Citation: Tsukaya H (2008) Controlling Size in Multicellular Organs: Focus on the Leaf. PLoS Biol6(7): e174. https://doi.org/10.1371/journal.pbio.0060174
Published: July 15, 2008
Copyright: © 2008 Hirokazu Tsukaya. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: This work was supported by Grants-in-Aid for Creative Scientific Research, for Scientific Research A, and for Scientific Research on Priority Areas, from the Japan Society for the Promotion of Science and from the Toray Science Foundation.
Abbreviations: o/x, over-expressor; wt, wild-type
Leaf size depends on cell number and size. However, leaves are not simply the sum of cell size and number; rather, they are under the control of an unknown, organ-wide integration system. The existence of such a system is strongly suggested by two mysterious phenomena: compensation and high-ploidy syndrome. Compensation is characterized by cell enlargement triggered by a significant decrease in cellular proliferation, while plants with high-ploidy syndrome have more than eight sets of homologous chromosomes (8C), resulting in an increase in cell volume, but smaller leaves. Determining the mechanisms underlying these phenomena will provide important insight into the mechanism of multicellular organogenesis.
What Drives Compensation?
In 1998, I noticed an unusual phenomenon in plants involving a decrease in the number of cells in the leaf lamina with a consequent increase in cell volume. I called this phenomenon “Hoshosayou,” which is Japanese for “compensation” , because I considered the inverse relationship (a decrease in cell number with an increase in cell volume) to be the most important feature, although the effect on leaf area was often incomplete. At that time, a limited number of examples were available to support the existence of such a relationship; however, additional evidence continues to accumulate. In 2002, having become convinced of the generality of the relationship, I adopted the English word “compensation” . Gerrit Beemster of Ghent University (Belgium) also named this phenomenon compensation the following year . We have since found that compensation occurs specifically in leaves and related lateral organs with a determinate fate (i.e., those organs that are under strict control in terms of when organogenesis starts and finishes), and not in indeterminate organs such as roots . This likely reflects the presence of an unknown integration system that oversees cell division and expansion in determinate organs . The details of this system are, at present, entirely unknown. Understanding the mechanism of multicellular organogenesis, many aspects of which remain mysterious, is one of the major goals of modern biology.
The mechanisms that integrate cellular proliferation with organ size in animals are also not yet understood [6,7]. As is widely known (even in Greek mythology), the size of the human liver is strictly controlled, and if most of the liver is surgically removed, it will regenerate to the proper size. Researchers have hypothesized a “total-mass checkpoint” mechanism(s) to explain this phenomenon , but, again, the molecular details are unknown.
Interestingly, several lines of evidence indicate that the mechanisms underlying the control of leaf size appear to differ from the total-mass checkpoint mechanisms. First, whereas a total-mass checkpoint in animals would prohibit organs larger than a standard size, leaves can become much larger than the standard size if cell division or expansion is enhanced [2,8–11]. Second, the trade-off-like relationship between number and size in leaf cells (i.e., compensation) is exhibited only when cell division is significantly decreased. However, a decrease in cell number does not always increase cell expansion [12–15], and such a response does not occur when cell expansion alone is altered [16–19]. A unidirectional relationship like this is unknown in animal organs. Thus, the question remains: what is the mechanism behind compensation?
Compensation Rule Enigmas
When I talk about compensation at a seminar or symposium, nearly half of the audience responds by saying, “It should be a simple result of retarded cell division. If you cut a cake (or cell) in two, each piece is much larger than when you cut the cake (cell) in four.” In other words, they see compensation as a simple result of retarded division and not a unique phenomenon. This is not the case, however. The mechanisms involved in the coordination of cell growth and division are not simple, even in a budding yeast cell . Let us examine some of the clues obtained from leaf development studies in the model plant Arabidopsis thaliana (referred to hereafter as Arabidopsis).
First, compensation cannot always “compensate” for the total mass or overall leaf shape and size. Arabidopsis plants over-expressing KIP-Related Protein 2 (KRP2) exhibit the typical features of compensation (smaller leaves containing fewer larger cells) [4,21], but they fail to maintain the overall size or shape of the leaves (Figure 1). Similarly, a series of mutants (the so-called fugu mutants) that typically exhibit compensation show significant variation in leaf size and shape .
