelyn JS, Brett-McClellan KA, Engle M, et al. (2007) Herpesvirus latency confers symbiotic protection from bacterial infection. Nature 447: 326–329.ES BartonDW WhiteJS CathelynKA Brett-McClellanM. Engle2007Herpesvirus latency confers symbiotic protection from bacterial infection.Nature447326329
- 60. Xi Z, Ramirez JL, Dimopoulos G (2008) The Aedes aegypti Toll pathway controls Dengue virus infection. PLoS Pathog 4(7): e1000098.. Z. XiJL RamirezG. Dimopoulos2008The Aedes aegypti Toll pathway controls Dengue virus infection.PLoS Pathog4(7)e1000098.
- 61. Xi Z, Gavotte L, Xie Y, Dobson SL (2008) Genome-wide analysis of the interaction between the endosymbiotic bacterium Wolbachia and its Drosophila host. BMC Genomics 9: 1.Z. XiL. GavotteY. XieSL Dobson2008Genome-wide analysis of the interaction between the endosymbiotic bacterium Wolbachia and its Drosophila host.BMC Genomics91
- 62. Panteleev DIu, Goriacheva II, Andrianov BV, Reznik NL, Lazebnyĭ OE, et al. (2007) The endosymbiotic bacterium Wolbachia enhances the nonspecific resistance to insect pathogens and alters behavior of Drosophila melanogaster. Genetika 43: 1277–1280.DIu PanteleevII GoriachevaBV AndrianovNL ReznikOE Lazebnyĭ2007The endosymbiotic bacterium Wolbachia enhances the nonspecific resistance to insect pathogens and alters behavior of Drosophila melanogaster.Genetika4312771280
- 63. Bourtzis K, Pettigrew MM, O'Neill SL (2000) Wolbachia neither induces nor suppresses transcripts encoding antimicrobial peptides. Insect Mol Biol 9: 635–639.K. BourtzisMM PettigrewSL O'Neill2000Wolbachia neither induces nor suppresses transcripts encoding antimicrobial peptides.Insect Mol Biol9635639
- 64. Fytrou A, Schofield PG, Kraaijeveld AR, Hubbard SF (2006) Wolbachia infection suppresses both host defence and parasitoid counter-defence. Proc Biol Sci 273: 791–796.A. FytrouPG SchofieldAR KraaijeveldSF Hubbard2006Wolbachia infection suppresses both host defence and parasitoid counter-defence.Proc Biol Sci273791796
- 65. Goto S, Anbutsu H, Fukatsu T (2006) Asymmetrical interactions between Wolbachia and Spiroplasma endosymbionts coexisting in the same insect host. Appl Environ Microbiol 72: 4805–4810.S. GotoH. AnbutsuT. Fukatsu2006Asymmetrical interactions between Wolbachia and Spiroplasma endosymbionts coexisting in the same insect host.Appl Environ Microbiol7248054810
- 66. Settles EW, Friesen PD (2008) Flock house virus induces apoptosis by depletion of Drosophila inhibitor-of-apoptosis protein DIAP1. J Virol 82: 1378–1388.EW SettlesPD Friesen2008Flock house virus induces apoptosis by depletion of Drosophila inhibitor-of-apoptosis protein DIAP1.J Virol8213781388
- 67. Bazzocchi C, Comazzi S, Santoni R, Bandi C, Genchi C, et al. (2007) Wolbachia surface protein (WSP) inhibits apoptosis in human neutrophils. Parasite Immunol 29: 73–79.C. BazzocchiS. ComazziR. SantoniC. BandiC. Genchi2007Wolbachia surface protein (WSP) inhibits apoptosis in human neutrophils.Parasite Immunol297379
- 68. Grivel JC, Ito Y, Fagà G, Santoro F, Shaheen F, et al. (2001) Suppression of CCR5- but not CXCR4-tropic HIV-1 in lymphoid tissue by human herpesvirus 6. Nat Med 7: 1232–1235.JC GrivelY. ItoG. FagàF. SantoroF. Shaheen2001Suppression of CCR5- but not CXCR4-tropic HIV-1 in lymphoid tissue by human herpesvirus 6.Nat Med712321235
- 69. Scarborough CL, Ferrari J, Godfray HC (2005) Aphid protected from pathogen by endosymbiont. Science 310: 1781.CL ScarboroughJ. FerrariHC Godfray2005Aphid protected from pathogen by endosymbiont.Science3101781
