Calorie restriction (CR) robustly extends the lifespan of numerous species. In the yeast Saccharomyces cerevisiae, CR has been proposed to extend lifespan by boosting the activity of sirtuin deacetylases, thereby suppressing the formation of toxic repetitive ribosomal DNA (rDNA) circles. An alternative theory is that CR works by suppressing the TOR (target of rapamycin) signaling pathway, which extends lifespan via mechanisms that are unknown but thought to be independent of sirtuins. Here we show that TOR inhibition extends lifespan by the same mechanism as CR: by increasing Sir2p activity and stabilizing the rDNA locus. Further, we show that rDNA stabilization and lifespan extension by both CR and TOR signaling is due to the relocalization of the transcription factors Msn2p and Msn4p from the cytoplasm to the nucleus, where they increase expression of the nicotinamidase gene PNC1. These findings suggest that TOR and sirtuins may be part of the same longevity pathway in higher organisms, and that they may promote genomic stability during aging.
There are only a few techniques that reliably promote longevity in multiple, distantly related species. Perhaps the best known, caloric restriction (CR), was first shown to promote lifespan in rodents in the 1930s and has since been shown to work in most species it has been tested on. We and others have previously proposed that CR extends lifespan in budding yeast by boosting the activity of sirtuin deacetylases, which work to extend lifespan by suppressing genomic instability. A competing theory is that CR works by suppressing the TOR (target of rapamycin) signaling pathway, which has recently been discovered to extend the lifespan of yeast and worms, but the downstream players are not yet known. We show that TOR inhibition and sirtuins are part of the same CR pathway that extends yeast lifespan by stabilizing the genome. CR and TOR inhibition promote longevity by relocalizing two transcription factors, Msn2p and Msn4p, from the cytoplasm to the nucleus, where they increase expression of the nicotinamidase gene PNC1, a regulator of sirtuin activity. We propose that TOR signaling and sirtuins may also be part of the same CR pathway in mammals.
Citation: Medvedik O, Lamming DW, Kim KD, Sinclair DA (2007) MSN2 and MSN4 Link Calorie Restriction and TOR to Sirtuin-Mediated Lifespan Extension in Saccharomyces cerevisiae. PLoS Biol 5(10): e261. doi:10.1371/journal.pbio.0050261
Academic Editor: Andy Dillin, The Salk Institute, United States of America
Received: March 1, 2006; Accepted: August 6, 2006; Published: October 2, 2007
Copyright: © 2007 Medvedik et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: This work was supported by US National Institutes of Health National Institute on Aging grants RO1AG19972 and R01GM068072, the Harvard-Armenise Foundation, and the Paul F. Glenn Laboratories for the Biological Mechanisms of Aging. DWL is supported by a National Eye Institute training grant and is an Albert J. Ryan fellow.
Competing interests: DAS is a founder, director, share holder, and scientific consultant to Sirtris Pharmaceuticals, a company whose goal is to develop sirtuin-activating molecules to treat disease.
Abbreviations: CR, calorie restriction; ERC, extrachromosomal ribosomal DNA circle; NAM, nicotinamide; rDNA, ribosomal DNA; STRE, stress response element; YPD, yeast peptone dextrose
In the budding yeast Saccharomyces cerevisiae, replicative lifespan is measured by the number of divisions that a mother cell undergoes before senescing [1–3]. A primary cause of aging in this organism is homologous recombination between ribosomal DNA (rDNA) repeats, resulting in the formation of extrachromosomal rDNA circles (ERCs) that accumulate to toxic levels in mother cells . Sir2p and a closely related homolog, Hst2p, belong to the sirtuin family of NAD+-dependent deacetylases  that can forestall aging by stabilizing the rDNA locus [4,6,7]. Although rDNA recombination is not known to play a role in the aging of metazoans, the function of Sir2p enzymes in lifespan determination appears to be conserved. In Caenorhabditis elegans and Drosophila melanogaster, additional copies of the SIR2 gene or pharmacological modulation of the Sir2p deacetylase also extend lifespan [8–11].
The diet known as calorie restriction (CR) prolongs the lifespan of numerous species, including fungi, invertebrates, and mammals [1,12,13]. Whether or not Sir2p enzymes play a role in CR-mediated lifespan extension is hotly debated. In support of their playing a role, additional copies of either SIR2 or HST2 suppress rDNA recombination and extend yeast replicative lifespan, whereas strains lacking SIR2 and HST2 fail to live longer when subjected to CR [14,15]. Similarly, CR diets or genetic mimics of CR fail to extend the lifespan of D. melanogaster and C. elegans lacking Sir2p [12,16]. However, other researchers favor a model in which Sir2p plays no role in CR-mediated lifespan extension, and instead the TOR (target of rapamycin) pathway is proposed to play the central role [17,18].
TOR is a nutrient-responsive phosphatidylinositol-kinase-related kinase that regulates protein synthesis and cell growth, and is inhibited by rapamycin, an immunosuppressive and anticancer drug that specifically inhibits TOR . It has recently been discovered that lifespan can be extended in a variety of species by inhibition of TOR signaling, including S. cerevisiae, C. elegans, and D. melanogaster [17,20,21]. The mechanism by which inhibition of TOR signaling extends lifespan is unclear, but it has been proposed that it may act by altering ribosome assembly and translation [17,21–23]. Similar to CR, inhibition of TOR signaling can extend yeast lifespan in the absence of SIR2 , but whether SIR2 plays a role in lifespan extension during inhibition of TOR signaling is not known.
We have previously investigated the pathways by which CR operates in yeast. The enzymatic activity of Sir2p is regulated by endogenous levels of nicotinamide (NAM), a sirtuin inhibitor . Yeast strains grown on standard 2% glucose medium have an intracellular concentration of ∼50 μM NAM, which is almost precisely the IC50 of Sir2p [24–28]. We and others have shown that CR and other mild stresses, including heat stress and osmotic shock, extend yeast lifespan by increasing expression of the PNC1 gene, which encodes a nicotinamidase [27,29]. Recent evidence indicates that mammalian Nampt/PBEF, a putative functional ortholog of PNC1 that is required for the conversion of NAM to NAD+, also regulates sirtuin activity [30,31].
PNC1 is an intriguing longevity gene because its expression is regulated by environmental stimuli that extend lifespan, such as heat, osmotic stress, low amino acids, and CR. Binding sites for the stress-responsive zinc-finger transcription factors Msn2p and Msn4p have been identified in the PNC1 promoter . Msn2p and Msn4p have previously been shown to regulate chronological lifespan extension in response to deletion of the yeast Akt homolog SCH9 by controlling the expression of the superoxide dismutase SOD2 [33,34]. Msn2p/4p are therefore good candidates to regulate PNC1 expression in response to nutrient availability, but a previous study, which used a cdc25–10 mutant strain to mimic CR, concluded that MSN2 and MSN4 play no role in CR-mediated lifespan extension .