(A) Gross morphology of the sixth leaf of wild-type Columbia (wt: left) and KRP2-over-expressor (o/x: right) plants. There are significant differences in the size and shape of the leaves. Scale bar, 1 mm. (B) A paradermal view of the first layer of palisade tissue from the leaves shown in panel (A): Left, wt Columbia, and right, KRP2-o/x. Scale bar, 100 μm. Note the increase in cell size (a result of compensated cell enlargement) in the KPR2-o/x.
Second, abnormally enhanced cell expansion (hereafter referred to as compensated cell enlargement) [4,19] is not observed during the developmental stage in which cell division actually occurs . Compensated cell enlargement depends on a significant increase in the vacuolar volume (often ten times the cytoplasmic volume), and begins only after cell division ceases. Thus, compensation is not a passive result of decreased cell division (as in the cake analogy), but a result of unknown positive, emergent control mechanisms.
Third, compensation is not always observed when cell division declines in the leaf primordium. Large-scale screening of Arabidopsis leaf size/shape mutants found several mutants with a moderately low number of leaf cells but without compensated cell enlargement [12,13]. A reduction in cell number caused by the over-expression of the BIG BROTHER E3 ubiquitin ligase gene results in even smaller leaf cells . Thus, compensation is not a simple trade-off between cell division and expansion in an organ of a given size.
Fourth, as mentioned above, changes in cell size are not observed when cell division is enhanced. For example, the loss-of-function mutation ANGUSTIFOLIA3/GIF1 (AN3/GIF1) typically causes compensation (a shortened period of cell division that results in a decreased number of cells and increased cell expansion). However, over-expression of the same gene simply increases the period of cell division and the number of leaf cells without causing any abnormality in cell size . A similar phenomenon has been reported for several other genes, including AINTEGUMENTA .
Fifth, we recently found that there may be a threshold decrease in cell division that triggers compensated cell enlargement (U. Fujikura, G. Horiguchi, and H. Tsukaya, unpublished data). Moreover, compensated cell enlargement is observed in the tip-most region of leaf primordia soon after the cells in the region stop dividing, whereas active cell division still occurs in the basal regions at this stage . This fact strongly suggests that compensated cell enlargement is an expression of some emergent control of cell expansion that is not linked to the final number of cells in the primordium, but to some component (e.g., level or speed) of cellular proliferation. How, then, are they linked? Does the trigger for compensation function in a non-cell-autonomous or cell-autonomous manner? We do not yet know the answers to these questions, but a recent study revealed that only a subset of the genetic pathways involved in leaf-cell expansion, which are used in normal leaf growth, are enhanced when compensated cell enlargement is triggered . This fact is an important clue as to the mechanism of compensation. The construction and analysis of chimeras for AN3 expression in leaf primordia should enhance our understanding of compensation, a big mystery that must be solved.
High-Ploidy Syndrome Offers Clues to the Link between Ploidy and Organ Size
Another curious phenomenon in the organ-wide control of plant organ size is the link between ploidy and organ size. High-ploidy syndrome offers clues to this link. Colchicine-induced polyploidization has been widely used for horticultural improvement because tetraploid plants (described as 4C) are generally larger than the parental diploid (2C) plants. This effect on body size is attributable to increases in cell size . Tetraploid Arabidopsis plants (4C = 20: four chromosomes of each type) are larger than their diploid parents (2C = 10) in terms of leaf, flower, and cell size in the leaf lamina (Figure 2).
Diploid, tetraploid, and octaploid Arabidopsis Columbia plants, two weeks after sowing, are shown from left to right. (A) Gross morphology of the plantlets. The octaploid plant has smaller leaves compared to the diploid and tetraploid plants. c, cotyledon. Unit of scale, 1 mm. (B) Paradermal view of the palisade cells in the first leaves. From left to right, diploid, tetraploid, and octaploid. Bar, 100 μm.
