Metallochaperone UreG serves as a new target for design of urease inhibitor: A novel strategy for development of antimicrobials
Fig 4
Binding of cmpd4/cmpd8 to UreG by fluorescence measurement and docking models.
(A) Fluorescence spectra of UreG upon addition of cmpd4. UreG displays fluorescence at 310 nm and 335 nm in the absence of cmpd4; binding of cmpd4 to UreG induced the fluorescence quenching of UreG. (B) Fluorescence spectra of UreG upon addition of cmpd8. (C)(D) The representative binding curves of cmpd4 and cmpd8 to UreG. (E)(F) cmpd4 and cmpd8 bind to the G1(P-loop) motif (magentas) and G4 (NKXD) motif in guanine nucleotide binding pocket of UreG. The underlying data for Fig 4C and 4D can be found in S1 Data.