Here we show that Msn2p/4p are important regulators of yeast replicative lifespan and that they relocalize from the cytoplasm to the nucleus during CR, where they bind to and activate the PNC1 gene. Moreover, inhibition of TOR signaling acts via this same pathway to promote the expression of PNC1 and suppress rDNA recombination. These data provide evidence for a pathway from the cell's environment to an actual cause of aging, via which both CR and TOR signaling modulate lifespan.
We began by asking whether MSN2/4 are mediators of yeast lifespan extension by CR (0.5% glucose). In contrast to previous work using a genetic mimic of CR , we found that lifespan extension by CR was completely MSN2/4-dependent (Figure 1A). Single deletions of MSN2 or MSN4 did not block the ability of CR to extend lifespan, consistent with their known redundancy (Figure 1B). Interestingly, MSN2 and MSN4 were also absolutely necessary for lifespan extension resulting from TOR inhibition (Figure 1C).
(A) MSN2/4 are required for lifespan extension by CR (growth on 0.5% glucose). Average lifespans: W303 (wild-type [wt]) on 2% glucose, 25.0 divisions; W303 on CR diet, 30.8; msn2Δ/4Δ on 2% glucose, 24.2; and msn2Δ/4Δ on CR diet, 23.9.
(B) The lifespans of msn2Δ and msn4Δ single mutants can be extended by CR. Average lifespans: msn2Δ on 2% glucose, 22.5 divisions; msn2Δ on CR diet, 29.9; msn4Δ on 2% glucose, 24.7; and msn4Δ on CR diet, 33.2.
(C) MSN2/4 are required for lifespan extension by rapamycin (Rap). Average lifespans: wild-type, 23.3 divisions; wild-type + 1 nM rapamycin, 26.9; msn2Δ/4Δ, 23.8; and msn2Δ/4Δ + 1 nM rapamycin, 22.5.
The requirement of MSN2/4 for CR- and TOR-mediated lifespan extension indicated that CR and TOR might extend lifespan via the same mechanism. We and others have presented evidence that CR works by increasing Sir2p activity [8,14,29], as indicated by increases in telomeric silencing and a reduction in rDNA recombination [7,15,35]. We therefore examined whether rapamycin increased telomeric silencing and reduced rDNA recombination, and whether this was altered by the presence or absence of MSN2/4 or PNC1. We observed that rapamycin increased telomeric silencing in a wild-type strain, but not in strains lacking MSN2/4 or PNC1 (Figure S1). Treatment with rapamycin also suppressed rDNA recombination and, again, this effect required MSN2/4 and PNC1 (Figure 2A and 2B). The effect of rapamycin on silencing and rDNA recombination was also completely blocked by treating cells with NAM (Figure 2B), indicating that a sirtuin is likely required for this effect. Neither CR nor rapamycin increased protein levels of Sir2p (Figure 2C). Taken together, these data indicate that inhibition of TOR signaling increases the activity of Sir2p and/or another sirtuin.
(A) Rapamycin and CR suppress rDNA recombination in an MSN2/4-dependent manner.
(B) Deletion of PNC1 or treatment with NAM blocks the ability of CR or rapamycin treatment to lower rDNA recombination.
(C) CR and rapamycin treatment do not increase Sir2p levels.
(D) Deletion of PNC1 blocks the ability of rapamycin to extend lifespan. Average lifespans: wild-type (wt), 23.3 divisions; wild-type + 1 nM rapamycin, 26.9; pnc1Δ, 20.9; and pnc1Δ + 1 nM rapamycin, 21.4.
(E) Overexpression (o/e) of PNC1 suppresses rDNA recombination in both wild-type and msn2Δ/4Δ strains.
(F) Expression of PNC1 can extend the lifespan of a strain lacking MSN2/4. Average lifespans: wild-type, 23.2 divisions; wild-type + PNC1, 28.0; msn2Δ/4Δ, 22.0; and msn2Δ/4Δ + PNC1, 27.3.
Glu, glucose; Rap, rapamycin.
In addition to Sir2p, S. cerevisiae contains four HST (homolog of sir two) genes, HST1–4. We and others have uncovered considerable redundancy in the sirtuin family, with Hst1p and Hst2p able to substitute for Sir2p during CR . Recent studies in our laboratory have demonstrated that overexpression of any one of the sirtuin genes HST1–4 can suppress rDNA recombination (D. Lamming and M. Latorre-Esteves, unpublished data). Thus, we were not surprised to find that rapamycin could suppress rDNA recombination in a sir2Δ fob1Δ strain (Figure S2). This result supports a previously published report that inhibition of TOR signaling can extend lifespan in the absence of SIR2 . Although TOR inhibition can suppress rDNA recombination in the absence of SIR2, deletion of both SIR2 and HST2 blocked the ability of rapamycin to suppress rDNA recombination (Figure S2). Consistent with these data, we found that rapamycin could extend the lifespan of a sir2Δ fob1Δ strain but had no effect on the lifespan of a sir2Δ hst2Δ fob1Δ strain (Figure S2). Thus, rather than working through a single sirtuin, we favor a model in which inhibition of TOR signaling suppresses rDNA recombination and promotes longevity by activating multiple sirtuins, including Sir2p and Hst2p.
In agreement with this model, PNC1 was required for lifespan extension by rapamycin (Figure 2D), and overexpression of PNC1 was sufficient to suppress rDNA recombination and extend lifespan in an msn2Δ/4Δ background (Figure 2E and 2F). Thus, within the framework of our model, TOR signaling is upstream of PNC1 and PNC1 is downstream of MSN2/4. The simplest mechanistic explanation is that inhibition of TOR activates MSN2 and MSN4, which then increase the expression of PNC1. To test this, we examined the effect of CR and rapamycin on Pnc1p levels in the presence and absence of MSN2/4. Both treatments induced Pnc1p-GFP (Figure 3A) as well as native Pnc1p (Figure 3B). In contrast, there were considerably lower levels of Pnc1p in the untreated msn2Δ/4Δ strain, and Pnc1p levels remained below those of the untreated wild-type strain, even in response to CR or rapamycin (Figure 3B).
(A) Expression of Pnc1p-GFP is induced by CR and by rapamycin.
(B) Western blotting shows that CR and rapamycin induce high levels of Pnc1p in an MSN2/4-dependent manner.
(C) A cdc25–10 mutant has increased expression of PNC1 but not if MSN2/4 are deleted.
(D) Increased expression of PNC1 in response to salt stress, osmotic stress, and amino acid restriction requires MSN2/4, but increased expression of PNC1 in response to heat shock does not.
(E) MSN2/4 are not required for lifespan extension by heat shock. Average lifespans: wild-type at 30 °C, 22.2 divisions; wild-type at 37 °C, 26.8; msn2Δ/4Δ at 30 °C, 20.7; and msn2Δ/4Δ at 37 °C, 25.8.
(F) Heat shock induction of PNC1 in an msn2Δ/4Δ strain is dependent upon HSF1.