In addition, annual weeds such as Arabidopsis frequently exhibit repeated endoreduplication that results in cell-autonomous genome duplication during such developmental processes as trichome differentiation and increases in epidermal cell volume [27,28]. Endoreduplication is a modified version of the cell cycle that lacks M phase. During each round of endoreduplication, the genome is duplicated, but the number of chromosomes stays the same, resulting in polyteny (a condition in which the replicated chromosomes do not separate). Do these two methods of achieving polyploidy (i.e., increasing the number of chromosomes versus polyteny) work in the same way to increase cell volume?
We recently showed that tetraploidization partially rescues the cell-size defect in endoreduplication-deficient mutants, suggesting that cell size depends on genome size, regardless of the status of each chromosome (Figure 3) . Thus, the answer seems to be yes, at least in part, although we do not know how ploidy and cell size are linked. A popular answer to this question is that increases in ploidy automatically and proportionally increase the amount of cellular components, resulting in larger cells. If this were true, cell volume after tetraploidization should be doubled, independent of mutations in cell size control. But fact refutes this prediction: the 4C/2C cell volume ratio varies among different strains (H. Tsukaya, unpublished data). Moreover, some reports have suggested that changes in ploidy do not affect cell size in particular organs . Thus, why and how ploidy affects cell volume is entirely unknown .
Tetraploidization partially recovered the dwarfism observed in the diploid bin4 mutant. See  for further details. Unit of scale, 1 mm.
The relationship between ploidy and body/organ size is complex. As mentioned above, tetraploid plant organs/bodies are generally larger than diploid ones, and hexaploids are generally larger than tetraploids. However, octaploid plant organs/bodies are the same size as those in hexaploids, or sometimes much smaller than even those in diploids [31–33]; observations of octaploid Arabidopsis plants confirm this general rule (Figure 2). The leaf cells of octaploid plants are larger than those of tetraploids, whereas the leaves of octaploids are much smaller than those of tetraploids, suggesting that octaploids have fewer cells per leaf. Thus, higher ploidy enhances cell expansion but inhibits leaf cell division, leaving us with the “high-ploidy syndrome” dilemma.
This type of trade-off has long been known, even in animals . Based on studies of 1C, 2C, 3C, 5C, and 7C salamanders, Fankhauser (1945) described the trade-off between cell volume and organ/body size as “compensation” . For example, haploid salamanders have smaller cells than diploids, but the reduction is “completely compensated by [an] increase in cell number” . Likewise, tetraploid salamanders have fewer but larger cells than do diploids . A similar trade-off occurs in tetraploid mice . The compensation observed in polyploid animals could be interpreted as the result of a strict total-mass checkpoint. Is this also true for the high-ploidy syndrome observed in plants? The answer is no because the presence of a total-mass checkpoint is not supported in plants, in which increases in cell volume are simply reflected in organ size (except for the above-mentioned cases in which severe decreases in cell division result in compensation) [2,9,11]. Is a total-mass checkpoint effective only in plants with a ploidy higher than 8C?
One possible explanation for high-ploidy syndrome is that at higher ploidy levels the cell cycle becomes more costly, so cell division slows , resulting in fewer than the expected number of cells and smaller organs. In fact, if tetraploid Arabidopsis seeds are sown together with diploid seeds, the tetraploids grow slightly more slowly in terms of the number of days to flowering and plastochron (see Figures 2 and 3), although the leaves and flowers of the mature tetraploids are much larger than those of the mature diploids. This explanation, however, contradicts the popular belief that increases in ploidy automatically and proportionally increase the amount of cellular components. If tetraploid cells are twice as rich as diploid cells in terms of their cellular components, the rate of cell growth should be the same in diploid and tetraploid cells. Why, then, do tetraploid organs expand more slowly than diploid ones?