- 70. Gil-Turnes MS, Hay ME, Fenical W (1989) Symbiotic marine bacteria chemically defend crustacean embryos from a pathogenic fungus. Science 246: 116–118.MS Gil-TurnesME HayW. Fenical1989Symbiotic marine bacteria chemically defend crustacean embryos from a pathogenic fungus.Science246116118
- 71. Oliver KM, Russell JA, Moran NA, Hunter MS (2003) Facultative bacterial symbionts in aphids confer resistance to parasitic wasps. Proc Natl Acad Sci U S A 100: 1803–1807.KM OliverJA RussellNA MoranMS Hunter2003Facultative bacterial symbionts in aphids confer resistance to parasitic wasps.Proc Natl Acad Sci U S A10018031807
- 72. Kaltenpoth M, Göttler W, Herzner G, Strohm E (2005) Symbiotic bacteria protect wasp larvae from fungal infestation. Curr Biol 15: 475–479.M. KaltenpothW. GöttlerG. HerznerE. Strohm2005Symbiotic bacteria protect wasp larvae from fungal infestation.Curr Biol15475479
- 73. Xi Z, Khoo CC, Dobson SL (2005) Wolbachia establishment and invasion in an Aedes aegypti laboratory population. Science 310: 326–328.Z. XiCC KhooSL Dobson2005Wolbachia establishment and invasion in an Aedes aegypti laboratory population.Science310326328
- 74. Brownstein JS, Hett E, O'Neill SL (2003) The potential of virulent Wolbachia to modulate disease transmission by insects. J Invertebr Pathol 84: 24–29.JS BrownsteinE. HettSL O'Neill2003The potential of virulent Wolbachia to modulate disease transmission by insects.J Invertebr Pathol842429
- 75. Hedges LM, Brownlie JC, O'Neill SL, Johnson KN (2008) Wolbachia and virus protection in insects. Science 322: 702.LM HedgesJC BrownlieSL O'NeillKN Johnson2008Wolbachia and virus protection in insects.Science322702
- 76. Pasyukova EG, Nuzhdin SV (1993) Doc and copia instability in an isogenic Drosophila melanogaster stock. Mol Gen Genet 240: 302–306.EG PasyukovaSV Nuzhdin1993Doc and copia instability in an isogenic Drosophila melanogaster stock.Mol Gen Genet240302306
- 77. Jousset FX, Plus N, Croizier G, Thomas M (1972) Existence chez Drosophila de deux groupes de Picornavirus de proprietés sérologiques et biologiques différentes. C R Acad Sci Hebd Seances Acad Sci D 275: 3043–3046.FX JoussetN. PlusG. CroizierM. Thomas1972Existence chez Drosophila de deux groupes de Picornavirus de proprietés sérologiques et biologiques différentes.C R Acad Sci Hebd Seances Acad Sci D27530433046
- 78. Johnson KN, Christian PD (1999) Molecular characterization of Drosophila C virus isolates. J Invertebr Pathol 73: 248–254.KN JohnsonPD Christian1999Molecular characterization of Drosophila C virus isolates.J Invertebr Pathol73248254
- 79. Fukaya M, Nasu S (1966) A Chilo iridescent virus (CIV) from the rice stem borer, Chilo suppressalis Walker (Lepidoptera: Pyralidae). Appl Entomol Zool 1: 69–72.M. FukayaS. Nasu1966A Chilo iridescent virus (CIV) from the rice stem borer, Chilo suppressalis Walker (Lepidoptera: Pyralidae).Appl Entomol Zool16972
- 80. Reed LJ, Muench H (1938) A simple method of estimating fifty per cent endpoints. Am J Epidemiol 27: 493.LJ ReedH. Muench1938A simple method of estimating fifty per cent endpoints.Am J Epidemiol27493
- 81. Chu D, Klymkowsky M (1989) The appearance of acetylated alpha-tubulin during early development and cellular differentiation in Xenopus. Dev Biol 136: 104–117.D. ChuM. Klymkowsky1989The appearance of acetylated alpha-tubulin during early development and cellular differentiation in Xenopus.Dev Biol136104117
- 82. Larkin MA, Blackshields G, Brown NP, Chenna R, McGettigan PA, et al. (2007) Clustal W and Clustal X version 2.0. Bioinformatics 23: 2947–2948.MA LarkinG. BlackshieldsNP BrownR. ChennaPA McGettigan2007Clustal W and Clustal X version 2.0.Bioinformatics2329472948