2/4Δ, msn2Δ/4Δ; dox, doxycycline; Glu, glucose; low a.a., low amino acids; rap, rapamycin; Sorb, sorbitol; wt, wild-type.
Msn2p/4p are normally maintained in the cytoplasm by the activity of PKA. As cAMP levels fall, PKA activity decreases, and dephosphorylated Msn2p/4p relocalize to the nucleus . A cdc25–10 mutant that has constitutively decreased cAMP/PKA signaling  expressed higher levels of Pnc1p than the wild-type strain, and this was MSN2/4-dependent (Figure 3C). Upregulation of PNC1 expression by other stresses was also MSN2/4-dependent (Figure 3D), and in agreement with the lifespan data in Figure 1B, single deletion of MSN2 or MSN4 did not greatly affect the ability of CR to induce PNC1 (Figure S3).
We have previously shown that heat shock extends lifespan in a PNC1-dependent manner , so we were curious whether this was an MSN2/4-dependent process. Heat shock induced the expression of PNC1 and extended lifespan even in the absence of MSN2/4 (Figure 3D and 3E). To explore the MSN2/4-independent mechanism by which heat shock induces PNC1, we analyzed the PNC1 promoter and found putative binding sites for the heat shock factor Hsf1p (positions −251 to −275 and −319 to −353, with respect to the start codon). Since HSF1 is an essential gene, we used an msn2Δ/4Δ strain containing doxycycline-repressible HSF1 to examine the role of Hsf1p in PNC1 regulation . Repression of HSF1 largely blocked the ability of heat shock to upregulate PNC1 (Figure 3F), but it did not affect the ability of CR or rapamycin to induce PNC1 (data not shown).
Under standard yeast growth conditions (2% glucose, 30 °C), Msn2p/4p localize predominately to the cytoplasm , but in response to a variety of stresses, Msn2p/4p localize to the nucleus, where they activate stress-responsive genes . We observed that CR induced the translocation of Msn2p into the nucleus, and the extent of the translocation was proportional to the degree of CR (Figure 4A and 4B). CR also induced the nuclear localization of Msn4p, but quantification of the nuclear localization indicated that Msn4p was less sensitive to glucose restriction than Msn2p (Figure S4). The reasons for this difference are unknown, but it may allow for differential gene regulation as nutrients are depleted. Nuclear localization of Msn2p also increased in both tor1Δ and cdc25–10 strains (Figure 4C and 4D), in agreement with previous reports [40,41].
(A) CR promotes increased nuclear migration of Msn2p-GFP in a dose-dependent manner.
(B) Quantification of the percent of cells with predominately nuclear Msn2p-GFP or Msn4p-GFP.
(C) Deletion of TOR1 promotes nuclear localization of Msn2p-GFP.
(D) A cdc25–10 mutant has increased nuclear localization of Msn2p-GFP.
Cells grown in moderate CR conditions (0.5% and 0.1% glucose) showed heterogeneous localization patterns for Msn2p/4p, indicating that Msn2p/4p might be oscillating between the nucleus and cytoplasm, as has recently been noted for cells exposed to light stress or osmotic shock . To determine if this was the case, time-lapse photomicrographs of cells expressing Msn2p-GFP were taken at 30-s intervals during growth under various glucose concentrations. The vast majority of cells grown in 2% glucose showed a cytoplasmic localization of Msn2p (Figure 5A), while cells incubated in medium lacking glucose had exclusively nuclear localization of Msn2p (Figure 5B). These patterns did not change over time. However, in cells grown under intermediate levels of CR (0.1% or 0.5% glucose), nucleo-cytoplasmic oscillations of Msn2p-GFP were observed (Figures 5C and S5). The GFP signal eventually became bleached (data not shown), demonstrating that Msn2p was not simply being degraded in the nucleus and re-synthesized in the cytoplasm but was instead actively being transported in and out of the nucleus . Msn4p-GFP showed a similar oscillatory behavior, and localization of Msn2p or Msn4p under 2% or 0.5% glucose was not altered by a lack of the other transcription factor (data not shown).
During CR, Msn2p-GFP oscillates between the cytoplasm and nucleus.
(A and B) Cells grown at 2% glucose concentrations (A) exhibit exclusively cytoplasmic localization of Msn2p-GFP. Cells placed in medium containing 0% glucose (B) exhibit exclusively nuclear localization of Msn2p-GFP. For cells in 2% glucose or 0% glucose, a plot of the ratio of average nuclear intensity versus average cytoplasmic intensity for a single representative cell, labeled with an arrowhead in the respective photomicrographs, is shown.
(C) During CR conditions, cells exhibit nucleo-cytoplasmic oscillations of Msn2p-GFP with a periodicity of ∼2–3 min. A representative population of cells is shown at 0.1% glucose concentrations. The ratios of average nuclear intensity versus average cytoplasmic intensity for three representative cells, labeled a, b, and c, are shown plotted over the course of 20 min beneath the photomicrographs.
The PNC1 promoter contains four Msn2p/4p-binding sites known as stress response elements (STREs) . To determine whether Msn2p/4p directly regulate PNC1 in response to CR, we tested several reporter constructs containing different regions of the PNC1 promoter (Figure 6A). The full-length promoter construct was greatly induced in response to CR (Figure 6B), whereas the reporters lacking one or more of the STREs were induced at significantly lower levels. The construct with no STREs showed no induction. The involvement of MSN2/4 was confirmed by the finding that CR completely failed to upregulate the reporter in an msn2Δ/4Δ strain (Figure 6C). The ability of CR to induce the PNC1 reporter was largely unaffected in single msn2Δ or msn4Δ mutants (Figure 6D and 6E), which is consistent with the ability of CR to extend the lifespan of single but not double msn mutants (see Figure 1A and 1B). Interestingly, deletion of MSN4 did not affect the expression of the STRE4 reporter construct under 2% glucose, but significantly reduced its response to CR (Figure 6E). In contrast, the STRE2 reporter construct was unaffected by the MSN4 deletion, indicating that the influence of Msn4p on PNC1 expression may be mediated by the distal two STRE elements.
(A) Reporter constructs for analysis of STRE and Msn2p/4p function.
(B) β-galactosidase activity of reporter constructs in wild-type (wt) cells grown in 2% YPD (Glu) (white bars), 0.5% YPD (black bars), or 2%YPD at 37 °C (grey bars).
(C–E) Activity of reporter constructs in (C) an msn2Δ/4Δ strain, (D) an msn2Δ strain, or (E) an msn4Δ strain. Activity is listed as nanomoles of cleaved o-nitrophenol-β-D-galactopyranoside substrate per minute per milligram total protein.
We have previously shown that while single deletion of MSN2 impairs the ability of PNC1 expression to be induced by stress, single deletion of MSN4 has little effect (Figure S3). Furthermore, Msn2p localizes to the nucleus more readily in response to decreased glucose concentration (compare Figures 4 and S4), deletion of MSN2 results in approximately 2-fold less expression of the reporter construct than deletion of MSN4, and the STRE2 constructs were less responsive to CR in an msn2Δ strain than in an msn4Δ strain (Figure 6D and 6E). Together these data support the conclusion that Msn2p is more important than Msn4p for regulating PNC1 expression in response to CR.