One plausible answer is that an increase in ploidy alters the ratios between the various components of the cell with different dimensions, adding stress to cell activity . For example, kinetochore attachment to the spindle pole body was altered in a tetraploid budding yeast in comparison to the diploid strain . This alteration can be explained by an imbalance between the increase in surface area of the spindle pole body (bidimensional) and the length of the spindle (unidimensional). Even so, the answer above cannot explain why hexaploidy is the threshold for a proportional relationship between ploidy and organ size in plants. In addition, the length of the cell cycle is not altered by an increase in ploidy in unicellular budding yeast . Apparently, there are rules that control organ size at the organ level; however, what these rules are remains, at present, an unsolved mystery.
How can we solve this mystery? The imbalance in mitosis in tetraploid budding yeast was identified from a genome-wide screen for mutants with ploidy-specific lethality . Although Arabidopsis is not as suitable for the types of whole-genome-wide mutational approaches taken in budding yeast, mutational studies involving specific genes are easy. Moreover, leaves are produced repeatedly in plants and are easily analyzed; thus, leaves are a good model system for studies of the mechanisms underlying multicellular, determinate organ development. In fact, a series of tetraploid Arabidopsis mutants deduced from diploid single-gene mutants are being constructed in my laboratory, and have already yielded several clues as to the role of ploidy in cell/organ size control . It is my hope that other researchers will focus on the mysteries surrounding the development of this simple but curious organ so that they may be resolved in the near future.
I thank the members of my laboratories at the University of Tokyo and the National Institute for Basic Biology (NIBB) for helpful discussions. I am grateful for the collaborative efforts of Drs. Gerrit Beemster of Ghent University, Belgium, and Keiko Sugimoto-Shirasu of RIKEN, Japan, on the topics discussed. Thanks to Drs. Mitsuyasu Hasebe of NIBB, Japan, and Misako Mishima for providing the octaploid Arabidopsis seeds (Columbia background).
- 1. (1998) Relationship between shape of cells and shape of the organ. In: Imazeki H, Shibaoka H, editors. Phytohormones and cell shape. Tokyo: Gakkai-shuppann Center. pp. 177–184.H. Tsukaya1998Relationship between shape of cells and shape of the organ.In. H. ImazekiH. Shibaokaeditors. Phytohormones and cell shapeTokyoGakkai-shuppann Center177184[In Japanese.]. [In Japanese.].
- 2. Tsukaya H (2002) Interpretation of mutants in leaf morphology: Genetic evidence for a compensatory system in leaf morphogenesis that provides a new link between cell and organismal theory. Int Rev Cytol 217: 1–39.H. Tsukaya2002Interpretation of mutants in leaf morphology: Genetic evidence for a compensatory system in leaf morphogenesis that provides a new link between cell and organismal theory.Int Rev Cytol217139
- 3. Beemster GT, Fiorani F, Inzé D (2003) Cell cycle: The key to plant growth control. Trends Plant Sci 8: 154–158.GT BeemsterF. FioraniD. Inzé2003Cell cycle: The key to plant growth control.Trends Plant Sci8154158
- 4. Ferjani A, Horiguchi G, Tsukaya H (2007) Analysis of leaf development in fugu mutants of Arabidopsis thaliana reveals three compensation modes that modulate cell expansion in determinate organs. Plant Physiol 144: 988–999.A. FerjaniG. HoriguchiH. Tsukaya2007Analysis of leaf development in fugu mutants of Arabidopsis thaliana reveals three compensation modes that modulate cell expansion in determinate organs.Plant Physiol144988999
- 5. Tsukaya H, Beemster G (2006) Genetics, cell cycle and cell expansion in organogenesis in plants. J Plant Res 119: 1–4.H. TsukayaG. Beemster2006Genetics, cell cycle and cell expansion in organogenesis in plants.J Plant Res11914
- 6. Conlon I, Raff M (1999) Size control in animal development. Cell 96: 235–244.I. ConlonM. Raff1999Size control in animal development.Cell96235244
- 7. Potter CJ, Xu T (2001) Mechanisms of size control. Curr Opin Genet Dev 11: 279–286.CJ PotterT. Xu2001Mechanisms of size control.Curr Opin Genet Dev11279286
- 8. (2002b) Leaf development. In: Somerville CR, Meyerowitz EM, editors. The Arabidopsis book. American Society of Plant Biologists. H. Tsukaya2002bLeaf development.In. CR SomervilleEM Meyerowitzeditors. The Arabidopsis book. American Society of Plant BiologistsAvailable http://www.aspb.org/downloads/arabidopsis/tsukaya.pdf. Accessed 9 June 2008. doi:10.1199/tab.0072. Available http://www.aspb.org/downloads/arabidopsis/tsukaya.pdf. Accessed 9 June 2008. doi:10.1199/tab.0072.
