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Lef1-dependent hypothalamic neurogenesis inhibits anxiety

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Hypothalamic Lef1 regulates growth and anxiety in mice.

(A) Body weight of male Lef1CKO (CKO-M, n = 27) and female Lef1CKO (CKO-F, n = 26) compared to controls (CON-M, n = 27; CON-F, n = 26). (B) Elevated plus maze (EPM). (C) Open field test (OFT). In (B) and (C), n = 12, 9 for male CON, CKO. In (B), n = 11, 11 for female CON, CKO in estrus; n = 12, 11 for female CON, CKO in diestrus. In (C), n = 12, 6 for female CON, CKO in estrus; n = 11, 16 for female CON, CKO in diestrus. Data are mean ± 95% CI (A) or SEM (B and C). ***P < 0.001, **P < 0.01, *P < 0.05, ns. P > 0.05 by 2-way ANOVA with repeated measures (A, F(1, 26) = 22.2 for male and F(1, 25) = 8.842 for female) and unpaired Student t tests (B and C). Outliers depicted in black (C) were excluded using the Grubbs’ test (P < 0.05). Raw data can be found in S1 Data.

Fig 4

doi: https://doi.org/10.1371/journal.pbio.2002257.g004