Msn2p also appears to be more important for the response of PNC1 to heat stress (37 °C). Deletion of MSN2 resulted in an approximately 2-fold decrease in the expression of the STRE4 reporter construct in response to heat, relative to the wild-type strain (compare Figure 6B and 6D). In contrast, single deletion of MSN4 actually leads to increased expression of the STRE 4 reporter in response to heat (compare Figure 6B and 6E). This upregulation in the msn4Δ strain is likely to be due to MSN2, because deletion of MSN2 in the msn4Δ strain resulted in the poorest response of the STRE4 reporter to heat (Figure 6C). The data also indicated that elements present in the STRE4 reporter but not in the STRE2 reporter are responsible for the MSN2/4-independent induction of the promoter in response to heat stress. Consistent with this hypothesis, the putative binding sites for Hsf1p identified by our promoter analysis lie within this region.
Given the relative importance of Msn2p, we next asked if the nuclear localization of Msn2p was sufficient to induce PNC1. We expressed a constitutively nuclear mutant of Msn2p, Msn2p(S4A) , in an msn2Δ and msn4Δ mutant, and observed robust induction of Pnc1p (Figure 7A), demonstrating that nuclear localization of Msn2p is sufficient to induce PNC1 expression. We also noted that this strain grew poorly, in agreement with previous reports that constitutive nuclear localization of Msn2p/4p antagonizes growth .
(A) Nuclear localization of Msn2p is sufficient to induce PNC1.
(B) Chromatin immunoprecipitation analysis of Msn2p-HA binding to the PNC1 promoter. IN, input; IP, immunoprecipitation.
(C) Relative enrichment of Msn2p-HA at the PNC1 promoter shows increased promoter binding during CR.
The data thus far strongly supported a model in which CR promotes the binding of Msn2p directly to the PNC1 promoter, yet there remained the possibility that PNC1 was regulated by Msn2p indirectly. To distinguish between these two possibilities, we used chromatin immunoprecipitation to determine if Msn2p binds to the PNC1 promoter in response to CR (Figure 7B). We detected Msn2p at the PNC1 promoter, and the apparent abundance of Msn2p was proportional to the degree of CR (Figure 7C), which was consistent with the glucose-dependent nuclear localization of Msn2p that we previously observed.
In order to better understand the target specificity of Msn2p/4p in response to CR, we utilized previously published microarray data [45,46] to examine the expression of 82 previously identified STRE-containing genes . We were surprised to find that the genes varied greatly in their responsiveness to conditions of low glucose. Ten genes, including PNC1, were highly responsive to small changes in glucose concentration, whereas other STRE-containing genes were responsive only to large changes in glucose concentration or were unresponsive (Table S1). In an effort to understand why some genes are more responsive to Msn2p/4p than others, we analyzed the promoters of these STRE-containing genes. The genes that were less responsive to low glucose averaged fewer STRE elements than the highly responsive genes and, on average, had binding sites approximately 50–60 base pairs further from the start of the coding sequence than genes that responded to low glucose (Table S2). We speculate that the placement of transcription factor binding sites at varying distances from the promoter may be a conserved mechanism for the differential regulation of stress-induced genes.
Because our work indicated that two major longevity pathways, namely CR and TOR, promote lifespan by inducing the expression of PNC1, we wondered whether other yeast longevity genes also modulate PNC1 expression. Deletion of the glycolysis pathway gene HXK2 extends lifespan and has been proposed as a genetic mimic of CR [15,17,37]. Consistent with this, an hxk2Δ strain had higher levels of PNC1 (Figure S6), placing it upstream of PNC1. Similarly, there are abundant data linking the Snf1p/AMPK pathway to yeast longevity. The yeast homolog of AMPK, Snf1p, phosphorylates Msn2p in response to glucose deprivation, is regulated by TOR, and influences lifespan by modulating ERC formation [48,49]. Deletion of SNF4, an activator of Snf1p, has been shown to extend lifespan, whereas deletion of SIP2, a repressor of Snf1p, shortens lifespan . Surprisingly, we saw no evidence for involvement of the SNF pathway (SNF1, SNF4, or SIP2) in the regulation of PNC1 (Figure S6), indicating that the activity of Snf1p/AMPK regulates ERC formation independently of PNC1. Furthermore, CR induced PNC1 expression equally well in wild-type and snf1Δ mutant strains (Figure S6).
We also examined the potential role of ADR1, a transcription factor that is regulated by SNF1/AMPK and that we suspected from our promoter analysis of PNC1 might regulate PNC1 . We found that ADR1 was not required for the induction of PNC1 by CR (Figure S6 and data not shown), and that overexpression of ADR1, or expression of a constitutively active form of Adr1p, did not induce expression of PNC1 (Figure S6). Together, these data show that while attenuation of TOR signaling, PKA activity (cdc25–10), or glucose metabolism (hxk2Δ) extends replicative lifespan, ostensibly by mimicking the effects of CR, the SNF pathway regulates lifespan via a PNC1-independent mechanism.
How CR delays aging and extends the lifespan of various species is poorly understood. In this study, we have connected two sections of the yeast CR pathway, namely the cytoplasmic components (the glucokinase/cAMP/PKA pathway) and the nuclear components (Pnc1p, Sir2p, and ERCs). Furthermore, we have shown that TOR signaling, which was previously thought to regulate lifespan independently of sirtuins and ERCs, actually governs the activity of the sirtuins and suppresses rDNA recombination (Figure 8). This provides additional support to the theory that CR extends replicative lifespan, at least in part, by activating sirtuins.
CR and TOR signaling regulate the nuclear localization of Msn2p/4p. When localized to the nucleus, Msn2p/4p promote the transcription of PNC1, a nicotinamidase. The removal of NAM by Pnc1p increases the activity of sirtuins, including Sir2p and Hst2p, which promote longevity by stabilizing the yeast rDNA array and preventing the formation of ERCs. TOR signaling may also regulate lifespan by sirtuin-independent pathways, such as the regulation of ribosomal biogenesis.
We also demonstrate that the induction of PNC1 in response to numerous stresses is largely controlled by the transcription factors Msn2p and Msn4p. Under conditions of high salt or sorbitol, PNC1 expression is increased in a manner that is completely dependent on MSN2/4 (Figure 3C). While we have linked the increase in Pnc1p levels during heat stress in an msn2Δ/4Δ strain to the transcription factor Hsf1p (Figure 3F), we did not observe a role for Hsf1p in the response to CR or low amino acids.
There must be additional factors that control the expression of PNC1, because an increase in Pnc1p levels still occurs in an msn2Δ/4Δ strain grown in 0.5% glucose or in medium with low amino acids. One possibility is that PNC1 is co-activated by Gcr1p, a transcriptional activator with potential binding sites ∼500 base pairs upstream of the PNC1 start codon. GCR1 regulates glycolytic enzyme genes, ribosomal gene synthesis, and trehalose/glycogen metabolism [51,52], making it an interesting candidate for future analysis, although we note that any such analysis is complicated by the severe growth defect of a gcr1Δ strain.