- 9. Tsukaya H (2003) Organ shape and size: A lesson from studies of leaf morphogenesis. Curr Opin Plant Biol 6: 57–62.H. Tsukaya2003Organ shape and size: A lesson from studies of leaf morphogenesis.Curr Opin Plant Biol65762
- 10. Tsukaya H (2005) Leaf shape: Genetic controls and environmental factors. Int J Dev Biol 49: 547–555.H. Tsukaya2005Leaf shape: Genetic controls and environmental factors.Int J Dev Biol49547555
- 11. Tsukaya H (2006) Mechanism of leaf shape determination. Ann Rev Plant Biol 57: 477–496.H. Tsukaya2006Mechanism of leaf shape determination.Ann Rev Plant Biol57477496
- 12. Narita NN, Moore S, Horiguchi G, Kubo M, Demura T, et al. (2004) Over-expression of a novel small peptide ROTUNDIFOLIA4 decreases cell proliferation and alters leaf shape in Arabidopsis. Plant J 38: 699–713.NN NaritaS. MooreG. HoriguchiM. KuboT. Demura2004Over-expression of a novel small peptide ROTUNDIFOLIA4 decreases cell proliferation and alters leaf shape in Arabidopsis.Plant J38699713
- 13. Horiguchi G, Ferjani A, Fujikura U, Tsukaya H (2006) Coordination of cell proliferation and cell expansion in the control of leaf size in Arabidopsis thaliana. J Plant Res 119: 37–42.G. HoriguchiA. FerjaniU. FujikuraH. Tsukaya2006Coordination of cell proliferation and cell expansion in the control of leaf size in Arabidopsis thaliana.J Plant Res1193742
- 14. Horiguchi G, Fujikura U, Ferjani A, Ishikawa N, Tsukaya H (2006) Large-scale histological analysis of leaf mutants using two simple leaf observation methods: Identification of novel genetic pathways governing the size and shape of leaves. Plant J 48: 638–644.G. HoriguchiU. FujikuraA. FerjaniN. IshikawaH. Tsukaya2006Large-scale histological analysis of leaf mutants using two simple leaf observation methods: Identification of novel genetic pathways governing the size and shape of leaves.Plant J48638644
- 15. Anastasiou E, Lenhard M (2007) Growing up to one's standard. Curr Opin Plant Biol 10: 63–69.E. AnastasiouM. Lenhard2007Growing up to one's standard.Curr Opin Plant Biol106369
- 16. Tsuge T, Tsukaya H, Uchimiya H (1996) Two independent and polarized processes of cell elongation regulate leaf blade expansion in Arabidopsis thaliana (L.) Heynh. Development 122: 1589–1600.T. TsugeH. TsukayaH. Uchimiya1996Two independent and polarized processes of cell elongation regulate leaf blade expansion in Arabidopsis thaliana (L.) Heynh.Development12215891600
- 17. Kim G-T, Tsukaya H, Uchimiya H (1998) The ROTUNDIFOLIA3 gene of Arabidopsis thaliana encodes a new member of the cytochrome P450 family that is required for the regulated polar elongation of leaf cells. Genes Dev 12: 2381–2391.G-T KimH. TsukayaH. Uchimiya1998The ROTUNDIFOLIA3 gene of Arabidopsis thaliana encodes a new member of the cytochrome P450 family that is required for the regulated polar elongation of leaf cells.Genes Dev1223812391
- 18. Kim G-T, Shoda K, Tsuge T, Cho K-H, Uchimiya H, et al. (2002) The ANGUSTIFOLIA gene of Arabidopsis, a plant CtBP gene, regulates leaf-cell expansion, the arrangement of cortical microtubules in leaf cells and expression of a gene involved in cell-wall formation. EMBO J 21: 1267–1279.G-T KimK. ShodaT. TsugeK-H ChoH. Uchimiya2002The ANGUSTIFOLIA gene of Arabidopsis, a plant CtBP gene, regulates leaf-cell expansion, the arrangement of cortical microtubules in leaf cells and expression of a gene involved in cell-wall formation.EMBO J2112671279
- 19. Fujikura U, Horiguchi G, Tsukaya H (2007) Dissection of enhanced cell expansion processes in leaves triggered by defect in cell proliferation, with reference to roles of endoreduplication. Plant Cell Physiol 48: 278–286.U. FujikuraG. HoriguchiH. Tsukaya2007Dissection of enhanced cell expansion processes in leaves triggered by defect in cell proliferation, with reference to roles of endoreduplication.Plant Cell Physiol48278286
- 20. Jorgensen P, Tyers M (2004) How cells coordinate growth and division. Curr Biol 14: R1014–R1027.P. JorgensenM. Tyers2004How cells coordinate growth and division.Curr Biol14R1014R1027
- 21. De Veylder L, Beeckman T, Beemster GTS, Krols L, Terras F, et al. (2001) Functional analysis of cyclin-dependent kinase inhibitors of Arabidopsis. Plant Cell 13: 1653–1667.L. De VeylderT. BeeckmanGTS BeemsterL. KrolsF. Terras2001Functional analysis of cyclin-dependent kinase inhibitors of Arabidopsis.Plant Cell1316531667
- 22. Disch S, Anastasiou E, Sharma VK, Laux T, Fletcher JC, et al. (2006) The E3 ubiquitin ligase BIG BROTHER controls Arabidopsis organ size in a dosage-dependent manner. Curr Biol 16: 272–279.S. DischE. AnastasiouVK SharmaT. LauxJC Fletcher2006The E3 ubiquitin ligase BIG BROTHER controls Arabidopsis organ size in a dosage-dependent manner.Curr Biol16272279
- 23. Horiguchi G, Kim G-T, Tsukaya H (2005) The transcription factor AtGRF5 and the transcription coactivator AN3 regulate cell proliferation in leaf primordia of Arabidopsis thaliana. Plant J 43: 68–78.G. HoriguchiG-T KimH. Tsukaya2005The transcription factor AtGRF5 and the transcription coactivator AN3 regulate cell proliferation in leaf primordia of Arabidopsis thaliana.Plant J436878
- 24. Mizukami Y, Fischer RL (2000) Plant organ size control: AINTEGUMENTA regulates growth and cell numbers during organogenesis. Proc Natl Acad Sci U S A 97: 942–947.Y. MizukamiRL Fischer2000Plant organ size control: AINTEGUMENTA regulates growth and cell numbers during organogenesis.Proc Natl Acad Sci U S A97942947
- 25. Fujikura U, Horiguchi G, Tsukaya H (2007) Genetic relationship between angustifolia3 and extra-small sisters highlights novel mechanisms controlling leaf size. Plant Signal Behav 2: 378–380.U. FujikuraG. HoriguchiH. Tsukaya2007Genetic relationship between angustifolia3 and extra-small sisters highlights novel mechanisms controlling leaf size.Plant Signal Behav2378380
- 26. Müntzing A (1936) The evolutionary significance of autopolyploidy. Hereditas 21: 263–378.A. Müntzing1936The evolutionary significance of autopolyploidy.Hereditas21263378
- 27. Melaragno JE, Mehrotra B, Coleman AW (1993) Relationship between endopolyploidy and cell size in epidermal tissue of Arabidopsis. Plant Cell 5: 1661–1668.JE MelaragnoB. MehrotraAW Coleman1993Relationship between endopolyploidy and cell size in epidermal tissue of Arabidopsis.Plant Cell516611668
- 28. Gendreau E, Traas J, Desnos T, Grandjean O, Caboche M, et al. (1997) Cellular basis of hypocotyl growth in Arabidopsis thaliana. Plant Phys 114: 295–305.E. GendreauJ. TraasT. DesnosO. GrandjeanM. Caboche1997Cellular basis of hypocotyl growth in Arabidopsis thaliana.Plant Phys114295305