In contrast to our study, a previous study utilizing a cdc25–10 mutant as a mimic of CR found that replicative lifespan extension of a PSY316 strain can occur in the absence of MSN2/4 . A recent study has shown that PSY316 may differ substantially from other yeast strains in terms of Sir2p-mediated lifespan extension , and our data may reflect that difference. We favor the notion that while the cdc25–10 mutation mirrors aspects of CR, such as lower PKA activity and increased lifespan, it does not fully replicate it.
A previous study has shown that inhibition of TOR signaling can extend lifespan, even in the absence of SIR2 . In agreement with this data, we find that treatment with rapamycin can suppress rDNA recombination and extend lifespan in a sir2Δ fob1Δ strain (Figure S2). Yeast contain four additional sirtuin genes (HST1–4), some of which can compensate for the lack of Sir2p during CR . Under the conditions and with the strain used in this study, we have observed that rapamycin no longer lowers rDNA recombination or promotes longevity if both SIR2 and HST2 are deleted (Figure S2), implying that these two genes are primarily responsible for the effect. However, a W303 sir2Δ hst2Δ fob1Δ strain has a high rate of rDNA recombination and a short lifespan, which may serve to obscure the role of additional sirtuins or other mediators in the response to TOR inhibition. In fact, overexpression of PNC1 in a wild-type strain lowers rDNA recombination more than in a strain lacking MSN2/4, which may indicate that genes downstream of MSN2/4 besides PNC1 also function to repress rDNA recombination. These alternative pathways may be especially important when glucose concentrations are extremely low  and may include pathways that directly regulate rDNA stability, such as RPD3-dependent loading of condensin onto the rDNA array in response to nutrient signaling . TOR signaling also promotes the synthesis of ribosomal proteins, and downregulation of ribosomal biogenesis can extend the lifespan of both yeast  and C. elegans [22,23]. These data suggest that TOR signaling may act to promote lifespan via multiple pathways that act in parallel to promote longevity (Figure 8).
Our analysis of the responsiveness of STRE-containing genes found ten genes, including PNC1, that are upregulated more than 2-fold in response to a slight decrease in the glucose concentration (2% to 1.75%) (Table S1). In general, the genes in this category are highly sensitive to environmental stresses, including heat shock and osmotic stress . We speculate that other genes in this category, which includes both metabolic and heat shock genes, may also play a role in lifespan extension. Heat shock proteins in particular have been shown to promote longevity in numerous organisms, and are upregulated during CR in rodents [55,56].
Interestingly, MSN2/4 have also been shown to be required for the extension of yeast chronological lifespan . MSN2/4 are responsible for the activation of numerous stress-responsive genes, including the superoxide dismutase SOD2, a gene that promotes chronological lifespan . Yet, overexpression of SOD2 shortens replicative lifespan, and it has been demonstrated that deletion of MSN2/4 can actually lead to increases in replicative lifespan . Even though we saw no such effect (Figure 1A), perhaps because of a difference in strain background, there may be a reciprocal relationship between replicative and chronological lifespan. A recent study showed that deletion of SIR2 can extend chronological lifespan in several strains , and we have observed that overexpression of SIR2 or HST2 shortens chronological lifespan in W303 (unpublished data).
The identification of the stress response factors Msn2p/4p as key components of the CR pathway in yeast supports two theories about CR. The first is known as the hormesis hypothesis of CR, which states that CR is a mild biological stress that provides health benefits because it activates an organism's defenses against adversity [60,61]. The second hypothesis is that the promoter elements of key longevity genes are just as important as the longevity genes themselves [28,62]. These promoters serve as sensors of the organism's environment by accepting different and additive inputs from environmentally sensitive transcription factors. The existence of short DNA sequences that dictate longevity could explain how new lifespans evolve so rapidly in response to a new ecological niche. Theoretically, if a transcription factor binding site regulates a key longevity gene, then a single base change might be sufficient to alter how long a species lives in response to an environmental condition.
In contrast to previous suggestions, we find that TOR and sirtuin signaling are components of the same longevity pathway that extends yeast replicative lifespan by stabilizing the repetitive rDNA (Figure 8). Given the high degree of functional conservation of TOR and sirtuins between yeast and higher organisms, and the recent discovery of a role for mammalian sirtuins in DNA repair , the findings in this study raise the possibility that the mammalian TOR pathway influences sirtuin activity and that together they may promote the health and longevity of mammals.
Materials and Methods
Yeast strains and plasmids.
W303AR MATa, W303AR MATa pnc1::kanr, W303AR PNC1-GFP::kanr, and W303AR SIR2-3xHA::kanr have been previously described [24,29,64]. Gene disruptions in W303AR MATa were achieved by replacing the wild-type genes with the kanr, hphr, or natr marker as described [65,66] and verified by PCR using oligonucleotides flanking the genes. PNC1 was overexpressed as previously described . W303AR cdc25–10 was created by replacing the endogenous copy of CDC25 with a plasmid-borne copy of cdc25–10 (the kind gift of S. J. Lin) as previously described . pPNC1-STRE.4, pPNC1-STRE.2, and pPNC1-STRE.0 constructs and the pAdh-Msn2p-GFP/HA constructs were kindly provided by M. Ghislain  and C. Schuller , respectively. Msn4p-GFP and Msn2p(S4A)-GFP constructs were the kind gift of M. Jacquet. Plasmids for expression of Adr1p were obtained from E. Young. BY4741 deletions in this background were from F. Winston and P. Silver (Harvard), BY4742 and BY4742 hxk2::kanr were from B. Kennedy , W303 msn2Δ/4Δ and W303 msn2Δ/4Δ tetO-HSF1 were from H. Nelson . All primer sequences, strains, and plasmid maps are available upon request.
Yeast were grown in yeast peptone dextrose (YPD) medium supplemented with an additional 0.015% w/v adenine, histidine, leucine, tryptophan, and uridine, and containing 2% w/v glucose during normal growth and 0.5% glucose for CR unless otherwise stated. For growth in low amino acid medium, synthetic complete medium containing 0.03 % w/v essential amino acids and 2% glucose was used. Strains were pre-grown overnight at 30 °C. The following day, cells were inoculated at an O.D. 600 = 0.1 and grown until log phase of growth was attained during the various conditions mentioned (O.D. 600 = 0.7). For treatment with rapamycin or heat shock, cells were grown for 2 h untreated, at which point rapamycin was added to a final concentration of 1 nM, or cells were moved to 37 °C, and cells were then grown for an additional 2 h.