- 29. Breuer C, Stacey NJ, Roberts G, West CE, Zhao Y, et al. (2007) Arabidopsis BIN4, a novel component of the plant DNA topoisomerase VI complex, promotes organ growth by endoreduplication. Plant Cell 19: 3655–3668.C. BreuerNJ StaceyG. RobertsCE WestY. Zhao2007Arabidopsis BIN4, a novel component of the plant DNA topoisomerase VI complex, promotes organ growth by endoreduplication.Plant Cell1936553668
- 30. Leiva-Neto JT, Grafi G, Sabelli PA, Dante RA, Woo Y-M, et al. (2004) A dominant negative mutant of Cyclin-Dependent Kinase A reduces endoreduplication but not cell size or gene expression in maize endosperm. Plant Cell 16: 1854–1869.JT Leiva-NetoG. GrafiPA SabelliRA DanteY-M Woo2004A dominant negative mutant of Cyclin-Dependent Kinase A reduces endoreduplication but not cell size or gene expression in maize endosperm.Plant Cell1618541869
- 31. Levan A (1939) Tetraploidy and octaploidy induced by colchicines in diploid Petunia. Hereditas 25: 109–131.A. Levan1939Tetraploidy and octaploidy induced by colchicines in diploid Petunia.Hereditas25109131
- 32. Smith HH (1939) The induction of polyploidy in Nicotiana species and species hybrids. J Hered 30: 291–306.HH Smith1939The induction of polyploidy in Nicotiana species and species hybrids.J Hered30291306
- 33. Simonet MMM, Chopinet R (1940) Sur l'obtention de plantes octoploïdes et hétéroploïdes après traitement par la colchicine. Revue Cytol Cytophysiol Végétales 4: 231–238.MMM SimonetR. Chopinet1940Sur l'obtention de plantes octoploïdes et hétéroploïdes après traitement par la colchicine.Revue Cytol Cytophysiol Végétales4231238
- 34. Fankhauser G (1952) Nucleo-cytoplasmic relations in amphibian development. Int Rev Cytol 1: 165–193.G. Fankhauser1952Nucleo-cytoplasmic relations in amphibian development.Int Rev Cytol1165193
- 35. Fankhauser G (1945) Maintenance of normal structure in heteroploid salamander larvae, through compensation of changes in cell size by adjustment of cell number and cell shape. J Exp Zool 100: 445–455.G. Fankhauser1945Maintenance of normal structure in heteroploid salamander larvae, through compensation of changes in cell size by adjustment of cell number and cell shape.J Exp Zool100445455
- 36. Fankhauser G (1938) The microscopical anatomy of metamorphosis in a haploid salamander, Triton taeniatus Lauer. J Morph 62: 393–413.G. Fankhauser1938The microscopical anatomy of metamorphosis in a haploid salamander, Triton taeniatus Lauer.J Morph62393413
- 37. Fankhauser G (1939) Polyploidy in the salamander, Eurycea bislineata. J Hered 30: 379–388.G. Fankhauser1939Polyploidy in the salamander, Eurycea bislineata.J Hered30379388
- 38. Henery CC, Bard JBL, Kaufman MH (1992) Tetraploidy in mice, embryonic cell number, and the grain of the developmental map. Dev Biol 152: 233–241.CC HeneryJBL BardMH Kaufman1992Tetraploidy in mice, embryonic cell number, and the grain of the developmental map.Dev Biol152233241
- 39. Comai L (2005) The advantages and disadvantages of being polyploid. Nature Genet 6: 836–846.L. Comai2005The advantages and disadvantages of being polyploid.Nature Genet6836846
- 40. Storchová Z, Breneman A, Cande J, Dunn J, Burbank K, et al. (2006) Genome-wide genetic analysis of polyploidy in yeast. Nature 443: 541–547.Z. StorchováA. BrenemanJ. CandeJ. DunnK. Burbank2006Genome-wide genetic analysis of polyploidy in yeast.Nature443541547