Recombination and lifespan assays.
rDNA recombination rates were determined by determining the frequency of loss of ADE2 in the rDNA of strain W303AR as previously described [15,29]. For rapamycin recombination assays, cells were grown for 2 h without rapamycin, followed by growth with 1 nM rapamycin for 2 h. More than 6,000 colonies were examined for each strain. Results are average values and standard deviation of at least three experiments. For replicative lifespan analyses, strains were pre-grown overnight on YPD plates unless otherwise noted. All lifespan analyses were carried out by using micromanipulation as previously described , and all micromanipulation dissections, including for cells grown under heat stress (37 °C), were carried out at laboratory temperature. For cells treated with rapamycin, yeast that growth-arrested in the G1 phase of the cell cycle due to toxicity  within the first nine divisions were excluded from the datasets. Statistical analysis was carried out using the JMP-IN statistics package (SAS, http://www.sas.com/). Wilcoxon rank-sum test p-values were calculated for each pair of lifespans, as shown in Table S3.
Fluorescence microscopy and imaging.
For the observation of nuclear migration of Msn2p-GFP or Msn4p-GFP, yeast were grown in YPD for 30 min at 1%, 0.5%, 0.1%, and 0.05% glucose (w/v), after first pre-growing to log phase in 2% glucose. Nuclei in live cells were stained with Hoechst #33342 (Sigma-Aldrich, http://www.sigmaaldrich.com/). Time-lapse photomicrographs were captured every 30 s using 1-s exposures. Image analysis was performed using the imageJ software package (National Institutes of Health, http://www.nih.gov/) in order to calculate the ratios of average nuclear intensity versus average cytoplasmic intensity.
β-galactosidase reporter assays and protein extraction.
Whole cell extracts were used to assay β-galactosidase activity as described . Enzymatic activity is expressed as nanomoles o-nitrophenol-β-D-galactopyranoside cleaved per minute per milligram total protein.
Western analysis and antibodies.
Rabbit anti-Pnc1p polyclonal antibodies were generated by immunization of rabbits (Covance, http://store.crpinc.com/) with recombinant protein, and fresh serum was used at a dilution of 1:5,000. Mouse monoclonal anti-β-tubulin antibody (MAB3408, clone KMX-1, Upstate, http://www.upstate.com/), mouse monoclonal anti-actin antibody (Upstate/Chemicon MAB1501) and polyclonal rabbit anti-HA antibody (Abcam, http://www.abcam.com/) were used at a dilution of 1:1,000. Anti-rabbit (Amersham, http://www.amersham.com/) and anti-mouse (Amersham) horse radish peroxidase–conjugated antibodies were used at dilutions of 1:7,000.
Chromatin immunoprecipitation assay and PCR analysis.
The chromatin immunoprecipitation procedure was a modification of the method described by Strahl-Bolsinger et al. . Changes to the protocol are as follows: 100 ml of cells (2.0 × 107 cells/ml) was cross-linked with 2% formaldehyde for 15 min at room temperature. Glycine was added to a final concentration of 250 mM, and the incubation continued for an additional 5 min. The suspension was sonicated seven times for 10 s with the amplitude set at 30% using a Branson model 450 digital sonifier (Branson, http://www.bransonultrasonics.com/). The suspension was clarified by centrifugation for 5 min, maximum setting, at 4 °C in a microcentrifuge. Samples were incubated on ice for 2 min between pulses. Then 1 μl of RNase (10 μg/ul) was added to samples, and they incubated for 30 min at 37 °C. Afterwards, sheared chromatin was purified using QIAquick spin columns (Qiagen, http://www1.qiagen.com/). Then 250 μl of supernatant was incubated with 15 μl of rabbit anti-HA antibody (Abcam). For the PCR analysis, the actin control primers used were as follows: ACT1-Chip-Fwd, GCCTTCTACGTTTCCATCCA , and ACT1-Chip-Rev, GGCCAAATCGATTCTC AAAA . The PNC1 promoter primers used were as follows: Pnc1p-Chip-Fwd, GATCAAGGTGGCACACAGGG, and Pnc1p-Chip-Rev, ATACATAGTGGGCCAAACGG. The PCR protocol used was one cycle with 2 min at 95 °C, 30 s annealing at 55 °C, and a 1-min extension at 72 °C, followed by 30 cycles with 30 s at 95 °C, 30 s annealing at 55 °C, and 1-min extension at 72 °C. A final extension was performed for 4 min at 72 °C. Specific binding of Msn2p-HA to the endogenous PNC1 promoter (PNC1p) was analyzed by calculating the ratio of the percent IP of PNC1p to the percent IP of ACT1, using the V4.2.2 Quantity One 1-D analysis package (Bio-Rad, http://www.bio-rad.com/).
Figure S1. Inhibition of TOR Signaling by 1 nM Rapamycin Increases Telomeric Silencing
(433 KB PDF)
Figure S2. Suppression of rDNA Recombination by Rapamycin Does Not Depend upon a Single Sirtuin
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Figure S3. Effect of Single Deletions of MSN2 or MSN4 on PNC1 Expression during Stresses
(7.3 MB PDF)
Figure S4. Msn4p Localizes to the Nucleus during CR
(1.2 MB PDF)
Figure S5. CR Induces Nucleo-Cytoplasmic Oscillations of Msn2p-GFP
(509 KB PDF)
Figure S6. Snf1p Signaling Does Not Regulate PNC1
(509 KB PDF)
Table S1. Expression of STRE-Containing Genes during the Diauxic Shift
(757 KB PDF)
Table S2. STRE Promoter Analysis
(12 KB PDF)
Table S3. Lifespan Analysis
Wilcoxon rank-sum test p-values were calculated for each pair of lifespans using JMP-IN statistical analysis software.
(23 KB PDF)
We thank members of the Sinclair laboratory for insights, and especially thank S. Armour, J. Baur, A. Hafner, and B. North for technical assistance and critical reading of the manuscript. We would also like to thank the reviewers for their helpful comments and suggestions. We thank M. Ghislain, M. Jacquet, S.-J. Lin, C. Schuller, and E. Young for gifts of plasmids, and B. Kennedy, H. Nelson, P. Silver, F. Winston, and M. Yu for strains.
OM, DWL, and DAS conceived and designed the experiments. OM, DWL, and KDK performed the experiments. All authors analyzed the data. OM, DWL, and DAS contributed reagents/materials/analysis tools and wrote the paper.
- 1. Jiang JC, Jaruga E, Repnevskaya MV, Jazwinski SM (2000) An intervention resembling caloric restriction prolongs life span and retards aging in yeast. FASEB J 14: 2135–2137.
- 2. Barton A (1950) Some aspects of cell division in Saccharomyces cerevisiae. J Gen Microbiol 4: 84–86.
- 3. Sinclair DA, Mills K, Guarente L (1998) Molecular mechanisms of yeast aging. Trends Biochem Sci 23: 131–134.
- 4. Sinclair DA, Guarente L (1997) Extrachromosomal rDNA circles—A cause of aging in yeast. Cell 91: 1033–1042.
- 5. Guarente L (2000) Sir2 links chromatin silencing, metabolism, and aging. Genes Dev 14: 1021–1026.
- 6. Kaeberlein M, McVey M, Guarente L (1999) The SIR2/3/4 complex and SIR2 alone promote longevity in Saccharomyces cerevisiae by two different mechanisms. Genes Dev 13: 2570–2580.
- 7. Fritze CE, Verschueren K, Strich R, Easton Esposito R (1997) Direct evidence for SIR2 modulation of chromatin structure in yeast rDNA. EMBO J 16: 6495–6509.
- 8. Howitz KT, Bitterman KJ, Cohen HY, Lamming DW, Lavu S, et al. (2003) Small molecule activators of sirtuins extend Saccharomyces cerevisiae lifespan. Nature 425: 191–196.
- 9. Tissenbaum HA, Guarente L (2001) Increased dosage of a sir-2 gene extends lifespan in Caenorhabditis elegans. Nature 410: 227–230.
- 10. Wood JG, Rogina B, Lavu S, Howitz K, Helfand SL, et al. (2004) Sirtuin activators mimic caloric restriction and delay ageing in metazoans. Nature 430: 686–689.
- 11. Viswanathan M, Kim SK, Berdichevsky A, Guarente L (2005) A role for SIR-2.1 regulation of ER stress response genes in determining C. elegans life span. Dev Cell 9: 605–615.
- 12. Rogina B, Helfand SL (2004) Sir2 mediates longevity in the fly through a pathway related to calorie restriction. Proc Natl Acad Sci U S A 101: 15998–16003.
- 13. McCay CM, Maynard LA, Sperling G, Barnes LL (1975) The Journal of Nutrition. Volume 18 July–December, 1939. Pages 1–13. Retarded growth, life span, ultimate body size and age changes in the albino rat after feeding diets restricted in calories. Nutr Rev 33: 241–243.
- 14. Lin SJ, Defossez PA, Guarente L (2000) Requirement of NAD and SIR2 for life-span extension by calorie restriction in Saccharomyces cerevisiae. Science 289: 2126–2128.
- 15. Lamming DW, Latorre-Esteves M, Medvedik O, Wong SN, Tsang FA, et al. (2005) HST2 mediates SIR2-independent life-span extension by calorie restriction. Science 309: 1861–1864.
- 16. Wang Y, Tissenbaum HA (2006) Overlapping and distinct functions for a Caenorhabditis elegans SIR2 and DAF-16/FOXO. Mech Ageing Dev 127: 48–56.
- 17. Kaeberlein M, Powers RW 3rd, Steffen KK, Westman EA, Hu D, et al. (2005) Regulation of yeast replicative life span by TOR and Sch9 in response to nutrients. Science 310: 1193–1196.
- 18. Kaeberlein M, Kirkland KT, Fields S, Kennedy BK (2004) Sir2-independent life span extension by calorie restriction in yeast. PLoS Biol 2: e296. doi:10.1371/journal.pbio.0020296.
- 19. Wullschleger S, Loewith R, Hall MN (2006) TOR signaling in growth and metabolism. Cell 124: 471–484.
- 20. Vellai T, Takacs-Vellai K, Zhang Y, Kovacs AL, Orosz L, et al. (2003) Genetics: Influence of TOR kinase on lifespan in C. elegans. Nature 426: 620.
- 21. Kapahi P, Zid BM, Harper T, Koslover D, Sapin V, et al. (2004) Regulation of lifespan in Drosophila by modulation of genes in the TOR signaling pathway. Curr Biol 14: 885–890.
- 22. Syntichaki P, Troulinaki K, Tavernarakis N (2007) eIF4E function in somatic cells modulates ageing in Caenorhabditis elegans. Nature 445: 922–926.
- 23. Hansen M, Taubert S, Crawford D, Libina N, Lee SJ, et al. (2007) Lifespan extension by conditions that inhibit translation in Caenorhabditis elegans. Aging Cell 6: 95–110.
- 24. Bitterman KJ, Anderson RM, Cohen HY, Latorre-Esteves M, Sinclair DA (2002) Inhibition of silencing and accelerated aging by nicotinamide, a putative negative regulator of yeast sir2 and human SIRT1. J Biol Chem 277: 45099–45107.
- 25. Sauve AA, Moir RD, Schramm VL, Willis IM (2005) Chemical activation of Sir2-dependent silencing by relief of nicotinamide inhibition. Mol Cell 17: 595–601.
- 26. Jackson MD, Schmidt MT, Oppenheimer NJ, Denu JM (2003) Mechanism of nicotinamide inhibition and transglycosidation by Sir2 histone/protein deacetylases. J Biol Chem 278: 50985–50998.
- 27. Gallo CM, Smith DL Jr, Smith JS (2004) Nicotinamide clearance by Pnc1 directly regulates Sir2-mediated silencing and longevity. Mol Cell Biol 24: 1301–1312.
- 28. Anderson RM, Bitterman KJ, Wood JG, Medvedik O, Sinclair DA (2003) Nicotinamide and PNC1 govern lifespan extension by calorie restriction in Saccharomyces cerevisiae. Nature 423: 181–185.
- 29. Anderson RM, Bitterman KJ, Wood JG, Medvedik O, Cohen H, et al. (2002) Manipulation of a nuclear NAD+ salvage pathway delays aging without altering steady-state NAD+ levels. J Biol Chem 277: 18881–18890.
- 30. van der Veer E, Ho C, O'Neil C, Barbosa N, Scott R, et al. (2007) Extension of human cell lifespan by nicotinamide phosphoribosyltransferase. J Biol Chem 282: 10841–10845.
- 31. Revollo JR, Grimm AA, Imai S (2004) The NAD biosynthesis pathway mediated by nicotinamide phosphoribosyltransferase regulates Sir2 activity in mammalian cells. J Biol Chem 279: 50754–50763.
- 32. Ghislain M, Talla E, Francois JM (2002) Identification and functional analysis of the Saccharomyces cerevisiae nicotinamidase gene, PNC1. Yeast 19: 215–224.
- 33. Fabrizio P, Pozza F, Pletcher SD, Gendron CM, Longo VD (2001) Regulation of longevity and stress resistance by Sch9 in yeast. Science 292: 288–290.
- 34. Fabrizio P, Liou LL, Moy VN, Diaspro A, SelverstoneValentine J, et al. (2003) SOD2 functions downstream of Sch9 to extend longevity in yeast. Genetics 163: 35–46.
- 35. Aparicio OM, Billington BL, Gottschling DE (1991) Modifiers of position effect are shared between telomeric and silent mating-type loci in S. cerevisiae. Cell 66: 1279–1287.
- 36. Gorner W, Durchschlag E, Martinez-Pastor MT, Estruch F, Ammerer G, et al. (1998) Nuclear localization of the C2H2 zinc finger protein Msn2p is regulated by stress and protein kinase A activity. Genes Dev 12: 586–597.
- 37. Lin SJ, Kaeberlein M, Andalis AA, Sturtz LA, Defossez PA, et al. (2002) Calorie restriction extends Saccharomyces cerevisiae lifespan by increasing respiration. Nature 418: 344–348.
- 38. Ferguson SB, Anderson ES, Harshaw RB, Thate T, Craig NL, et al. (2005) Protein kinase A regulates constitutive expression of small heat-shock genes in an Msn2/4p-independent and Hsf1p-dependent manner in Saccharomyces cerevisiae. Genetics 169: 1203–1214.
- 39. Gorner W, Durchschlag E, Wolf J, Brown EL, Ammerer G, et al. (2002) Acute glucose starvation activates the nuclear localization signal of a stress-specific yeast transcription factor. EMBO J 21: 135–144.
- 40. Powers RW 3rd, Kaeberlein M, Caldwell SD, Kennedy BK, Fields S (2006) Extension of chronological life span in yeast by decreased TOR pathway signaling. Genes Dev 20: 174–184.
- 41. Beck T, Hall MN (1999) The TOR signalling pathway controls nuclear localization of nutrient-regulated transcription factors. Nature 402: 689–692.
- 42. Jacquet M, Renault G, Lallet S, De Mey J, Goldbeter A (2003) Oscillatory nucleocytoplasmic shuttling of the general stress response transcriptional activators Msn2 and Msn4 in Saccharomyces cerevisiae. J Cell Biol 161: 497–505.
- 43. Enninga J, Levay A, Fontoura BM (2003) Sec13 shuttles between the nucleus and the cytoplasm and stably interacts with Nup96 at the nuclear pore complex. Mol Cell Biol 23: 7271–7284.
- 44. Smith A, Ward MP, Garrett S (1998) Yeast PKA represses Msn2p/Msn4p-dependent gene expression to regulate growth, stress response and glycogen accumulation. EMBO J 17: 3556–3564.
- 45. DeRisi JL, Iyer VR, Brown PO (1997) Exploring the metabolic and genetic control of gene expression on a genomic scale. Science 278: 680–686.
- 46. Gasch AP, Spellman PT, Kao CM, Carmel-Harel O, Eisen MB, et al. (2000) Genomic expression programs in the response of yeast cells to environmental changes. Mol Biol Cell 11: 4241–4257.
- 47. Moskvina ESC, Maurer CT, Mager WH, Ruis H (1998) A search in the genome of Saccharomyces cerevisiae for genes regulated via stress response elements. Yeast 14: 1041–1050.
- 48. Orlova M, Kanter E, Krakovich D, Kuchin S (2006) Nitrogen availability and TOR regulate the Snf1 protein kinase in Saccharomyces cerevisiae. Eukaryot Cell 5: 1831–1837.
- 49. Ashrafi K, Lin SS, Manchester JK, Gordon JI (2000) Sip2p and its partner snf1p kinase affect aging in S. cerevisiae. Genes Dev 14: 1872–1885.
- 50. Young ET, Kacherovsky N, Van Riper K (2002) Snf1 protein kinase regulates Adr1 binding to chromatin but not transcription activation. J Biol Chem 277: 38095–38103.
- 51. Seker T, Hamamci H (2003) Trehalose, glycogen and ethanol metabolism in the gcr1 mutant of Saccharomyces cerevisiae. Folia Microbiol (Praha) 48: 193–198.
- 52. Deminoff SJ, Santangelo GM (2001) Rap1p requires Gcr1p and Gcr2p homodimers to activate ribosomal protein and glycolytic genes, respectively. Genetics 158: 133–143.
- 53. Easlon E, Tsang F, Dilova I, Wang C, Lu SP, et al. (2007) The dihydrolipoamide acetyltransferase is a novel metabolic longevity factor and is required for calorie restriction-mediated life span extension. J Biol Chem 282: 6161–6171.
- 54. Tsang CK, Li H, Zheng XS (2007) Nutrient starvation promotes condensin loading to maintain rDNA stability. EMBO J 26: 448–458.
- 55. Morrow G, Battistini S, Zhang P, Tanguay RM (2004) Decreased lifespan in the absence of expression of the mitochondrial small heat shock protein Hsp22 in Drosophila. J Biol Chem 279: 43382–43385.
- 56. Selsby JT, Judge AR, Yimlamai T, Leeuwenburgh C, Dodd SL (2005) Life long calorie restriction increases heat shock proteins and proteasome activity in soleus muscles of Fisher 344 rats. Exp Gerontol 40: 37–42.
- 57. Longo VD (2003) The Ras and Sch9 pathways regulate stress resistance and longevity. Exp Gerontol 38: 807–811.
- 58. Fabrizio P, Pletcher SD, Minois N, Vaupel JW, Longo VD (2004) Chronological aging-independent replicative life span regulation by Msn2/Msn4 and Sod2 in Saccharomyces cerevisiae. FEBS Lett 557: 136–142.
- 59. Fabrizio P, Gattazzo C, Battistella L, Wei M, Cheng C, et al. (2005) Sir2 blocks extreme life-span extension. Cell 123: 655–667.
- 60. Masoro EJ (2000) Hormesis is the beneficial action resulting from the response of an organism to a low-intensity stressor. Hum Exp Toxicol 19: 340–341.
- 61. Sinclair DA (2005) Toward a unified theory of caloric restriction and longevity regulation. Mech Ageing Dev 126: 987–1002.
- 62. Bitterman KJ, Medvedik O, Sinclair DA (2003) Longevity regulation in Saccharomyces cerevisiae: Linking metabolism, genome stability, and heterochromatin. Microbiol Mol Biol Rev 67: 376–399.
- 63. Mostoslavsky R, Chua KF, Lombard DB, Pang WW, Fischer MR, et al. (2006) Genomic instability and aging-like phenotype in the absence of mammalian SIRT6. Cell 124: 315–329.
- 64. Lamming DW, Latorre-Esteves M, Medvedik O, Wong SN, Tsang FA, et al. (2006) Response to comment on “HST2 mediates SIR2-independent life-span extension by calorie restriction.” Science 312: 1312.
- 65. Longtine MS, McKenzie A 3rd, Demarini DJ, Shah NG, Wach A, et al. (1998) Additional modules for versatile and economical PCR-based gene deletion and modification in Saccharomyces cerevisiae. Yeast 14: 953–961.
- 66. Goldstein AL, McCusker JH (1999) Three new dominant drug resistance cassettes for gene disruption in Saccharomyces cerevisiae. Yeast 15: 1541–1553.
- 67. Heitman J, Movva NR, Hall MN (1991) Targets for cell cycle arrest by the immunosuppressant rapamycin in yeast. Science 253: 905–909.
- 68. Strahl-Bolsinger S, Hecht A, Luo K, Grunstein M (1997) SIR2 and SIR4 interactions differ in core and extended telomeric heterochromatin in yeast. Genes Dev 11: 83–93.
- 69. Huang J, Moazed D (2003) Association of the RENT complex with nontranscribed and coding regions of rDNA and a regional requirement for the replication fork block protein Fob1 in rDNA silencing. Genes Dev 17: 2162–2176.